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  • 1
    Electronic Resource
    Electronic Resource
    Arbitrary primed PCR fingerprinting and serotyping of clinical Pseudomonas aeruginosa strains (1997)
    Oxford, UK : Blackwell Publishing Ltd
    FEMS immunology and medical microbiology 17 (1997), S. 0 
    Details
    ISSN: 1574-695X
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology , Medicine
    Notes: Arbitrary primed PCR (AP-PCR) analysis was compared with serotyping as a means of high-resolution typing of Pseudomonas aeruginosa. Seventy-four isolates from 3 different hospitals and 18 reference strains were studied. Serotyping provided good index of discrimination, although eleven isolates could not be serotyped. Genomic DNA was amplified with a single 10 nucleotide primer (sequence 5′-AGG GGT CTT G-3′). The strains were genetically diverse and 61 different AP-PCR profiles of 2–7 bands between 0.3 and 2.4 kb were obtained. AP-PCR profiles were not consistently associated with serotypes, but they clearly subtyped strains of the same serotype. Numerical analysis of AP-PCR patterns defined 7 groups at the 55% similarity level, and identified predominant strains in each hospital. The results show that AP-PCR analysis provides a simple and practical approach to typing P. aeruginosa that is more discriminatory than traditional serotyping scheme. We suggest that maximum discrimination can be achieved by a combination of both methods.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1574-695X.1997.tb00994.x
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    Paper (German National Licenses)
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  • 2
    Electronic Resource
    Electronic Resource
    Comparison of 23S polymerase chain reaction–restriction fragment length polymorphism and amplified fragment length polymorphism techniques as typing systems for thermophilic campylobacters (2002)
    Oxford, UK : Blackwell Publishing Ltd
    FEMS microbiology letters 211 (2002), S. 0 
    Details
    ISSN: 1574-6968
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Notes: In this study, we evaluated the combination of polymerase chain reaction–restriction fragment length polymorphism (PCR–RFLP) and amplified fragment length polymorphism (AFLP) molecular typing techniques for the analysis of thermophilic campylobacter species isolated from clinical and poultry samples. 23S PCR–RFLP analysis performed to fingerprint 69 strains exhibited an excellent level of typability. Eleven different types were defined at 100% linkage level following numerical analysis of band patterns. Differentiation of Campylobacter jejuni and Campylobacter coli at species level was achieved although no significant relationship could be observed between the profiles and the origin of the strains. Simplified AFLP analysis of the isolates disclosed the presence of 66 different banding patterns. The resulting dendrogram showed a high diversity among the strains studied. All the isolates were grouped within eight main types with a 69% homology degree among them. Differentiation at subspecies level was possible but no significant relationship could be observed between the AFLP profiles and the origin of the strains. When used in combination, 23S PCR–RFLP and single-enzyme AFLP methods can be applied to determine taxonomic and epidemiological relationships among thermophilic campylobacters.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1574-6968.2002.tb11209.x
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    Paper (German National Licenses)
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  • 3
    Electronic Resource
    Electronic Resource
    Mitochondrial remnant organelles of Giardia function in iron-sulphur protein maturation (2003)
    [s.l.] : Macmillian Magazines Ltd.
    Nature 426 (2003), S. 172-176 
    Details
    ISSN: 1476-4687
    Source: Nature Archives 1869 - 2009
    Topics: Biology , Chemistry and Pharmacology , Medicine , Natural Sciences in General , Physics
    Notes: [Auszug] Giardia intestinalis (syn. lamblia) is one of the most widespread intestinal protozoan pathogens worldwide, causing hundreds of thousands of cases of diarrhoea each year. Giardia is a member of the diplomonads, often described as an ancient protist group whose primitive nature is suggested by ...
