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  • 1980-1984  (3)
Publikationsart
Verlag/Herausgeber
Erscheinungszeitraum
Jahr
  • 1
    Digitale Medien
    Digitale Medien
    Springer
    Applied microbiology and biotechnology 17 (1983), S. 281-286 
    ISSN: 1432-0614
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie , Werkstoffwissenschaften, Fertigungsverfahren, Fertigung
    Notizen: Summary Candida wickerhamii produced ethanol under aerated and nonaerated conditions when grown on glucose but only under non-aerated conditions when grown on cellobiose. When the yeast was grown on 20 g·l−1 glucose in fermentation flasks, the substrate was completely utilized and 9.2 g·l−1 ethanol was produced. When 100 g·l−1 glucose was used, only 60% of the substrate was consumed and 23.4 g·l−1 ethanol was produced fermentatively whereas 31 g·l−1 ethanol was produced in an aerated fermenter. Ethanol toxicity was confirmed by adding ethanol to the culture. No ethanol was produced at added ethanol concentrations of 24 g·l−1 or higher although growth occurred even in the presence of 74 g·l−1 ethanol. The fermentation of glucose and cellobiose (20 g·l−1) was completed in 24 h and 125 h with specific growth rates of 0.29 and 0.06 h−1 respectively. β-Glucosidase was produced when grown on either glucose or cellobiose but the differential rate of enzyme production was 64 fold higher on cellobiose. Increased aeration stimulated enzyme production. β-Glucosidase was present in the fermentation broth and associated with the cells under non-aerated conditions and almost exclusively cell-associated under aerated conditions.
    Materialart: Digitale Medien
    Standort Signatur Einschränkungen Verfügbarkeit
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  • 2
    ISSN: 1432-0614
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie , Werkstoffwissenschaften, Fertigungsverfahren, Fertigung
    Notizen: Summary A defined growth medium was determined for the cellobiose fermenting yeast Candida wickerhamii. Biotin and calcium pantothenate were required for growth while thiamine stimulated growth and ethanol production. The optimum temperature and pH for growth and ethanol production were 30°C and between pH 3 and pH 5 respectively. Oxygen availability played an important role in the fermentation of cellobiose and glucose. The optimum oxygen transfer rate for maximum ethanol production from glucose was 1.75 mmol (g dry biomass · h)−1.
    Materialart: Digitale Medien
    Standort Signatur Einschränkungen Verfügbarkeit
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  • 3
    Digitale Medien
    Digitale Medien
    Springer
    Applied microbiology and biotechnology 18 (1983), S. 369-373 
    ISSN: 1432-0614
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie , Werkstoffwissenschaften, Fertigungsverfahren, Fertigung
    Notizen: Summary Glucose and cellobiose are the principle sugars released during cellulose hydrolysis. The utilization of mixtures of these sugars (10 g·l−1) by Candiada wickerhamii in batch cultures under aerobic and non-aerated conditions was investigated. Glucose was utilized first followed by cellobiose after a diauxic lag. Ethanol was produced from glucose under both aerobic and non-aerated conditions. Following glucose depletion under aerobic conditions, ethanol (produced during growth on glucose) and cellobiose were utilized simultaneously. Under non-aerated conditions ethanol was produced from cellobiose after the diauxic lag. When the glucose concentration was increased from 2 to 8 g·l−1 in glucose: cellobiose mixtures (total sugar 10 g·l−1), decreases of 66–91% were observed in the specific rates of growth, ethanol production, β-glucosidase production and sugar utilization on cellobiose as well as an increase in the diauxic time lag under aerobic and non-aerated conditions. After depletion of glucose, the viability of C. wickerhamii decreased during cellobiose utilization.
    Materialart: Digitale Medien
    Standort Signatur Einschränkungen Verfügbarkeit
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