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  • 1
    Publication Date: 2024-03-22
    Description: We simulated an experimental summer storm in large-volume (~1200 m3, ~16m depth) enclosures in Lake Stechlin by mixing deeper water masses from the meta- and hypolimnion into the mixed layer (epilimnion). The mixing included the disturbance of a deep chlorophyll maximum (DCM) which was present at the same time of the experiment in Lake Stechlin and situated in the metalimnion of each enclosure during filling. Water physical variables and water chemistry was monitored for 42 days after the experimental disturbance event. Mixing disrupted the thermal stratification, increasing concentrations of dissolved nutrients and CO2 and changing light conditions in the epilimnion. Mixing, thus, stimulated phytoplankton growth, resulting in higher particulate matter concentrations of carbon, nitrogen and phosphorous.
    Keywords: Alkalinity, total; Ammonium; Ammonium molybdate reaction (FIASTAR 5000), (0.3 µm GF-75 prefiltered (Sterlitech)); BIBS; Bicarbonate ion; Bridging in Biodiversity Science; Calcium; Calcium carbonate; Calculated using seacarb after Nisumaa et al. (2010); Carbon, inorganic, particulate; Carbon, organic, dissolved; Carbon, organic, particulate; Carbon, total, particulate; Carbonate ion; Carbon dioxide; Carbon dioxide, partial pressure; Chloride; Climate change; Climate driven Changes in Biodiversity of Microbiota; Conductivity, electrolytic; cyanobacteria; DATE/TIME; Day of experiment; DCM; DEPTH, water; Depth of Secchi Disk; Description; Difference derived from TPC and PIC; disturbance; ELTRA-800 (0.3 µm GF-75 filters (Sterlitech)); enclosure; Enclosure experiment; Flow-injection analyzer; Flow-injection analyzer (0.3 µm GF-75 prefiltered (Sterlitech)); Fugacity of carbon dioxide in seawater; Germany; High temperature combustion, infra-red detection (Shimadzu), (0.3 µm GF-75 prefiltered (Sterlitech)); Infralyt 50 (SAXON Junkalor GmbH), (0.3 µm GF-75 filters (Sterlitech)); Ion chromatography (Dionex), (0.3 µm GF-75 prefiltered (Sterlitech)); lake; Lake_Stechlin; Magnesium; mesocosm; Mesocosm label; Nitrate; Nitrite; Nitrogen, total; Nitrogen, total, particulate; Nitrogen, total dissolved; NITROLIMIT; Oxygen; Oxygen/Nitrogen ratio; Oxygen saturation; Oxygen sensor, YSI6560; PAR sensor Li-193SA, LI-COR Inc.; Peroxodisulfate oxidation method; Flow-injection analyzer; 0.3 µm GF-75 filters (Sterlitech); pH; Phosphorus, reactive soluble; Phosphorus, total; Phosphorus, total, particulate; Potassium; Pressure, technical; Radiation, photosynthetically active; SD; Secchi disk; Silicate, dissolved; Sodium; Stickstofflimitation in Binnengewässern; Sulfate; summer storm; TemBi; Temperature, air; Temperature, water; Titration; Treatment; Vaisala Weather station WXT520; water chemistry; water physics
    Type: Dataset
    Format: text/tab-separated-values, 10528 data points
    Location Call Number Limitation Availability
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  • 2
    Publication Date: 2024-03-08
    Description: We simulated an experimental summer storm in large-volume (~1200 m3, ~16m depth) enclosures in Lake Stechlin (https://www.lake-lab.de) by mixing deeper water masses from the meta- and hypolimnion into the mixed layer (epilimnion). The mixing included the disturbance of a deep chlorophyll maximum (DCM) which was present at the same time of the experiment in Lake Stechlin and situated in the metalimnion of each enclosure during filling. Size-fractionated Bacterial Protein Production (BPP) of particle associated (PA, 〉3.0 µm) and free-living bacteria (FL, 0.2-3.0 µm) (14C-Leu incorporation) as well as abundances of PA (microscopy of DAPI stained cells on 3.0 µm polycarbonate filters) and FL heterotrophic prokaryotes and picocyanobacteria (flow cytometry of SYBR green I stained cells) were monitored for 42 days after the experimental disturbance event. Mixing increased bacterial abundance and production about 3 weeks after mixing, which was associated to a mixing-induced stimulation of phytoplankton growth in the mixed enclosures compared to the controls. Simultaneously, decreased abundances of picocyanobacteria could be observed in mixed enclosures.