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1038/nature01945
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    Paper (German National Licenses)
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  • 4
    Electronic Resource
    Electronic Resource
    The problem of nonadherence to therapy in the management of duodenal ulcer recurrences (1962)
    Springer
    Digestive diseases and sciences 7 (1962), S. 1047-1060 
    Details
    ISSN: 1573-2568
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary Investigations on 24 patients hospitalized for treatment of duodenal ulcer recurrences at the Massachusetts General Hospital revealed that none considered that he had adhered to the prescribed therapy in its entirety. The factors found operative in nonadherence to therapy were presented under four headings: (1) the patient's attitude toward the diagnosis of ulcer; (2) the patient's attitude toward the recommended treatment; (3) the patient's attitude toward the physician; and (4) the response of guilt in the patient who does not adhere to therapy. Cases were presented illustrating the various components in each category, and methods of handling were described.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF02232981
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    Paper (German National Licenses)
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  • 5
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    Multi-scale drought and ocean–atmosphere variability in monsoon Asia (2016)
    IOP Science
    Details
    Publication Date: 2022-05-25
    Description: © The Author(s), 2015. This article is distributed under the terms of the Creative Commons Attribution License. The definitive version was published in Environmental Research Letters 10 (2015): 074010, doi:10.1088/1748-9326/10/7/074010.
    Description: Spatially extensive and persistent drought episodes have repeatedly influenced human history, including the 'Strange Parallels' drought event in monsoon Asia during the mid-18th century. Here we explore the dynamics of sustained monsoon failure using observed and tree-ring reconstructed drought patterns and a 1300-year pre-industrial community earth system model control run. Both modern observational and climate model drought patterns during years with extremely weakened South Asian monsoon resemble those reconstructed for the Strange Parallels drought. Model analysis reveals that this pattern arises during boreal spring over Southeast Asia, with decreased precipitation and moisture flux, while related summertime climate anomalies are confined to the Indian subcontinent. Years with simulated South Asian drying exhibit canonical El Niño conditions over the Pacific and associated shifts in the Walker circulation. In contrast, multi-year drought periods, resembling those sustained during the Strange Parallels drought, feature anomalous Pacific warming around the dateline, typical of El Niño Modoki events.
    Description: This work was performed with support and funding from the Significant Opportunities in Atmospheric Research and Sciences Program (NSF AGS-1120459), WHOI Academic Programs Office Funds, and NSF AGS-1338734, AGS-1203704, and AGS-1304245.
    Repository Name: Woods Hole Open Access Server
    Type: Article
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  • 6
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    Characterizing the Statistics of a Bunch of Optical Pulses Using a Nonlinear Optical Loop Mirror (2015)
    Hindawi
    Details
    Publication Date: 2015-10-23
    Description: We propose in this work a technique for determining the amplitude distribution of a wave packet containing a large number of short optical pulses with different amplitudes. The technique takes advantage of the fast response of the optical Kerr effect in a fiber nonlinear optical loop mirror (NOLM). Under some assumptions, the statistics of the pulses can be determined from the energy transfer characteristic of the packet through the NOLM, which can be measured with a low-frequency detection setup. The statistical distribution is retrieved numerically by approximating the solution of a system of nonlinear algebraic equations using the least squares method. The technique is demonstrated numerically in the case of a packet of solitons.
    Print ISSN: 1024-123X
    Electronic ISSN: 1563-5147
    Topics: Mathematics , Technology
    Published by Hindawi
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  • 7
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    Sustainability, Vol. 8, Pages 680: Transforming Mature Tourism Resorts into Sustainable Tourism Destinations through Participatory Integrated Approaches: The Case of Puerto de la Cruz (2016)
    MDPI Publishing
    Details
    Publication Date: 2016-07-21
    Description: Transforming mature tourism resorts has evolved toward a greater involvement of public authorities and away from the mere renovation of public spaces. Authorities today are required to lead the reorganization of tourism activities through the development of co-operative networks between all stakeholders involved. In this paper, a participatory integrated approach has been designed and implemented in collaboration with Spanish authorities and the tourism sector to propose a strategy to achieve the renovation of tourism resorts. This methodology was applied to Puerto de la Cruz, the oldest tourism destination in the Canary Islands and a clear paradigm of a consolidated resort. The objective is to define and implement policies to transform Puerto de la Cruz into a more sustainable tourism destination.
