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  • 1
    Digitale Medien
    Digitale Medien
    Minimalist Aminoacylated RNAs as Efficient Substrates for Elongation Factor Tu (1994)
    s.l. : American Chemical Society
    Biochemistry 33 (1994), S. 5682-5688 
    Details
    ISSN: 1520-4995
    Quelle: ACS Legacy Archives
    Thema: Biologie , Chemie und Pharmazie
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1021/bi00185a003
    Standort Signatur Einschränkungen Verfügbarkeit
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    Artikel (Nationallizenzen)
    Volltext
  • 2
    Digitale Medien
    Digitale Medien
    Quantitation of plasmid DNA in aqueous two-phase systems by fluorescence analysis (2000)
    Springer
    Biotechnology letters 22 (2000), S. 1101-1104 
    Details
    ISSN: 1573-6776
    Schlagwort(e): aqueous two-phase systems ; fluorescence ; PicoGreen ; plasmid DNA
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Werkstoffwissenschaften, Fertigungsverfahren, Fertigung
    Notizen: Abstract The development of aqueous two-phase systems for plasmid purification from Escherichia coli cell lysates requires a reliable DNA quantitation method. Plasmid DNA was quantified by fluorescence using PicoGreen nucleic acid stain. Linearity was obtained up to 40 ng plasmid ml−1. Two polyethyleneglycol (PEG)/salt systems were studied, PEG 600/K2HPO4 and PEG 300/K2HPO4. The average plasmid recovery was 41% in the bottom phase of the first system and 35% in the top phase of the second system. This method has proved to be simple and reproducible.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1023/A:1005610113677
    Standort Signatur Einschränkungen Verfügbarkeit
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    Artikel (Nationallizenzen)
    Volltext
  • 3
    facet.materialart.
    Unbekannt
    Dinocyst assemblages in sediment core MSM05/3_343310 from Disko Bugt, West Greenland (2019)
    PANGAEA
    In:  Supplement to: Allan, Estelle; de Vernal, Anne; Knudsen, Mads Faurschou; Hillaire-Marcel, Claude; Moros, Matthias; Ribeiro, Sofia; Ouellet‐Bernier, Marie‐Michèle; Seidenkrantz, Marit-Solveig (2018): Late Holocene sea surface instabilities in the Disko Bugt Area, West Greenland, in phase with δ18O oscillations at Camp Century. Paleoceanography and Paleoclimatology, 33(2), 227-243, https://doi.org/10.1002/2017PA003289
    Details
    Publikationsdatum: 2024-02-02
    Beschreibung: Palynological analyses of sediment core MSM343310 from Disko Bugt (68°38.861' N, 53°49.493' W) document decadal‐ to centennial‐scale variability of sea surface conditions during the last ~3,600 years. Dinocyst fluxes (〉10⁴ cysts/cm² yr⁻¹) indicate a very high productivity. Dinocyst assemblages dominated by Islandinium minutum, Brigantedinium spp., Islandinium? cezare, and the cyst of Pentapharsodinium dalei suggest low surface salinity and marked shifts in summer sea surface temperature. The application of the modern analog technique to dinocyst assemblages, using an updated reference data set with new sites from the West Greenland margin, led to reconstruct decadal‐centennial‐scale variations in sea surface salinity and temperature, in phase with the δ18O variations in the Camp Century ice core. At ~1.5 ka BP, the seasonal sea ice cover records an important regime change, from winter‐only sea ice to more unstable conditions marked by successive cooling pulses with sea ice cover of up to 8 months/yr. The data suggest a close relationship between hydrographic conditions and regional climate over Greenland. Our record shows variations with a mean 200 years periodicity until ~2 ka BP, which supports the hypothesis of climate variations driven by solar variability. After 1.5 ka BP, our data show a variability characterized by a 60-70 year periodicity, which suggests linkages with the Atlantic Multidecadal Oscillation and southwestward migration of the atmospheric polar front. The most recent part of the record, from ~1900 CE to 2007 CE, is characterized by assemblages reflecting warmer surface conditions and reduced sea ice cover.
