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  • 1
    Electronic Resource
    Electronic Resource
    Springer
    Transgenic research 7 (1998), S. 403-411 
    ISSN: 1573-9368
    Keywords: transgenic organism ; gene expression ; promoter ; plant ; gene regulation ; heterologous system
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The regulation of gene expression represents a specific process which has different structural and functional requirements in different groups of organisms. It is thus assumed that regulatory sequences of eucaryotes cannot be recognized in procaryotes. This assumption is of interest for risk assessments of the environmental impact of deliberate release experiments with genetically modified organisms. In order to analyse the extent of heterologous gene expression caused by the transfer of plant-specific regulatory sequences into bacteria, we constructed fusions between plant-specific regulatory sequences and the coding regions of the luxAB genes for the luciferase of the bioluminescent bacterium Vibrio harveyi, transferred the fusions into different bacterial species and measured the luminescence to quantify the expression of the luciferase genes. The regulatory sequences investigated included (a) the 35S promoter of the Cauliflower mosaic virus, (b) the B33 promoter of a class I patatin gene of potatoes, (c) the promoter of the ST-LS1 gene of potatoes and (d) the promoter of the rolC gene of Agrobacterium rhizogenes. We could show that in addition to the 35S promoter, which has already been described as being recognized in Escherichia coli, the sequences containing the B33 and the ST-LS1 promoters are recognized in bacteria. Luciferase gene expression promoted by the sequence with the ST-LS1 promoter could be observed in E. coli, Yersinia enterocolitica and Agrobacterium tumefaciens. Comparison of the luminescence caused by fusions between luxAB and different promoters on the chromosome and on an endogenous plasmid of Y. enterocolitica demonstrated that the level of the heterologous gene expression caused by the fragment with the ST-LS1 promoter was within the range of gene expression levels caused by endogenous promoters of Y. enterocolitica.
    Type of Medium: Electronic Resource
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  • 2
    Publication Date: 2011-12-21
    Print ISSN: 0941-2948
    Electronic ISSN: 1610-1227
    Topics: Geography , Physics
    Published by Schweizerbart
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  • 3
    Publication Date: 2017-03-20
    Description: West African summer monsoon precipitation is characterized by distinct decadal variability. Due to its well-documented link to oceanic boundary conditions in various ocean basins it represents a paradigm for decadal predictability. In this study, we reappraise this hypothesis for several sub-regions of sub-Saharan West Africa using the new German contribution to the coupled model intercomparison project phase 5 (CMIP5) near-term prediction system. In addition, we assume that dynamical downscaling of the global decadal predictions leads to an enhanced predictive skill because enhanced resolution improves the atmospheric response to oceanic forcing and land-surface feedbacks. Based on three regional climate models, a heterogeneous picture is drawn: none of the regional climate models outperforms the global decadal predictions or all other regional climate models in every region nor decade. However, for every test case at least one regional climate model was identified which outperforms the global predictions. The highest predictive skill is found in the western and central Sahel Zone with correlation coefficients and mean-square skill scores exceeding 0.9 and 0.8, respectively.
    Repository Name: EPIC Alfred Wegener Institut
    Type: Article , isiRev
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  • 4
    Publication Date: 2021-05-25
    Description: For the first time for CORDEX-South Asia, a high-resolution regional earth system model (ROM) is adopted to assess the impact of horizontal resolution (0.22◦ and 0.11◦) in simulating the Indian summer monsoon rainfall (ISMR) and the underlying spatiotemporal variability. ROM at both resolutions bears a close resemblance to observations in simulating the mean precipitation climatology compared to other regional climate models (RCMs) participated in CORDEX- South Asia. ROM shows substantial improvement relative to the ensemble mean of the RCMs included in CORDEX-South Asia. While comparing both simulations with observations, some sys-tematic wet and dry bias over Central India (CI) and Northern Western Ghats is noticed. In general, the wet/dry bias over India is mainly associated with the overestimation/underestimation of the large-scale/convective component. Increasing horizontal resolution from 0.22◦ to 0.11◦ significantly adds value in simulating the JJAS mean precipitation by reducing the wet bias over western central India (WCI) and southern peninsular India and dry bias over eastern CI. The reduction in wet/dry bias is mainly associated with suppression/enhancement of the large scale/convective precipitation. This improvement in mean precipitation is partially due to the improved representation of the propagation of mesoscale systems such as boreal summer intraseasonal oscilla-tion (eastward and northward). Despite the above improvements, the wet precipitation bias, particularly over WCI, persists. The weaker Findlater Jet associated with weaker land-ocean thermal contrast caused by the warm sea surface temperature (SST) bias over the western Arabian Sea (AS) suggests that AS moisture transport does not contribute to the wet bias over India. The wet bias is possibly associated with favourable atmospheric conditions (atmospheric instability).
    Repository Name: EPIC Alfred Wegener Institut
    Type: Article , isiRev
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  • 5
    Publication Date: 2021-05-25
    Description: In this work we use a regional atmosphere–ocean coupled model (RAOCM) and its stand-alone atmospheric component to gain insight into the impact of atmosphere–ocean coupling on the climate change signal over the Iberian Peninsula (IP). The IP climate is influenced by both the Atlantic Ocean and the Mediterranean sea. Complex interactions with the orography take place there and high-resolution models are required to realistically reproduce its current and future climate. We find that under the RCP8.5 scenario, the generalized 2-m air temperature (T2M) increase by the end of the twenty-first century (2070–2099) in the atmospheric-only simulation is tempered by the coupling. The impact of coupling is specially seen in summer, when the warming is stronger. Precipitation shows regionally-dependent changes in winter, whilst a drier climate is found in summer. The coupling generally reduces the magnitude of the changes. Differences in T2M and precipitation between the coupled and uncoupled simulations are caused by changes in the Atlantic large-scale circulation and in the Mediterranean Sea. Additionally, the differences in projected changes of T2M and precipitation with the RAOCM under the RCP8.5 and RCP4.5 scenarios are tackled. Results show that in winter and summer T2M increases less and precipitation changes are of a smaller magnitude with the RCP4.5. Whilst in summer changes present a similar regional distribution in both runs, in winter there are some differences in the NW of the IP due to differences in the North Atlantic circulation. The differences in the climate change signal from the RAOCM and the driving Global Coupled Model show that regionalization has an effect in terms of higher resolution over the land and ocean.
