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  • 1
    Electronic Resource
    Electronic Resource
    Na+-driven ATP synthesis of a psychrophilic marine bacterium, Vibrio sp. strain ABE-1 (1991)
    Oxford, UK : Blackwell Publishing Ltd
    FEMS microbiology letters 82 (1991), S. 0 
    Details
    ISSN: 1574-6968
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Notes: Abstract: In vivo ATP synthesis of a psychrophilic marine bacterium, Vibrio sp. strain ABE-1, derived from endogenous respiration, was examined. ATP was synthesized at both pH 6.5 and 8.5 after the start of the endogenous respiration by supplying O2 to the anaerobic cell suspension. The ATP synthesis at pH 6.5, but not at pH 8.5, was completely inhibited by a H+ conductor, carbonylcyanide m-chlorophenylhydrazone (CCCP). The CCCP-resistant ATP synthesis at pH 8.5 was strongly inhibited by an inhibitor of the respiration-dependent primary Na+ pump, 2-n-heptyl-4-hydroxyquinolineN-oxide, and essentially required Na+. These results show that this bacterium synthesizes ATP at pH 6.5 by electrochemical potentials across the membrane Δ∼μH+, whereas at pH 8.5 by Δ∼μNa+ but not Δ∼μH+.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1574-6968.1991.tb04869.x
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  • 2
    Electronic Resource
    Electronic Resource
    Degradative Pathway of L -Leucine in Proteus vulgaris (1961)
    [s.l.] : Nature Publishing Group
    Nature 189 (1961), S. 400-400 
    Details
    ISSN: 1476-4687
    Source: Nature Archives 1869 - 2009
    Topics: Biology , Chemistry and Pharmacology , Medicine , Natural Sciences in General , Physics
    Notes: [Auszug] Proteus vulgaris was cultivated at 37 C. for 18 hr. on peptone-broth agar slant. The cells were harvested, washed three times with de-ionized water, and suspended in 2 X 10~2 M phosphate buffer, pH. 7 -2. The washed cell suspension was disrupted by sonic oscillation in a Kubota vibrator, 10 ...
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1038/189400a0
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  • 3
    Electronic Resource
    Electronic Resource
    Synthesis in vitro of very long chain fatty acids in Vibrio sp. strain ABE-1 (1992)
    Springer
    Archives of microbiology 157 (1992), S. 223-228 
    Details
    ISSN: 1432-072X
    Keywords: Fatty acids synthetase ; Very long chain fatty acids ; Psychrophilic bacteria ; Vibrio sp
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The activity of fatty acid synthetase (FAS) from Vibrio sp. strain ABE-1 required the presence of acyl carrier protein and was completely inhibited by thiolactomycin, an inhibitor specific for a type II FAS. These observations indicate that this enzyme is a type II FAS. Analysis by gas-liquid chromotography of the reaction products synthesized in vitro from [2-14C]malonyl-CoA by the partially purified FAS revealed, in addition to 16-and 18-carbon fatty acids which are normal constituents of this bacterium, the presence of fatty acids with very long chains. These fatty acids were identified as saturated and mono-unsaturated fatty acids with 20 up to as many as 30 carbon atoms. The longest fatty acids normally found in this bacterium contain 18-carbon atoms. These results suggest that the FAS from Vibrio sp. strain ABE-1 has potentially the ability to synthesize fatty acids with very long chains.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00245154
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