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  • 1
    Online Resource
    Online Resource
    Newark :John Wiley & Sons, Incorporated,
    Keywords: Capillary electrophoresis. ; Electronic books.
    Type of Medium: Online Resource
    Pages: 1 online resource (288 pages)
    Edition: 1st ed.
    ISBN: 9780470434918
    Language: English
    Note: Intro -- NANOCHROMATOGRAPHY AND NANOCAPILLARY ELECTROPHORESIS -- CONTENTS -- PREFACE -- ACKNOWLEDGMENTS -- 1 Introduction -- 1.1. Nanoanalyses -- 1.2. Definition of Nanochromatography and Nanocapillary Electrophoresis -- 1.3. Nanochromatography and Nanocapillary Electrophoresis -- 1.4. Fabrication of Microdevices -- 1.5. Developments in Nanoanalyses -- 1.6. Data Integration -- 1.7. Protocol of Nanoanalyses -- 1.8. Scope of the Book -- 1.9. Conclusion -- References -- 2 Fabrication of Microchips -- 2.1. Introduction -- 2.2. Substrates -- 2.3. Techniques of Fabrication -- 2.3.1. Glass Chips -- 2.3.2. Quartz Chips -- 2.3.3. Silica Chips -- 2.3.4. Polymer Chips -- 2.3.5. Plastic Chips -- 2.3.6. Chips and the Polymerase Chain Reaction -- 2.4. Surface Modification -- 2.4.1. Modification by Polymers -- 2.4.2. Modification by Silica Gel -- 2.4.3. Modification by Monolithics -- 2.4.4. Modification by Sulfonation -- 2.5. Designs of Chips -- 2.6. Bindings in Chips -- 2.7. Conclusion -- References -- 3 Instrumentation of Nanochromatography and Nanocapillary Electrophoresis -- 3.1. Introduction -- 3.2. Nanoliquid Chromatography (NLC) -- 3.2.1. Mobile Phase Reservoirs -- 3.2.2. Mobile Phases and Flow Calibration -- 3.2.3. Mobile Phase Tubings -- 3.2.4. Solvent Delivery Pump -- 3.2.5. Sample Injector -- 3.2.6. Separation Chips -- 3.2.7. Detectors -- 3.2.8. Recorder -- 3.2.9. Sample Preparation Units -- 3.3. Nanocapillary Electrophoresis -- 3.3.1. Separation Chip -- 3.3.2. Background Electrolyte and Its Reservoirs -- 3.3.3. Sample Injection Port -- 3.3.4. Detectors -- 3.3.5. Recorder -- 3.3.6. Sample Preparation Units -- 3.4. Conclusion -- References -- 4 Detection in Nanochromatography and Nanocapillary Electrophoresis -- 4.1. Introduction -- 4.2. Mass Spectrometer Detectors -- 4.3. Fluorescence Detectors -- 4.4. Electrochemical Detectors. , 4.5. Element Specific Detectors -- 4.6. Miscellaneous Detectors -- 4.7. Conclusion -- References -- 5 Sample Preparation in Nanochromatography and Nanocapillary Electrophoresis -- 5.1. Introduction -- 5.2. Sample Preparation -- 5.3. Sampling -- 5.3.1. Biological Samples -- 5.3.1.1. Blood -- 5.3.1.2. Urine -- 5.3.1.3. Tissues -- 5.3.1.4. Plants -- 5.3.1.5. Food Stuffs -- 5.3.2. Environmental Samples -- 5.3.2.1. Air -- 5.3.2.2. Water -- 5.3.2.3. Sediment and Soils -- 5.4. Preservation -- 5.5. Filtration -- 5.6. Digestion/Homogenization -- 5.7. Extractions -- 5.8. Clean Up -- 5.9. Preconcentration -- 5.10. Off-Line Nanosample Preparation Methods -- 5.10.1. Nano Solid Phase Extractions -- 5.10.2. Nano Membrane Extractions -- 5.10.3. Nano Miscellaneous Extractions -- 5.11. Online Nanosample Preparation