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  • 1990-1994  (2)
  • 1
    Electronic Resource
    Electronic Resource
    Synthesis in vitro of very long chain fatty acids in Vibrio sp. strain ABE-1 (1992)
    Springer
    Archives of microbiology 157 (1992), S. 223-228 
    Details
    ISSN: 1432-072X
    Keywords: Fatty acids synthetase ; Very long chain fatty acids ; Psychrophilic bacteria ; Vibrio sp
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The activity of fatty acid synthetase (FAS) from Vibrio sp. strain ABE-1 required the presence of acyl carrier protein and was completely inhibited by thiolactomycin, an inhibitor specific for a type II FAS. These observations indicate that this enzyme is a type II FAS. Analysis by gas-liquid chromotography of the reaction products synthesized in vitro from [2-14C]malonyl-CoA by the partially purified FAS revealed, in addition to 16-and 18-carbon fatty acids which are normal constituents of this bacterium, the presence of fatty acids with very long chains. These fatty acids were identified as saturated and mono-unsaturated fatty acids with 20 up to as many as 30 carbon atoms. The longest fatty acids normally found in this bacterium contain 18-carbon atoms. These results suggest that the FAS from Vibrio sp. strain ABE-1 has potentially the ability to synthesize fatty acids with very long chains.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00245154
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    Paper (German National Licenses)
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  • 2
    Electronic Resource
    Electronic Resource
    Lipoamino acids and sulfonoplipids in Cytophaga johnsonae Stanier strain C21 and six related species of Cytophaga (1992)
    Springer
    Archives of microbiology 158 (1992), S. 171-175 
    Details
    ISSN: 1432-072X
    Keywords: Lipoamino acid ; Glycine lipid ; Ornithine lipid ; Sulfonolipid ; Chemosystematic marker ; Cytophaga spp
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Cytophaga johnsonae Stanier strain C21 (C. johnsonae C21) contains phosphatidylethanolamine (PE), an unusual glycine-containing lipid (glycine lipid), and two kinds of unidentified lipid as major lipid components. One of the latter lipids was identified by chemical and physicochemical methods as iso-3-hydroxy fatty acid, α-amide linked to ornithine and esterified to iso-nonhydroxy fatty acid (ornithine lipid). The other lipid was identified as a sulfonolipid by a tracer experiment using 35S. PE, glycine lipid and sulfonolipid were found in all seven species of Cytophage examined, namely, C. huchinsonii, C. heparina, C. johnsonae C21, C. aquatilis, and three unidentified species of Cytophaga. However, ornithine lipid was found only in the latter five species. By contrast, a serine-containing lipid, which is a specific lipid component of Flavobacterium species, was not found in any species of Cytophaga examined. The possible use and significance of amino acid-containing lipids and sulfonolipids as chemosystematic markers of the Cytophaga species are discussed.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00290812
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    Paper (German National Licenses)
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