    Keywords: 14C-leucine incorporation; Bacterial abundance of HDNA-Bacteria; Bacterial abundance of LDNA-Bacteria; bacterial production; BIBS; Bridging in Biodiversity Science; chlorophyll; Climate change; Climate driven Changes in Biodiversity of Microbiota; cyanobacteria; DATE/TIME; Day of experiment; DCM; DEPTH, water; derived; Description; disturbance; enclosure experiment; Enclosure experiment; Epifluorescence microscopy after DAPI staining; Flow cytometry; Free-living bacterial abundance; Germany; lake; Lake_Stechlin; mesocosm experiment; Mesocosm label; NITROLIMIT; Nostocales biovolume; Nostocales cell abundance; Oxygen/Nitrogen ratio; Particle-associated bacterial abundance; Picocyanobacteria abundance; Protein production, free-living bacteria; Protein production, particle associated bacteria; Stickstofflimitation in Binnengewässern; summer storm; TemBi; Treatment
    Type: Dataset
    Format: text/tab-separated-values, 2003 data points
    Location Call Number Limitation Availability
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  • 3
    Publication Date: 2024-03-08
    Description: We simulated an experimental summer storm in large-volume (~1200 m3, ~16m depth) enclosures in Lake Stechlin (https://www.lake-lab.de) by mixing deeper water masses from the meta- and hypolimnion into the mixed layer (epilimnion). The mixing included the disturbance of a deep chlorophyll maximum (DCM) which was present at the same time of the experiment in Lake Stechlin and situated in the metalimnion of each enclosure during filling. Primary production rates as well as exoenzyme activities (alkaline phosphatase, beta-glucosidase, leucine aminopeptidase) were monitored for 42 days after the experimental disturbance event by incubation of size-fractionated sample with H14CO3- and MUF substrate analogue assays, respectively. Mixing disrupted the thermal stratification, increased concentrations of dissolved nutrients and CO2 and changed light conditions in the epilimnion. Thus, mixing stimulated phytoplankton production, resulting in higher primary production rates within one week after mixing.
    Keywords: BIBS; Bridging in Biodiversity Science; Climate change; Climate driven Changes in Biodiversity of Microbiota; cyanobacteria; DATE/TIME; Day of experiment; deep chlorophyll maximum (DCM); DEPTH, water; Description; disturbance; enclosure experiment; Enclosure experiment; Germany; Incubation of size-fractionated sample with H14CO3-; Kinetic constants-maximum enzyme velocity of alkaline phosphatase exoenzyme activity; Kinetic constants-maximum enzyme velocity of alkaline phosphatase potential exoenzyme activity; Kinetic constants-maximum enzyme velocity of leucine aminopeptidase potential exoenzyme activity; lake; Lake_Stechlin; Mesocosm label; mesocosm study; Michealis-Menten constant of alkaline phosphatase potential exoenzyme activity; Michealis-Menten constant of beta-glucosidase potential exoenzyme activity; Michealis-Menten constant of leucine aminopeptidase potential exoenzyme activity; MUF substrate analogue assay; NITROLIMIT; Oxygen/Nitrogen ratio; primary production; Primary production of carbon; Sample incubation with H14CO3-; Stickstofflimitation in Binnengewässern; TemBi; Treatment
    Type: Dataset
    Format: text/tab-separated-values, 2903 data points
    Location Call Number Limitation Availability
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