Methods -- 5.12. Conclusion -- References -- 6 Nano-High Performance Liquid Chromatography -- 6.1. Introduction -- 6.2. Nano-HPLC -- 6.3. Applications -- 6.3.1. Nano-HPLC of Biological Matrices -- 6.3.1.1. Proteomics -- 6.3.1.2. Drugs Development and Design -- 6.3.1.3. Environmental Analysis -- 6.4. Optimization of Separations in Nano-HPLC -- 6.5. Troubleshooting in Nano-HPLC -- 6.6. Conclusion -- References -- 7 Nanocapillary Electrochromatography and Nanomicellar Electrokinetic Chromatography -- 7.1. Introduction -- 7.2. Nanocapillary Electrochromatography -- 7.2.1. Biological Samples -- 7.2.2. Environmental Samples -- 7.2.3. Mechanism of Separation -- 7.3. Nanomicellar Electrokinetic Chromatography -- 7.3.1. Biological Samples -- 7.3.2. Environmental Samples -- 7.3.3. Mechanisms of Separation -- 7.4. Conclusion -- References -- 8 Nanocapillary Electrophoresis -- 8.1. Introduction -- 8.2. Optimization -- 8.3. Applications -- 8.3.1. Proteomics -- 8.3.2. Genomics -- 8.3.3. Amino Acids -- 8.3.4. Drug Development and Design -- 8.3.5. Enzymes and Hormones. , 8.3.6. Biological Fluids -- 8.3.7. Foods and Beverages -- 8.3.8. Viruses and Bacteria -- 8.3.9. Explosives -- 8.3.10. Environmental Analyses -- 8.3.11. Miscellaneous Applications -- 8.4. Mechanism of Separation -- 8.5. Conclusion -- References -- 9 Chiral Separations by Nanoliquid Chromatography and Nanocapillary Electrophoresis -- 9.1. Introduction -- 9.2. Nanoliquid Chromatography -- 9.3. Nanocapillary Electrophoresis -- 9.4. Mechanisms of Chiral Separation -- 9.5. Conclusion -- References -- 10 Perspectives on Nanoanalyses -- 10.1. Introduction -- 10.2. Future of Microfluidic Devices -- 10.3. Future Challenges -- 10.4. Conclusion -- References -- Subject Index.
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  • 2
    Keywords: Physical geography. ; Geology. ; Atmospheric science. ; Geochemistry.
    Description / Table of Contents: Section 1. Hydrology, Hydrogeology, Hydrogeochemistry and Water Resources -- Section 2. Biogeochemistry, Geobiology and Geoecology -- Section 3. Atmospheric Sciences, Meteorology, Climatology, Oceanography.
    Type of Medium: Online Resource
    Pages: 1 Online-Ressource(XXX, 293 p. 181 illus., 171 illus. in color.)
    Edition: 1st ed. 2023.
    ISBN: 9783031431692
    Series Statement: Advances in Science, Technology & Innovation, IEREK Interdisciplinary Series for Sustainable Development
    Language: English
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  • 3
    Keywords: Earth sciences. ; Physical geography. ; Environment. ; Environmental monitoring.
    Description / Table of Contents: Section 1. Environmental Earth Sciences -- Section 2. Geomorphology, Landslides, Soil Science, Paleoclimate, Geoarchaeology -- Section 3. Caves and Karst, a special session on the occasion of the International Year of Caves and Karst (2021).
    Type of Medium: Online Resource
    Pages: 1 Online-Ressource(XXXI, 258 p. 150 illus., 137 illus. in color.)
    Edition: 1st ed. 2023.
