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  • 1
    Electronic Resource
    Electronic Resource
    Differentiation of the “Ascochyta complex” fungi of pea by biochemical and molecular markers (1996)
    Springer
    Current genetics 29 (1996), S. 182-190 
    Details
    ISSN: 1432-0983
    Keywords: Key words Ascochyta ; Pea ; Isozymes ; rDNA spacers
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract  Isolates of three closely related pea pathogens, Ascochyta pisi, Ascochyta pinodes (teleomorph Mycosphaerella pinodes) and Phoma medicaginis var. pinodella, were compared by means of isozyme analysis and restriction fragment length polymorphisms (RFLP) of amplified rDNA spacers. Three enzyme systems differentiated A. pisi from M. pinodes and P. m. pinodella. The internal transcribed spacers (ITSs) of the three fungi showed no intraspecific and very little interspecific variation after digestion with 12 endonucleases. Digestion of the intergenic spacer (IGS) with Hinf I, and Sau3A revealed uniformity in A. pisi patterns which consistently differed from those of M. pinodes and P. m. pinodella. No clear distinction could be made between the latter two fungi which both showed intraspecific variability. Both biochemical and molecular markers thus discriminated between two Ascochyta species. The results also indicated a closer relationship between two organisms belonging to different genera (Ascochyta and Phoma) than between two species of the same genus (Ascochyta).
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s002940050034
    Location Call Number Limitation Availability
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    Paper (German National Licenses)
    Fulltext
  • 2
    Electronic Resource
    Electronic Resource
    Differentiation of the “Ascochyta complex” fungi of pea by biochemical and molecular markers (1996)
    Springer
    Current genetics 29 (1996), S. 182-190 
    Details
    ISSN: 1432-0983
    Keywords: Ascochyta ; Pea ; Isozymes ; rDNA spacers
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Isolates of three closely related pea pathogens,Ascochyta pisi, Ascochyta pinodes (teleomorphMycosphaerella pinodes) andPhoma medicaginis var. pinodella, were compared by means of isozyme analysis and restriction fragment length polymorphisms (RFLP) of amplified rDNA spacers. Three enzyme systems differentiatedA. pisi fromM. pinodes andP. m. pinodella. The internal transcribed spacers (ITSs) of the three fungi showed no intraspecific and very little interspecific variation after digestion with 12 endonucleases. Digestion of the intergenic spacer (IGS) withHinfI, andSau3A revealed uniformity inA. pisi patterns which consistently differed from those ofM. pinodes andP. m. pinodella. No clear distinction could be made between the latter two fungi which both showed intraspecific variability. Both biochemical and molecular markers thus discriminated between twoAscochyta species. The results also indicated a closer relationship between two organisms belonging to different genera (Ascochyta andPhoma) than between two species of the same genus (Ascochyta).
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF02221583
    Location Call Number Limitation Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
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