In:
PLOS ONE, Public Library of Science (PLoS), Vol. 17, No. 11 ( 2022-11-8), p. e0276904-
Abstract:
The early diagnosis of hepatocellular carcinoma (HCC) has not been clinically elucidated, leading to an increased mortality rate in patients with HCC. HCC is a systemic disease related to disorders of blood homeostasis, and the association between red blood cells (RBCs) and HCC tumorigenesis remains elusive. We performed data-independent acquisition proteomic analyses of 72 clinical RBC samples, including HCC (n = 30), liver cirrhosis (LC, n = 17), and healthy controls (n = 25), and characterized the clinical relevance of RBCs and tumorigenesis in HCC. We observed dynamic changes in RBCs during HCC tumorigenesis, and our findings indicate that, based on the protein expression profiles of RBCs, LC is a developmental stage closely approaching HCC. The expression of hemoglobin (HbA and HbF) in peripheral blood dynamically changed during HCC tumorigenesis, suggesting that immature erythroid cells exist in peripheral blood of HCC patients and that erythropoiesis is influenced by the onset of LC. We also identified the disrupted autophagy pathway in RBCs at the onset of LC, which persisted during HCC tumorigenesis. The oxytocin and GnRH pathways were disrupted and first identified during the development of LC into HCC. Significantly differentially expressed SMIM1, ANXA7, HBA1, and HBE1 during tumorigenesis were verified as promising biomarkers for the early diagnosis of HCC using parallel reaction monitoring technology. This study may enhance the understanding of HCC tumorigenesis from a different point of view and aid the early diagnosis of HCC.
Type of Medium:
Online Resource
ISSN:
1932-6203
DOI:
10.1371/journal.pone.0276904
DOI:
10.1371/journal.pone.0276904.g001
DOI:
10.1371/journal.pone.0276904.g002
DOI:
10.1371/journal.pone.0276904.g003
DOI:
10.1371/journal.pone.0276904.g004
DOI:
10.1371/journal.pone.0276904.g005
DOI:
10.1371/journal.pone.0276904.g006
DOI:
10.1371/journal.pone.0276904.s001
DOI:
10.1371/journal.pone.0276904.s002
DOI:
10.1371/journal.pone.0276904.s003
DOI:
10.1371/journal.pone.0276904.s004
DOI:
10.1371/journal.pone.0276904.s005
DOI:
10.1371/journal.pone.0276904.s006
DOI:
10.1371/journal.pone.0276904.s007
DOI:
10.1371/journal.pone.0276904.s008
Language:
English
Publisher:
Public Library of Science (PLoS)
Publication Date:
2022
detail.hit.zdb_id:
2267670-3
Permalink