    ISBN: 9783031429170
    Series Statement: Advances in Science, Technology & Innovation, IEREK Interdisciplinary Series for Sustainable Development
    Language: English
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  • 4
    Electronic Resource
    Electronic Resource
    New York, NY : Wiley-Blackwell
    Biomedical Chromatography 6 (1992), S. 196-197 
    ISSN: 0269-3879
    Keywords: Chemistry ; Analytical Chemistry and Spectroscopy
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Medicine
    Notes: The separation of tetracycline and amino glycopeptide antibiotics was achieved on silica gel thin layers. Tetracycline antibiotics were resolved on a Co+2 (1.0%) impregnated silica gel layer using ethanol:acetic acid: water (10:6:6, v/v/v) as the mobile phase. Amino glycopeptide antibiotics were separated on an untreated silica gel layer using the mobile phase n-butanol: formic acid: water (6:5:7, v/v/v). The spots of these antibiotics were located by exposing the chromatoplate to iodine vapours.
    Additional Material: 2 Tab.
    Type of Medium: Electronic Resource
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  • 5
    Electronic Resource
    Electronic Resource
    Springer
    Chromatographia 23 (1987), S. 207-208 
    ISSN: 1612-1112
    Keywords: Thin-layer chromatography ; Halide ion impregnated silica gel ; Amino acids
    Source: Springer Online Journal Archives 1860-2000
    Topics: Chemistry and Pharmacology
    Notes: Abstract TLC of a fifteen component mixture of amino acids has been carried out in two ways; firstly, the amino acids were treated with halides below their isoelectric points and chromatographed on plain silica plates, and secondly the amino acids in their cationic forms were chromatographed on silica plates impregnated with halides, keeping the same solvent system. The resolution is considered to be affected by hydrophobic interactions between silica gel and amino acid molecule and by the polarity and the flow of the mobile phase. The method provides resolution of 10–11 amino acids from the fifteen component mixture.
    Type of Medium: Electronic Resource
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  • 6
    Electronic Resource
    Electronic Resource
    Springer
    Fresenius' Zeitschrift für analytische Chemie 329 (1988), S. 793-793 
    ISSN: 1618-2650
    Source: Springer Online Journal Archives 1860-2000
    Topics: Chemistry and Pharmacology
    Type of Medium: Electronic Resource
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  • 7
    Publication Date: 2013-07-13
    Description: Background: Cotton leaf curl disease, caused by single-stranded DNA viruses of the genus Begomovirus (family Geminiviridae), is a major constraint to cotton cultivation across Pakistan and north-western India. At this time only cotton varieties with moderate tolerance are available to counter the disease. microRNAs (miRNAs) are a class of endogenous small RNA molecules that play an important role in plant development, signal transduction, and response to biotic and a biotic stress. Studies have shown that miRNAs can be engineered to alter their target specificity. Such artificial miRNAs (amiRNAs) have been shown to provide resistance against plant-infecting viruses. Results: Two amiRNA constructs, based on the sequence of cotton miRNA169a, were produced containing 21 nt of the V2 gene sequence of Cotton leaf curl Burewala virus (CLCuBuV) and transformed into Nicotiana benthamiana. The first amiRNA construct (P1C) maintained the miR169a sequence with the exception of the replaced 21 nt whereas in the second (P1D) the sequence of the miRNA169a backbone was altered to restore some of the hydrogen bonding of the mature miRNA duplex. P1C transgenic plants showed good resistance when challenge with CLCuBV; plants being asymptomatic with low viral DNA levels. The resistance to heterologous viruses was lower and correlated with the numbers of sequence mismatches between the amiRNA and the V2 gene sequence. P1D plants showed overall poorer resistance to challenge with all viruses tested. Conclusions: The results show that the amiRNA approach can deliver efficient resistance in plants against a monopartite begomoviruses and that this has the potential to be broad-spectrum, providing protection from a number of viruses. Additionally the findings indicate that the levels of resistance depend upon the levels of complementarity between the amiRNA and the target sequence and the sequence of the miRNA backbone, consistent with earlier studies.
