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  • 1
    Digitale Medien
    Digitale Medien
    Oxford, UK : Blackwell Publishing Ltd
    Journal of food science 29 (1964), S. 0 
    ISSN: 1750-3841
    Quelle: Blackwell Publishing Journal Backfiles 1879-2005
    Thema: Land- und Forstwirtschaft, Gartenbau, Fischereiwirtschaft, Hauswirtschaft , Werkstoffwissenschaften, Fertigungsverfahren, Fertigung
    Notizen: The heat resistance of Salmonella typhimurium in liquid whole egg at pH 5.5 and 55°C was reduced significantly by a number of chemical additives. Of these, the most effective were β-propiolactone, ethylene oxide, and butadiene dioxide. Additional advantages of ethylene oxide and β-propiolactone were the elimination of S. typhimurium from heavily inoculated whole-egg slurry during storage at 0°C and a markedly increased lag phase of growth of the test organism in egg incubated at 30°C. Butadiene dioxide, although not tested at 0°C, was most effective in reducing heat resistance at pH 5.5 and 55° C, and also greatly increased the lag phase of the test organism at 30° C. Unfortunately, this latter compound is carcinogenic to mice.Acetic or lactic acid, used to lower the pH of liquid whole eggs, decreased the heat resistance of S. typhimurium more effectively than did hydrochloric acid, especially at pH 5.5 arid 6.0. Formic and propionic acids also were more effective than HCl when all were tested at pH 5.5. Differences in degree of dissociation between hydrochloric and the organic acids are thought to account, in large measure, for these observations.
    Materialart: Digitale Medien
    Standort Signatur Einschränkungen Verfügbarkeit
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  • 2
    Digitale Medien
    Digitale Medien
    Springer
    Applied microbiology and biotechnology 26 (1987), S. 55-60 
    ISSN: 1432-0614
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie , Werkstoffwissenschaften, Fertigungsverfahren, Fertigung
    Notizen: Summary The cellular long-chain fatty acids of 69 strains of yeasts, representing 29 species associated with the brewing industry, were extracted by saponification and analyzed asmethyl esters by gas chromatography. The strains were characterized by the presence of palmitic, palmitoleic, stearic, oleic, linoleic and linolenic acid as the major fatty acids. The strains were divided into six groups on the basis of their fatty acid content. With this method it was possible to differentiate between the yeasts on species and, in some instances, on strain level.
    Materialart: Digitale Medien
    Standort Signatur Einschränkungen Verfügbarkeit
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  • 3
    Digitale Medien
    Digitale Medien
    Springer
    Applied microbiology and biotechnology 21 (1985), S. 148-153 
    ISSN: 1432-0614
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie , Werkstoffwissenschaften, Fertigungsverfahren, Fertigung
    Notizen: Summary Candida wickerhamii growing on cellobiose produced β-glucosidase with high activity against φ-nitrophenyl glucoside (PNPG) but low activity against cellobiose. β-glucosidase production was constitutive, and was repressed by β-glucosides and glucose. β-glucosides containing an aromatic moiety in the aglycon were the best substrates for β-glucosidase indicating that the enzyme is an aryl-β-glucosidase. A β-glucosidase from C. wickerhamii cells was purified by (NH4)2SO4 precipitation, dialysis, ion-exchange chromatography and gel filtration. The purified enzyme was homogeneous as shown by sodium-dodecyl-sulphate polyacrylamide gel electrophoresis and discontinuous gel electrophoresis. The purified enzyme hydrolysed PNPG but not cellobiose. The Km of the enzyme was 0.185 mM. Glucose inhibited the enzyme competitively and the Ki was 7.5 mM. The apparent molecular mass was 97,000. The optimum pH and temperature for enzyme activity were between pH 7 and 7.4 and 40°C respectively. At temperatures of 45°C and greater the enzyme was inactivated. The activation energy of the enzyme was 29.4 kJ · mol-1.
    Materialart: Digitale Medien
    Standort Signatur Einschränkungen Verfügbarkeit
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  • 4
    Digitale Medien
    Digitale Medien
    Springer
    Applied microbiology and biotechnology 18 (1983), S. 369-373 
    ISSN: 1432-0614
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie , Werkstoffwissenschaften, Fertigungsverfahren, Fertigung
    Notizen: Summary Glucose and cellobiose are the principle sugars released during cellulose hydrolysis. The utilization of mixtures of these sugars (10 g·l−1) by Candiada wickerhamii in batch cultures under aerobic and non-aerated conditions was investigated. Glucose was utilized first followed by cellobiose after a diauxic lag. Ethanol was produced from glucose under both aerobic and non-aerated conditions. Following glucose depletion under aerobic conditions, ethanol (produced during growth on glucose) and cellobiose were utilized simultaneously. Under non-aerated conditions ethanol was produced from cellobiose after the diauxic lag. When the glucose concentration was increased from 2 to 8 g·l−1 in glucose: cellobiose mixtures (total sugar 10 g·l−1), decreases of 66–91% were observed in the specific rates of growth, ethanol production, β-glucosidase production and sugar utilization on cellobiose as well as an increase in the diauxic time lag under aerobic and non-aerated conditions. After depletion of glucose, the viability of C. wickerhamii decreased during cellobiose utilization.
    Materialart: Digitale Medien
    Standort Signatur Einschränkungen Verfügbarkeit
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  • 5
    Digitale Medien
    Digitale Medien
    Springer
    Applied microbiology and biotechnology 23 (1986), S. 499-501 
    ISSN: 1432-0614
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie , Werkstoffwissenschaften, Fertigungsverfahren, Fertigung
    Notizen: Summary Long-chain fatty acids of 8 yeast strains representing 5 species of the genusSaccharomyces, were extracted from yeast cells by saponification and analyzed as methyl esters by gasliquid chromatography (GLC). When these species were grown under standard conditions, each produced a distinctive mean fatty-acid “fingerprint” characterized by certain fatty acid compositions. With this method, it was possible to distinguish between the 5 species within 4 h after they were obtained from 48 h cultures as compared with 7 to 10 days for more conventional methods. Factors such as speed and sensitivity of this method make it an attractive alternative to conventional laboratory tests for distinguishing between species ofSaccharomyces.
    Materialart: Digitale Medien
    Standort Signatur Einschränkungen Verfügbarkeit
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