    Schlagwort(e): Achomosphaera spp.; Age; AGE; Arctic climate change; Arctic sea ice reconstructions; Baffin Bay; Bitectatodinium tepikiense; Brigantedinium spp.; Counting, palynology; Dinocyst database; Dubridinium spp.; Echinidinium spp.; Event label; GC; Gravity corer; Impagidinium aculeatum; Impagidinium pallidum; Islandinium? cezare; Islandinium brevispinosum; Islandinium minutum; Late Holocene; Lejeunecysta spp.; Maria S. Merian; MSM05/3; MSM05/3_343310-2-2; MSM05/3_343310-5-1; MUC; MultiCorer; Nematosphaeropsis labyrinthus; Operculodinium centrocarpum; Pentapharsodinium dalei cyst; Protoperidinium americanum; Protoperidinium nudum cyst; Selenopemphix quanta; Spiniferites belerius; Spiniferites bentori; Spiniferites elongatus; Spiniferites ramosus; Spiniferites spp.; Votadinium calvum; West Greenland
    Materialart: Dataset
    Format: text/tab-separated-values, 5064 data points
    Standort Signatur Einschränkungen Verfügbarkeit
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    DATA PANGAEA
  • 4
    facet.materialart.
    Unbekannt
    Dinocyst percentages in sediment core MSM05/3_343310 from Disko Bugt, West Greenland (2019)
    PANGAEA
    In:  Supplement to: Allan, Estelle; de Vernal, Anne; Knudsen, Mads Faurschou; Hillaire-Marcel, Claude; Moros, Matthias; Ribeiro, Sofia; Ouellet‐Bernier, Marie‐Michèle; Seidenkrantz, Marit-Solveig (2018): Late Holocene sea surface instabilities in the Disko Bugt Area, West Greenland, in phase with δ18O oscillations at Camp Century. Paleoceanography and Paleoclimatology, 33(2), 227-243, https://doi.org/10.1002/2017PA003289
    Details
    Publikationsdatum: 2024-02-02
    Beschreibung: Palynological analyses of sediment core MSM343310 from Disko Bugt (68°38.861' N, 53°49.493' W) document decadal‐ to centennial‐scale variability of sea surface conditions during the last ~3,600 years. Dinocyst fluxes (〉10⁴ cysts/cm² yr⁻¹) indicate a very high productivity. Dinocyst assemblages dominated by Islandinium minutum, Brigantedinium spp., Islandinium? cezare, and the cyst of Pentapharsodinium dalei suggest low surface salinity and marked shifts in summer sea surface temperature. The application of the modern analog technique to dinocyst assemblages, using an updated reference data set with new sites from the West Greenland margin, led to reconstruct decadal‐centennial‐scale variations in sea surface salinity and temperature, in phase with the δ18O variations in the Camp Century ice core. At ~1.5 ka BP, the seasonal sea ice cover records an important regime change, from winter‐only sea ice to more unstable conditions marked by successive cooling pulses with sea ice cover of up to 8 months/yr. The data suggest a close relationship between hydrographic conditions and regional climate over Greenland. Our record shows variations with a mean 200 years periodicity until ~2 ka BP, which supports the hypothesis of climate variations driven by solar variability. After 1.5 ka BP, our data show a variability characterized by a 60-70 year periodicity, which suggests linkages with the Atlantic Multidecadal Oscillation and southwestward migration of the atmospheric polar front. The most recent part of the record, from ~1900 CE to 2007 CE, is characterized by assemblages reflecting warmer surface conditions and reduced sea ice cover.
    Schlagwort(e): Achomosphaera spp.; Age; AGE; Arctic climate change; Arctic sea ice reconstructions; Baffin Bay; Bitectatodinium tepikiense; Brigantedinium spp.; Counting, palynology; Dinocyst database; Dubridinium spp.; Echinidinium spp.; Event label; GC; Gravity corer; Impagidinium aculeatum; Impagidinium pallidum; Islandinium? cezare; Islandinium brevispinosum; Islandinium minutum; Late Holocene; Lejeunecysta spp.; Maria S. Merian; MSM05/3; MSM05/3_343310-2-2; MSM05/3_343310-5-1; MUC; MultiCorer; Nematosphaeropsis labyrinthus; Operculodinium centrocarpum; Pentapharsodinium dalei cyst; Protoperidinium americanum; Protoperidinium nudum cyst; Selenopemphix quanta; Spiniferites belerius; Spiniferites bentori; Spiniferites elongatus; Spiniferites ramosus; Spiniferites spp.; Votadinium calvum; West Greenland
    Materialart: Dataset
    Format: text/tab-separated-values, 5064 data points
    Standort Signatur Einschränkungen Verfügbarkeit
    BibTip Andere fanden auch interessant ...
