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  • 1
    ISSN: 1476-4687
    Source: Nature Archives 1869 - 2009
    Topics: Biology , Chemistry and Pharmacology , Medicine , Natural Sciences in General , Physics
    Notes: [Auszug] Marine fixation of atmospheric nitrogen is believed to be an important source of biologically useful nitrogen to ocean surface waters, stimulating productivity of phytoplankton and so influencing the global carbon cycle. The majority of nitrogen fixation in tropical waters is carried out by the ...
    Type of Medium: Electronic Resource
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  • 2
    Publication Date: 2021-02-08
    Description: In vivo confocal Raman microscopy (CRM), polarized light microscopy and Fourier transform infrared spectroscopy (FTIR) were used to determine if a significant amount of amorphous calcium carbonate (ACC) exists within larval shells of Baltic mytilid mussels (Mytilus edulis-like) and whether the amount of ACC varies during larval development. No evidence for ACC was found from the onset of shell deposition at 21 h post-fertilization (hpf) until 48 hpf. Larval Mytilus shells were crystalline from 21 hpf onwards and exhibited CRM and FTIR peaks characteristic of aragonite. Prior to shell deposition at 21 hpf, no evidence for carbonates was observed through in vivo CRM.We further analysed the composition of larval shells in three other bivalve species, Mercenaria mercenaria, Crassostrea gigas and Crassostrea virginica and observed no evidence for ACC, which is in contrast to previous work on the same species. Our findings indicate that larval bivalve shells are composed of crystalline aragonite and we demonstrate that conflicting results are related to sub-optimal measurements and misinterpretation of CRM spectra. Our results demonstrate that the common perception that ACC generally occurs as a stable and abundant precursor during larval bivalve calcification needs to be critically reviewed.
    Type: Article , PeerReviewed , info:eu-repo/semantics/article
    Format: text
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  • 3
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    PANGAEA
    In:  Supplement to: Ramesh, Kirti; Melzner, Frank; Griffith, Andrew W; Gobler, Christopher J; Rouger, Caroline; Tasdemir, Deniz; Nehrke, Gernot (2018): In vivo characterization of bivalve larval shells: a confocal Raman microscopy study. Journal of The Royal Society Interface, 15(141), 20170723, https://doi.org/10.1098/rsif.2017.0723
    Publication Date: 2023-06-24
    Description: In vivo confocal Raman microscopy (CRM), polarized light microscopy and Fourier transform infrared spectroscopy (FTIR) were used to determine if a significant amount of amorphous calcium carbonate (ACC) exists within larval shells of Baltic mytilid mussels (Mytilus edulis-like) and whether the amount of ACC varies during larval development. No evidence for ACC was found from the onset of shell deposition at 21 h post-fertilization (hpf) until 48 hpf. Larval Mytilus shells were crystalline from 21 hpf onwards and exhibited CRM and FTIR peaks characteristic of aragonite. Prior to shell deposition at 21 hpf, no evidence for carbonates was observed through in vivo CRM. We further analysed the composition of larval shells in three other bivalve species, Mercenaria mercenaria, Crassostrea gigas and Crassostrea virginica and observed no evidence for ACC, which is in contrast to previous work on the same species. Our findings indicate that larval bivalve shells are composed of crystalline aragonite and we demonstrate that conflicting results are related to sub-optimal measurements and misinterpretation of CRM spectra. Our results demonstrate that the common perception that ACC generally occurs as a stable and abundant precursor during larval bivalve calcification needs to be critically reviewed.
    Keywords: Analytical method; Sample code/label; Species; Transmission of light; Wave number
    Type: Dataset
    Format: text/tab-separated-values, 143020 data points
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  • 4
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    In:  Supplement to: Talmage, Stephanie C; Gobler, Christopher J (2009): The effects of elevated carbon dioxide concentrations on the metamorphosis, size, and survival of larval hard clams (Mercenaria mercenaria), bay scallops (Argopecten irradians), and Eastern oysters (Crassostrea virginica). Limnology and Oceanography, 54(6), 2072-2080, https://doi.org/10.4319/lo.2009.54.6.2072
    Publication Date: 2024-03-15
    Description: We present experiments that examined the metamorphosis, growth, and survivorship of larvae from three species of commercially and ecologically valuable shellfish (Mercenaria mercenaria, Argopecten irradians, and Crassostrea virginica) at the levels of CO2 projected to occur during the 21st century and beyond. Under CO2 concentrations estimated to occur later this century (~66 Pa, 650 ppm), M. mercenaria and A. irradians larvae exhibited dramatic declines (〉50%) in survivorship as well as significantly delayed metamorphosis and significantly smaller sizes. Although C. virginica larvae also experienced lowered growth and delayed metamorphosis at ~66 Pa CO2, their survival was only diminished at ~152 Pa CO2. The extreme sensitivity of larval stages of shellfish to enhanced levels of CO2 indicates that current and future increases in pelagic CO2 concentrations may deplete or alter the composition of shellfish populations in coastal ecosystems.
