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  • 1
    ISSN: 1432-0983
    Keywords: Key words Ascochyta ; Pea ; Isozymes ; rDNA spacers
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract  Isolates of three closely related pea pathogens, Ascochyta pisi, Ascochyta pinodes (teleomorph Mycosphaerella pinodes) and Phoma medicaginis var. pinodella, were compared by means of isozyme analysis and restriction fragment length polymorphisms (RFLP) of amplified rDNA spacers. Three enzyme systems differentiated A. pisi from M. pinodes and P. m. pinodella. The internal transcribed spacers (ITSs) of the three fungi showed no intraspecific and very little interspecific variation after digestion with 12 endonucleases. Digestion of the intergenic spacer (IGS) with Hinf I, and Sau3A revealed uniformity in A. pisi patterns which consistently differed from those of M. pinodes and P. m. pinodella. No clear distinction could be made between the latter two fungi which both showed intraspecific variability. Both biochemical and molecular markers thus discriminated between two Ascochyta species. The results also indicated a closer relationship between two organisms belonging to different genera (Ascochyta and Phoma) than between two species of the same genus (Ascochyta).
    Type of Medium: Electronic Resource
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  • 2
    ISSN: 1432-0983
    Keywords: Ascochyta ; Pea ; Isozymes ; rDNA spacers
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Isolates of three closely related pea pathogens,Ascochyta pisi, Ascochyta pinodes (teleomorphMycosphaerella pinodes) andPhoma medicaginis var. pinodella, were compared by means of isozyme analysis and restriction fragment length polymorphisms (RFLP) of amplified rDNA spacers. Three enzyme systems differentiatedA. pisi fromM. pinodes andP. m. pinodella. The internal transcribed spacers (ITSs) of the three fungi showed no intraspecific and very little interspecific variation after digestion with 12 endonucleases. Digestion of the intergenic spacer (IGS) withHinfI, andSau3A revealed uniformity inA. pisi patterns which consistently differed from those ofM. pinodes andP. m. pinodella. No clear distinction could be made between the latter two fungi which both showed intraspecific variability. Both biochemical and molecular markers thus discriminated between twoAscochyta species. The results also indicated a closer relationship between two organisms belonging to different genera (Ascochyta andPhoma) than between two species of the same genus (Ascochyta).
    Type of Medium: Electronic Resource
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  • 3
    Electronic Resource
    Electronic Resource
    Amsterdam : Elsevier
    Phytochemistry 36 (1994), S. 691-693 
    ISSN: 0031-9422
    Keywords: Marine microalgae ; cosmetics. ; polyunsaturated fatty acids
    Source: Elsevier Journal Backfiles on ScienceDirect 1907 - 2002
    Topics: Biology , Chemistry and Pharmacology
    Type of Medium: Electronic Resource
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  • 4
    Electronic Resource
    Electronic Resource
    Springer
    Entomologia experimentalis et applicata 80 (1996), S. 197-201 
    ISSN: 1570-7458
    Keywords: primary metabolites ; sugars ; amino acids ; leaf cuticle ; wax
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Type of Medium: Electronic Resource
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  • 5
    ISSN: 1573-8248
    Keywords: biology ; longevity ; ovolarviposition intensity ; ovolarviposition period ; ovolarviposition rhythm ; larval parasitoid
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Description / Table of Contents: Résumé Le tachinaire ovolarviparePseudoperichaeta nigrolineata Walker est un parasitoïde larvaire de la pyrale du maïs. Une étude des comportements des femelles gravides et des kairomones qui influent sur ces comportements nous a conduit à quantifier plusieurs caractéristiques biologiques de ces femelles. Les femelles sont mises en expérience à partir du moment où elles sont gravides, en présence du complexe maïs-chenille changé trois fois par 24 H. Elles vivent en moyenne 8,5 jours. A partir de l'émergence, la longévité moyenne est de 24 jours et la longévité maximale, de 39 jours. La durée globale de la période de ponte est de 12 jours. Par contre, le nombre réel de jours de ponte par femelle est en moyenne de 6,9. Le nombre de jours séparant la fin de la ponte du jour de la mort est variable, mais 69% pondent jusqu' à leur mort. Toutes les femelles ont pondu, certaines régulièrement (59%), d'autres avec des interruptions (41%). La ponte totale moyenne par femelle est de 83 larves, avec des extrêmes de 15 et 231 larves. Trente pour cent des femelles pondent entre 30 et 60 planidia. La variabilité est forte quel que soit le caractère étudié. Ces résultats sont discutés par rapport à d'autres tachinaires et aux conditions expérimentales.