    DATA PANGAEA
  • 5
    facet.materialart.
    Unbekannt
    Absolute abundances of dinoflagellate cysts in surface sediment samples from the Celtic Sea (2009)
    PANGAEA
    Details
    Publikationsdatum: 2024-02-02
    Schlagwort(e): Celtic_Sea; Center for Marine Environmental Sciences; GeoB; Geosciences, University of Bremen; Laboratory; Lycopodium marker-grain method; MARUM; MUC; MultiCorer; Species
    Materialart: Dataset
    Format: text/tab-separated-values, 2754 data points
    Standort Signatur Einschränkungen Verfügbarkeit
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    DATA PANGAEA
  • 6
    facet.materialart.
    Unbekannt
    Absolute abundances of dinoflagellate cysts in surface sediment samples from the North Sea (2009)
    PANGAEA
    Details
    Publikationsdatum: 2024-02-02
    Schlagwort(e): Center for Marine Environmental Sciences; GeoB; Geosciences, University of Bremen; Laboratory; Lycopodium marker-grain method; MARUM; North_Sea; North Sea; see reference(s); Species
    Materialart: Dataset
    Format: text/tab-separated-values, 1969 data points
    Standort Signatur Einschränkungen Verfügbarkeit
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    DATA PANGAEA
  • 7
    facet.materialart.
    Unbekannt
    Absolute abundances of dinoflagellate cysts in surface sediment samples from NW Africa (2009)
    PANGAEA
    Details
    Publikationsdatum: 2024-02-02
    Schlagwort(e): Center for Marine Environmental Sciences; GeoB; Geosciences, University of Bremen; Laboratory; Lycopodium marker-grain method; MARUM; MUC; MultiCorer; NW_Africa; Species
    Materialart: Dataset
    Format: text/tab-separated-values, 2041 data points
    Standort Signatur Einschränkungen Verfügbarkeit
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    DATA PANGAEA
  • 8
    facet.materialart.
    Unbekannt
    Absolute abundances of dinoflagellate cysts insurface sediment samples from the Benguela site (2009)
    PANGAEA
    Details
    Publikationsdatum: 2024-02-02
    Schlagwort(e): Benguela; Center for Marine Environmental Sciences; GeoB; Geosciences, University of Bremen; Laboratory; Lycopodium marker-grain method; MARUM; Species
    Materialart: Dataset
    Format: text/tab-separated-values, 14353 data points
    Standort Signatur Einschränkungen Verfügbarkeit
    BibTip Andere fanden auch interessant ...
    DATA PANGAEA
  • 9
    facet.materialart.
    Unbekannt
    Absolute abundances of dinoflagellate cysts in surface sediment samples from four sites: Lycopodium marker-grain method (2009)
    PANGAEA
    In:  Supplement to: Mertens, Kenneth Neil; Verhoeven, Koen; Verleye, Thomas; Louwye, Stephen; Amorim, Ana; Ribeiro, Sofia; Deaf, Amr S; Harding, Ian C; De Schepper, Stijn; González, Catalina; Kodrans-Nsiah, Monika; de Vernal, Anne; Henry, Maryse; Radi, Taoufik; Dybkjaer, Karen; Poulsen, Niels E; Feist-Burkhardt, Susanne; Chitolie, Jonah; Heilmann-Clausen, Claus; Londeix, Laurent; Turon, Jean-Louis; Marret, Fabienne; Matthiessen, Jens; McCarthy, Francine M G; Prasad, Vandana; Pospelova, Vera; Hughes, Jane E Kyffin; Riding, James B; Rochon, André; Sangiorgi, Francesca; Welters, Natasja; Sinclair, Natalie; Thun, Christian; Soliman, Ali; Van Nieuwenhove, Nicolas; Vink, Annemiek; Young, Martin (2009): Determining the absolute abundance of dinoflagellate cysts in recent marine sediments: The Lycopodium marker-grain method put to the test. Review of Palaeobotany and Palynology, Review of Palaeobotany and Palynology, 157(3-4), 238-252, https://doi.org/10.1016/j.revpalbo.2009.05.004
    Details
    Publikationsdatum: 2024-02-02
    Beschreibung: Absolute abundances (concentrations) of dinoflagellate cysts are often determined through the addition of Lycopodium clavatum marker-grains as a spike to a sample before palynological processing. An inter-laboratory calibration exercise was set up in order to test the comparability of results obtained in different laboratories, each using its own preparation method. Each of the 23 laboratories received the same amount of homogenized splits of four Quaternary sediment samples. The samples originate from different localities and consisted of a variety of lithologies. Dinoflagellate cysts were extracted and counted, and relative and absolute abundances were calculated. The relative abundances proved to be fairly reproducible, notwithstanding a need for taxonomic calibration. By contrast, excessive loss of Lycopodium spores during sample preparation resulted in non-reproducibility of absolute abundances. Use of oxidation, KOH, warm acids, acetolysis, mesh sizes larger than 15 µm and long ultrasonication (〉 1 min) must be avoided to determine reproducible absolute abundances. The results of this work therefore indicate that the dinoflagellate cyst worker should make a choice between using the proposed standard method which circumvents critical steps, adding Lycopodium tablets at the end of the preparation and using an alternative method.