    Repository Name: EPIC Alfred Wegener Institut
    Type: Article , isiRev
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  • 6
    Publication Date: 2020-07-06
    Description: Climate simulations for the North Atlantic and Europe for recent and future conditions simulated with the regionally coupled ROM model are analyzed and compared to the results from the MPI‐ESM. The ROM simulations also include a biogeochemistry and ocean tides. For recent climate conditions, ROM generally improves the simulations compared to the driving model MPI‐ESM. Reduced oceanic biases in the Northern Atlantic are found, as well as a better simulation of the atmospheric circulation, notably storm tracks and blocking. Regarding future climate projections for the 21st century following the RCP 4.5 and 8.5 scenarios, MPI‐ESM and ROM largely agree qualitatively on the climate change signal over Europe. However, many important differences are identified. For example, ROM shows an SST cooling in the Subpolar Gyre which is not present in MPI‐ESM. Under the RCP8.5 scenario, ROM Arctic sea ice cover is thinner and reaches the seasonally ice‐free state by 2055, well before MPI‐ESM. This shows the decisive importance of higher ocean resolution and regional coupling for determining the regional responses to global warming trends. Regarding biogeochemistry, both ROM and MPI‐ESM simulate a widespread decline in winter nutrient concentration in the North Atlantic of up to ~35%. On the other hand, the phytoplankton spring bloom in the Arctic and in the North‐Western Atlantic starts earlier and the yearly primary production is enhanced in the Arctic in the late 21st century. These results clearly demonstrate the added value of ROM to determine more detailed and more reliable climate projections at the regional scale.
    Repository Name: EPIC Alfred Wegener Institut
    Type: Article , isiRev
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  • 7
    Publication Date: 2020-08-25
    Description: Changes of surface air temperature (SAT) over the Indochina Peninsula (ICP) under the Representative Concentration Pathway (RCP) 8.5 scenario are projected for wet and dry seasons in the short-term (2020–2049) and long-term (2070–2099) future of the twenty-first century. A first analysis on projections of the SAT by the state-of-the-art regionally coupled atmosphere-ocean model ROM, including exchanges of momentum, heat, and water fluxes between the atmosphere (Regional Model) and ocean (Max Planck Institute Ocean Model) models, shows the following results: (i) In both seasons, the highest SAT occurs over the southern coastal area while the lowest over the northern mountains. The highest warming magnitudes are located in the northwestern part of the ICP. The regionally averaged SAT over the ICP increases by 2.61 °C in the wet season from short- to long-term future, which is slightly faster than that of 2.50 °C in the dry season. (ii) During the short-term future, largest SAT trends occur over the southeast and northwest ICP in wet and dry seasons, respectively. On regional average, the wet season is characterized by a significant warming rate of 0.22 °C decade−1, while it is non-significant with 0.11 °C decade−1for the dry season. For the long-term future, the rapid warming is strengthened significantly over whole ICP, with trends of 0.51 °C decade−1and 0.42 °C decade−1in wet and dry seasons,respectively. (iii) In the long-term future, more conspicuous warming is noted, especially in the wet season, due to the increased downward longwave radiation. Higher CO2concentrations enhancing the greenhouse effect can be attributed to the water vapor–greenhouse feedback, which, affecting atmospheric humidity and counter radiation, leads to the rising SAT.
    Repository Name: EPIC Alfred Wegener Institut
    Type: Article , isiRev
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  • 8
    Publication Date: 2018-12-10
    Description: The ability of German regional climate Model (REMO) to simulate the near surface air temperature and total precipitation over China in 1989-2008 were assessed with the use of Taylor diagrams and bias analysis. Comparing the simulated near surface air temperature with a 20-year observational dataset from China, the spatial correlation coefficient was relatively high (0.94). However, the spatial correlation coefficient for total precipitation was relatively low (about 0.42). The near surface air temperature simulated by REMO was higher than the observed values in most part of China, showing a bias range within 4 C. Significant cold bias of about -4 to -2 C occurred over most of the Qinghai-Tibetan Plateau. In terms of total precipitation, the simulated values were higher than the observed ones, with biases evenly distributed. The annual mean bias in most part of China was within 300 mm. Except for the Qinghai-Tibetan Plateau, South China and Southwest China, REMO accurately reflected the distribution of near surface air temperature and total precipitation. REMO represented the temperature and total precipitation well in North China and Northeast China. REMO simulations were quite close to observations for near surface air temperature in summer and total precipitation in winter. REMO still needs to be improved in complex terrain areas.
    Repository Name: EPIC Alfred Wegener Institut
    Type: Article , peerRev
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  • 9
    Publication Date: 2016-11-18
    Repository Name: EPIC Alfred Wegener Institut
    Type: Article , isiRev
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  • 10
    Publication Date: 2018-03-26
    Repository Name: EPIC Alfred Wegener Institut
    Type: Article , isiRev
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