    Notes: Abstract The ovolarviparous tachinid flyPseudoperichaeta nigrolineata Walker is a larval parasitoid of the European corn borer,Ostrinia nubilalis Hübner. A study of the behaviours of gravid females and the kairomones that influence these behaviours led us to quantify several biological characteristics of these females. The females were observed from the moment they became gravid. They were placed in the presence of a corn-caterpillar complex that was changed three times per 24-hour period. Parasitoids lived an average of 8.5 more days. From emergence, the mean longevity was 24 days and the maximum, 39 days. The ovolarviposition period of the sample lasted a total of 12 days. However, the actual number of days of ovolarviposition per female averaged 6.9. The number of days between the end of ovolarviposition and death varies, but 69% of females laid eggs until their death. All the females ovolarviposited; some laid eggs regularly (59%) and others with interruptions (41%). Total mean ovolarviposition per female was 83 maggots, with extremes of 15 and 231 maggots. Thirty percent of the females laid eggs between 30 and 60 planidia. Variability was high for all characteristics studied. Results obtained are discussed with reference to other tachinids and the experimental conditions used.
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  • 6
    Publication Date: 2016-12-16
    Keywords: Immunobiology, Lymphoid Neoplasia, Gene Therapy
    Print ISSN: 0006-4971
    Electronic ISSN: 1528-0020
    Topics: Biology , Medicine
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  • 7
    Publication Date: 2020-09-30
    Description: The cosmopolitan, potentially toxic dinoflagellate Protoceratium reticulatum possesses a fossilizable cyst stage which is an important paleoenvironmental indicator. Slight differences in the internal transcribed spacer ribosomal DNA (ITS rDNA) sequences of P. reticulatum have been reported, and both the motile stage and cyst morphology of P. reticulatum display phenotypic plasticity, but how these morpho-molecular variations are related with ecophysiological preferences is unknown. Here, 55 single cysts or cells were isolated from localities in the Northern (Arctic to subtropics) and Southern Hemispheres (Chile and New Zealand), and in total 34 strains were established. Cysts and/or cells were examined with light microscopy and/or scanning electron microscopy. Large subunit ribosomal DNA (LSU rDNA) and/or ITS rDNA sequences were obtained for all strains/isolates. All strains/isolates of P. reticulatum shared identical LSU sequences except for one strain from the Mediterranean Sea that differs in one position, however ITS rDNA sequences displayed differences at eight positions. Molecular phylogeny was inferred using maximum likelihood and Bayesian inference based on ITS rDNA sequences. The results showed that P. reticulatum comprises at least three ribotypes (designated as A, B, and C). Ribotype A included strains from the Arctic and temperate areas, ribotype B included strains from temperate regions only, and ribotype C included strains from the subtropical and temperate areas. The average ratios of process length to cyst diameter of P. reticulatum ranged from 15% in ribotype A, 22% in ribotype B and 17% in ribotype C but cyst size could overlap. Theca morphology was indistinguishable among ribotypes. The ITS-2 secondary structures of ribotype A displayed one CBC (compensatory change on two sides of a helix pairing) compared to ribotypes B and C. Growth response of one strain from each ribotype to various temperatures was examined. The strains of ribotypes A, B and C exhibited optimum growth at 15 °C, 20 °C and 20–25 °C, respectively, thus corresponding to cold, moderate and warm ecotypes. The profiles of yessotoxins (YTXs) were examined for 25 strains using liquid chromatography coupled with tandem mass spectrometry (LC–MS/MS). The parent compound yessotoxin (YTX) was produced by strains of ribotypes A and B, but not by ribotype C strains, which only produced the structural variant homoyessotoxin (homoYTX). Our results support the notion that there is significant intra-specific variability in Protoceratium reticulatum and the biogeography of the different ribotypes is consistent with specific ecological preferences.