    Schlagwort(e): Benguela; Celtic_Sea; Center for Marine Environmental Sciences; GeoB; Geosciences, University of Bremen; MARUM; MUC; MultiCorer; North_Sea; North Sea; NW_Africa
    Materialart: Dataset
    Format: application/zip, 4 datasets
    Standort Signatur Einschränkungen Verfügbarkeit
    BibTip Andere fanden auch interessant ...
    DATA PANGAEA
  • 10
    facet.materialart.
    Unbekannt
    Reconstructions of sea-surface parameters from sediment core MSM05/3_343310 from Disko Bugt, West Greenland (2019)
    PANGAEA
    In:  Supplement to: Allan, Estelle; de Vernal, Anne; Knudsen, Mads Faurschou; Hillaire-Marcel, Claude; Moros, Matthias; Ribeiro, Sofia; Ouellet‐Bernier, Marie‐Michèle; Seidenkrantz, Marit-Solveig (2018): Late Holocene sea surface instabilities in the Disko Bugt Area, West Greenland, in phase with δ18O oscillations at Camp Century. Paleoceanography and Paleoclimatology, 33(2), 227-243, https://doi.org/10.1002/2017PA003289
    Details
    Publikationsdatum: 2024-02-02
    Beschreibung: Palynological analyses of sediment core MSM343310 from Disko Bugt (68°38.861' N, 53°49.493' W) document decadal‐ to centennial‐scale variability of sea surface conditions during the last ~3,600 years. Dinocyst fluxes (〉10⁴ cysts/cm² yr⁻¹) indicate a very high productivity. Dinocyst assemblages dominated by Islandinium minutum, Brigantedinium spp., Islandinium? cezare, and the cyst of Pentapharsodinium dalei suggest low surface salinity and marked shifts in summer sea surface temperature. The application of the modern analog technique to dinocyst assemblages, using an updated reference data set with new sites from the West Greenland margin, led to reconstruct decadal‐centennial‐scale variations in sea surface salinity and temperature, in phase with the δ18O variations in the Camp Century ice core. At ~1.5 ka BP, the seasonal sea ice cover records an important regime change, from winter‐only sea ice to more unstable conditions marked by successive cooling pulses with sea ice cover of up to 8 months/yr. The data suggest a close relationship between hydrographic conditions and regional climate over Greenland. Our record shows variations with a mean 200 years periodicity until ~2 ka BP, which supports the hypothesis of climate variations driven by solar variability. After 1.5 ka BP, our data show a variability characterized by a 60-70 year periodicity, which suggests linkages with the Atlantic Multidecadal Oscillation and southwestward migration of the atmospheric polar front. The most recent part of the record, from ~1900 CE to 2007 CE, is characterized by assemblages reflecting warmer surface conditions and reduced sea ice cover.
    Schlagwort(e): Age; AGE; Arctic climate change; Arctic sea ice reconstructions; Baffin Bay; Calculated; Dinocyst database; Event label; GC; Gravity corer; Late Holocene; Maria S. Merian; MSM05/3; MSM05/3_343310-2-2; MSM05/3_343310-5-1; MUC; MultiCorer; Paleoproductivity as carbon; Sea ice cover duration; Sea surface salinity, summer; Sea surface salinity, winter; Sea surface temperature, summer; Sea surface temperature, winter; West Greenland
    Materialart: Dataset
    Format: text/tab-separated-values, 1477 data points
    Standort Signatur Einschränkungen Verfügbarkeit
    BibTip Andere fanden auch interessant ...
    DATA PANGAEA
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