    Electronic ISSN: 1743-422X
    Topics: Medicine
    Published by BioMed Central
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  • 8
    Publication Date: 2017-06-10
    Description: Current day consumers prefer natural antioxidants to synthetic antioxidants because they are more active. However, the activity generally depends on the specific condition and composition of food. The aim of t...
    Electronic ISSN: 1476-511X
    Topics: Biology
    Published by BioMed Central
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  • 9
    Publication Date: 2018-07-03
    Description: Experimental results are presented on the particulate matter emissions from a fixed bed combustion reactor chamber combusting two types of ambient dried biomass fuels, six different particle sizes (0.044-0.149mm) in seven different temperature zones (600-1200 ºC).The fuel was ground into various particle sizes and the temperature of combustion was varied for investigation on the emission due from the various parameters. Measurements were made for particulate matter (PM) during the whole phase of combustion by a portable soot analyzer. The PM investigated here is total catch that includes solid particles of elemental carbon and fuel and condensed organic compounds due from incomplete combustion which are less than or equal to 10µm in aerodynamic diameter. The fuel was ground by a grinder and sieved into different sizes by a shake sieve with various mesh sizes. It was found that the PM emission from both biomass fuels was significantly dependent on the temperature of combustion wh...
    Print ISSN: 1755-1307
    Electronic ISSN: 1755-1315
    Topics: Geography , Geosciences , Physics
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  • 10
    Publication Date: 2018-06-21
    Description: Sustainability, Vol. 10, Pages 2101: Impact of Climate Smart Agriculture (CSA) Practices on Cotton Production and Livelihood of Farmers in Punjab, Pakistan Sustainability doi: 10.3390/su10062101 Authors: Muhammad Ali Imran Asghar Ali Muhammad Ashfaq Sarfraz Hassan Richard Culas Chunbo Ma Cotton is the second largest crop of Pakistan in terms of area after wheat and is being suffered by multiple shocks over the time due to conventional agricultural management practices, climate change, and market failures. Climate Smart Agriculture (CSA) was introduced by the Food and Agricultural Organization (FAO) in 2010, as an innovative cleaner production alternative to conventional farming that aimed at increasing the efficiency of natural resources, resilience, and productivity of agricultural production system, while reducing greenhouse gas emissions. The adverse effects of climate change on cotton production at the farm and regional level can be minimized by using CSA practices and technologies. The present study investigated the financial performance and explored the impact of CSA through sustainable water use management on cotton production in Lower Bari Doab Canal (LBDC) irrigation system of Punjab, Pakistan by using Cobb-Douglas production function. The adopters of CSA in cotton cultivation were identified by conducting six focus group discussions. Data were collected through well-structured questionnaire from 133 adopters of CSA and 65 conventional cotton growers for the cropping season 2016–2017. It was found that water-smart (raising crops on bed, laser land levelling, conjunctive use of water and drainage management), energy-smart (minimum tillage), carbon-smart (less use of chemicals) and knowledge-smart (crop rotation and improved varieties i.e., tolerant to drought, flood and heat/cold stresses) practices and technologies of CSA were adopted by the cotton farmers in the study area. Most of the farmers were of the view that they are adopting CSA practices and technologies due to the limited supply of canal water, climate change, drought-prone, massive groundwater extraction, rapidly declining groundwater table and increasing soil salinity over the time. Results revealed that uniform germination, higher yield and financial returns, the concentration of inputs and increase in resource use efficiency are the main advantages of CSA. The econometric analysis showed that implementation of CSA practices and technologies as judicious use of water and fertilizer, groundwater quality, access to extension services, and appropriate method and time of picking have a significant impact on the gross value of cotton product (GVP). The findings of the study would be helpful for policy makers to formulate policies that can minimize farmer’s financial burden to adopt CSA technologies and implement for scaling out in Punjab and beyond.
    Electronic ISSN: 2071-1050
    Topics: Energy, Environment Protection, Nuclear Power Engineering
    Published by MDPI Publishing
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