    Repository Name: EPIC Alfred Wegener Institut
    Type: Article , isiRev
    Format: application/pdf
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  • 8
    Publication Date: 2020-06-17
    Description: Peripheral diffuse degassing of CO2 from the soil occurs across the western flank of Piton de la Fournaise volcano (La Réunion Island, Indian Ocean) along a narrow zone. In this area, carbon isotopic analysis on soil gas samples highlights significant mixing between magmatic and organic end-members. The zones with the strongest magmatic signature (highest δ13C) overlap spatial distribution of hypocenters recorded shortly before and during volcano reactivation and allow discriminating a N135° degassing lineament, with a minimum length of 11 km and 140 ± 20 m-width. Such orientation is in accordance with that of an old dyke network along the rift zone and with N120–130° and N140–155° lineaments related to the inheritance of oceanic lithosphere structures. Our findings show that this N135° lineament represents a preferential magmatic pathway for deep magma transfer below the volcano flank. Moreover, spatial distributions of recent eccentric cones indicate a well-founded possibility that future eruptions may by-pass the shallow plumbing system of the central area of the volcano, taking a lateral pathway along this structure. Our results also confirm that Piton de la Fournaise activity is linked to a laterally shifted plumbing system and represent a major improvement in identifying the main high-risk area on the densely populated western flank of the volcano
    Description: Published
    Description: 193-207
    Description: 2V. Struttura e sistema di alimentazione dei vulcani
    Description: 4V. Dinamica dei processi pre-eruttivi
    Description: 5V. Dinamica dei processi eruttivi e post-eruttivi
    Description: 6V. Pericolosità vulcanica e contributi alla stima del rischio
    Description: JCR Journal
    Repository Name: Istituto Nazionale di Geofisica e Vulcanologia (INGV)
    Type: article
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  • 9
    Publication Date: 2023-10-26
    Description: Preliminary interpretation of geological processes during field measurement campaigns require fast data analysis to adapt ongoing target strategies. It is the case of soil investigations where coupling geochemical and geophysical records favors a better understanding of subsurface processes. This task requires (i) statistical analysis to identify areas of interest during spatial surveys and (ii) signal processing to analyze temporal series. Here we present SoilExp, an open-source Python-based Graphical User Interface (GUI) that permits to process spatial and temporal surveys of soil gases (e.g. soil CO2 flux) combined with common physical parameters (e.g. self-potential, temperature) that are synchronously recorded on the field. SoilExp mixes innovative algorithms with the more common tools used for the analysis of both spatial surveys or temporal series. It offers the possibility to display distribution plots, maps, comparative plots, spectra and spectrograms, as well as data statistical analysis, in order to deal efficiently with datasets acquired on the field. Field measurements performed at Stromboli (Italy) supports that such software solution facilitates a quick visualization of the data output and is a powerful tool on the geochemical and geophysical analysis.
    Description: Published
    Description: 104553
    Description: 4V. Processi pre-eruttivi
    Description: 5V. Processi eruttivi e post-eruttivi
    Description: 6V. Pericolosità vulcanica e contributi alla stima del rischio
    Description: JCR Journal
    Repository Name: Istituto Nazionale di Geofisica e Vulcanologia (INGV)
    Type: article
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  • 10
    Publication Date: 2020-02-12
    Language: English
    Type: info:eu-repo/semantics/workingPaper
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