    Electronic ISSN: 2071-1050
    Topics: Energy, Environment Protection, Nuclear Power Engineering
    Published by MDPI Publishing
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  • 8
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    Sensors, Vol. 17, Pages 147: Continuous Space Estimation: Increasing WiFi-Based Indoor Localization Resolution without Increasing the Site-Survey Effort (2017)
    MDPI Publishing
    In: Sensors
    Details
    Publication Date: 2017-01-14
    Description: Although much research has taken place in WiFi indoor localization systems, their accuracy can still be improved. When designing this kind of system, fingerprint-based methods are a common choice. The problem with fingerprint-based methods comes with the need of site surveying the environment, which is effort consuming. In this work, we propose an approach, based on support vector regression, to estimate the received signal strength at non-site-surveyed positions of the environment. Experiments, performed in a real environment, show that the proposed method could be used to improve the resolution of fingerprint-based indoor WiFi localization systems without increasing the site survey effort.
    Electronic ISSN: 1424-8220
    Topics: Chemistry and Pharmacology , Electrical Engineering, Measurement and Control Technology
    Published by MDPI Publishing
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  • 9
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    Concerted expression of the thermogenic and bioenergetic mitochondrial protein machinery in brown adipose tissue (2013)
    Wiley-Blackwell
    Details
    Publication Date: 2013-04-21
    Description: Brown adipose tissue (BAT) is specialized in non-shivering thermogenesis through the expression of the mitochondrial uncoupling protein-1 (UCP1). In this paper, we describe the relationship between UCP1 and proteins involved in ATP synthesis. By the use of BATIRKO mice, which have enhanced UCP1 expression in BAT, an increase in ATP synthase as well as in ubiquinol cytochrome c reductase levels was observed. Alterations in mitochondrial mass or variations in ATP levels were not observed in BAT of these mice. In addition, using a protocol of brown adipocyte differentiation, the concerted expression of UCP1 with ATP synthase was found. These two scenarios revealed that increases in the uncoupling machinery of brown adypocites must be concomitantly followed by an enhancement of proteins involved in ATP synthesis. These concerted changes reflect the need to maintain ATP production in an essentially uncoupling cell type. J. Cell. Biochem. © 2013 Wiley Periodicals, Inc.
    Electronic ISSN: 0091-7419
    Topics: Biology , Chemistry and Pharmacology , Medicine
    Published by Wiley-Blackwell
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  • 10
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    Sustainability, Vol. 9, Pages 1346: Cultural Heritage and Urban Tourism: Historic City Centres under Pressure (2017)
    MDPI Publishing
    Details
    Publication Date: 2017-08-05
    Description: Sustainability, Vol. 9, Pages 1346: Cultural Heritage and Urban Tourism: Historic City Centres under Pressure Sustainability doi: 10.3390/su9081346 Authors: María García-Hernández Manuel de la Calle-Vaquero Claudia Yubero Historic city centres of European cities are one of the most important elements of the European cultural heritage. They are places that attract many visitors due to their relevance in terms of heritage, but the recent growth of tourist flows constitutes a threat to the conservation of their values. In some European cities, such as Venice or Barcelona, the debate has taken to the streets, and there is significant social mobilization taking place, with very belligerent positions against tourism (anti-tourism, tourismphobia). The mass media also generates discourse on the topic and places the debate on urban tourism sustainability at the forefront of the public debate. In this context, this article reviews the state of the art on tourism impact and identifies, describes and evaluates the different dimensions of tourist pressure based on a case study: the historic centre of the city of Donostia-San Sebastián (Basque Country, Spain). The main goal of the research is to help determine how tourist pressure affects the safeguarding of “historic urban landscapes” and the desirable or desired models of city and tourist destination.
    Electronic ISSN: 2071-1050
    Topics: Energy, Environment Protection, Nuclear Power Engineering
    Published by MDPI Publishing
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