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  • 1
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    TemBi 2014 mesocosm study: Summer storm impact on primary production rates and enzyme activities in Lake Stechlin (2021)
    PANGAEA
    Details
    Publication Date: 2024-03-08
    Description: We simulated an experimental summer storm in large-volume (~1200 m3, ~16m depth) enclosures in Lake Stechlin (https://www.lake-lab.de) by mixing deeper water masses from the meta- and hypolimnion into the mixed layer (epilimnion). The mixing included the disturbance of a deep chlorophyll maximum (DCM) which was present at the same time of the experiment in Lake Stechlin and situated in the metalimnion of each enclosure during filling. Primary production rates as well as exoenzyme activities (alkaline phosphatase, beta-glucosidase, leucine aminopeptidase) were monitored for 42 days after the experimental disturbance event by incubation of size-fractionated sample with H14CO3- and MUF substrate analogue assays, respectively. Mixing disrupted the thermal stratification, increased concentrations of dissolved nutrients and CO2 and changed light conditions in the epilimnion. Thus, mixing stimulated phytoplankton production, resulting in higher primary production rates within one week after mixing.
    Keywords: BIBS; Bridging in Biodiversity Science; Climate change; Climate driven Changes in Biodiversity of Microbiota; cyanobacteria; DATE/TIME; Day of experiment; deep chlorophyll maximum (DCM); DEPTH, water; Description; disturbance; enclosure experiment; Enclosure experiment; Germany; Incubation of size-fractionated sample with H14CO3-; Kinetic constants-maximum enzyme velocity of alkaline phosphatase exoenzyme activity; Kinetic constants-maximum enzyme velocity of alkaline phosphatase potential exoenzyme activity; Kinetic constants-maximum enzyme velocity of leucine aminopeptidase potential exoenzyme activity; lake; Lake_Stechlin; Mesocosm label; mesocosm study; Michealis-Menten constant of alkaline phosphatase potential exoenzyme activity; Michealis-Menten constant of beta-glucosidase potential exoenzyme activity; Michealis-Menten constant of leucine aminopeptidase potential exoenzyme activity; MUF substrate analogue assay; NITROLIMIT; Oxygen/Nitrogen ratio; primary production; Primary production of carbon; Sample incubation with H14CO3-; Stickstofflimitation in Binnengewässern; TemBi; Treatment
    Type: Dataset
    Format: text/tab-separated-values, 2903 data points
    Location Call Number Limitation Availability
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    DATA PANGAEA
  • 2
    Electronic Resource
    Electronic Resource
    Expression of Opioid Receptor Subtypes and Their Ligands in Fibrillating Human Atria (2005)
    350 Main Street , Malden , MA 02148-5018 , USA and 9600 Garsington Road , Oxford OX4 2DQ , UK . : Blackwell Science Inc
    Pacing and clinical electrophysiology 28 (2005), S. 0 
    Details
    ISSN: 1540-8159
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Medicine
    Notes: The δ- and κ-receptor subtypes are both abundantly expressed in the human heart and participate in age- and stress-related alterations of cardiac function. Opioid receptor agonists mediate cardioprotection in response to ischemic preconditioning via increased intracellular Ca2+ levels, opening mitochondrial KATP channels, and PKC activation. We studied the expression of opioid receptor subtypes κ and δ, and of their ligand precursors, proopiomelanocortin (POMC) and preproenkephalin A (PENKA), in human atrial tissue of patients in sinus rhythm (SR), or persistent atrial fibrillation (AF). The mitochondrial size was also compared between the two groups. The atrial mRNA expression of opioid peptide precursors and receptors was assessed by competitive and real-time RT-PCR in 16 patients in AF and 16 patients in SR. Mitochondria were analyzed in the atrial tissue by electron microscopy in four patients in AF and four patients in SR. Both PENKA (SR: 100 ± 33% vs AF: 33 ± 21%; P 〈 0.05) and κ-receptor mRNA amounts (AF: 78 ± 20% vs SR: 100 ± 11%; P 〈 0.05) were both decreased in AF in comparison to SR. In addition, POMC mRNA levels were decreased in AF (SR: 100 ± 54% vs AF: 37 ± 26%; P 〈 0.05), whereas the expression of the corresponding δ-opioid receptor was unchanged (AF: 102 ± 34% vs 100 ± 44%). Mitochondrial size was increased during persistent AF. Persistent AF is associated with the down-regulation of the opioid receptor/ligand expression. This suggests a loss of protective capacity in the fibrillating atrial tissue, resulting in an ultrastructural remodeling of atrial myocytes.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1540-8159.2005.00073.x
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    Paper (German National Licenses)
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