A Multiplex RT-PCR Assay for Detection and Differentiation of Avian-Origin Canine H3N2, Equine-Origin H3N8, Human-Origin H3N2, and H1N1/2009 Canine Influenza Viruses
Fig 3
Sensitivity of the mRT-PCR assay.
(A) Lanes 1–7: Individual equine-origin H3N8 (A/canine/Colorado/6723-8/2008), hH3N2 (A/Jiangxi/262/2005), H1N1/2009 (A/canine/Beijing/cau2/2009), and cH3N2 (A/canine/Beijing/364/2009) viruses were tested at 10-fold dilutions ranging from 1 × 105 to 1× 10−1 TCID50/100 μl. Lane 8: negative control allantoic fluid. (B) Lanes 1–6: A mix of the same four viruses were tested using serial 10-fold dilutions from 1 × 105 to 1 × 100 TCID50/100 μl. Lane M: molecular marker; Lane 7: negative control allantoic fluid.