ISSN:
1432-072X
Keywords:
Fatty acids synthetase
;
Very long chain fatty acids
;
Psychrophilic bacteria
;
Vibrio sp
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
Notes:
Abstract The activity of fatty acid synthetase (FAS) from Vibrio sp. strain ABE-1 required the presence of acyl carrier protein and was completely inhibited by thiolactomycin, an inhibitor specific for a type II FAS. These observations indicate that this enzyme is a type II FAS. Analysis by gas-liquid chromotography of the reaction products synthesized in vitro from [2-14C]malonyl-CoA by the partially purified FAS revealed, in addition to 16-and 18-carbon fatty acids which are normal constituents of this bacterium, the presence of fatty acids with very long chains. These fatty acids were identified as saturated and mono-unsaturated fatty acids with 20 up to as many as 30 carbon atoms. The longest fatty acids normally found in this bacterium contain 18-carbon atoms. These results suggest that the FAS from Vibrio sp. strain ABE-1 has potentially the ability to synthesize fatty acids with very long chains.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF00245154
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