    Keywords: Alkalinity, total; Alkalinity, total, standard deviation; Animalia; Aragonite saturation state; Aragonite saturation state, standard deviation; Argopecten irradians; Bicarbonate ion; Bottles or small containers/Aquaria (〈20 L); Calcite saturation state; Calcite saturation state, standard deviation; Calculated using CO2SYS; Calculated using seacarb after Nisumaa et al. (2010); Carbon, inorganic, dissolved; Carbon, inorganic, dissolved, standard deviation; Carbonate ion; Carbonate system computation flag; Carbon dioxide; Carbon dioxide, partial pressure; Carbon dioxide, partial pressure, standard deviation; Carbon dioxide, standard deviation; Crassostrea virginica; Development; EGM-4 Environmental Gas Analyzer (PP Systems) using a Liqui-CelH Membrane (Membr; EPOCA; EUR-OCEANS; European network of excellence for Ocean Ecosystems Analysis; European Project on Ocean Acidification; Experimental treatment; Experiment day; Fugacity of carbon dioxide (water) at sea surface temperature (wet air); Laboratory experiment; Mercenaria mercenaria; Metamorphosed, larvae; Mollusca; Mortality; Mortality/Survival; North Atlantic; OA-ICC; Ocean Acidification International Coordination Centre; Partial pressure of carbon dioxide (water) at sea surface temperature (wet air); Pediveliger, larvae; Pelagos; pH; pH, standard deviation; pH meter; Salinity; Salinity, standard deviation; Single species; Species; Survival; Temperature, standard deviation; Temperature, water; Veliger, larvae; Zooplankton
    Type: Dataset
    Format: text/tab-separated-values, 2461 data points
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  • 5
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    In:  Supplement to: Hattenrath-Lehmann, Theresa K; Smith, Juliette L; Wallace, Ryan B; Merlo, Lucas R; Koch, Florian; Mittelsdorf, Heidi; Goleski, Jennifer A; Anderson, Donald M; Gobler, Christopher J (2015): The effects of elevated CO2 on the growth and toxicity of field populations and cultures of the saxitoxin-producing dinoflagellate, Alexandrium fundyense. Limnology and Oceanography, 60(1), 198-214, https://doi.org/10.1002/lno.10012
    Publication Date: 2024-03-15
    Description: The effects of coastal acidification on the growth and toxicity of the saxitoxin-producing dinoflagellate Alexandrium fundyense were examined in culture and ecosystem studies. In culture experiments, Alexandrium strains isolated from Northport Bay, New York, and the Bay of Fundy, Canada, grew significantly faster (16-190%; p 〈 0.05) when exposed to elevated levels of PCO2 ( 90-190 Pa=900-1900 µatm) compared to lower levels ( 40 Pa=400 µatm). Exposure to higher levels of PCO2 also resulted in significant increases (71-81%) in total cellular toxicity (fg saxitoxin equivalents/cell) in the Northport Bay strain, while no changes in toxicity were detected in the Bay of Fundy strain. The positive relationship between PCO2 enrichment and elevated growth was reproducible in natural populations from New York waters. Alexandrium densities were significantly and consistently enhanced when natural populations were incubated at 150 Pa PCO2 compared to 39 Pa. During natural Alexandrium blooms in Northport Bay, PCO2 concentrations increased over the course of a bloom to more than 170 Pa and were highest in regions with the greatest Alexandrium abundances, suggesting Alexandrium may further exacerbate acidification and/or be especially adapted to these acidi-fied conditions. The co-occurrence of Alexandrium blooms and elevated PCO2 represents a previously unrecognized, compounding environmental threat to coastal ecosystems. The ability of elevated PCO2 to enhance the growth and toxicity of Alexandrium indicates that acidification promoted by eutrophication or climate change can intensify these, and perhaps other, harmful algal blooms.
    Keywords: Alexandrium fundyense; Alkalinity, total; Alkalinity, total, standard deviation; Aragonite saturation state; Bicarbonate ion; Bottles or small containers/Aquaria (〈20 L); Calcite saturation state; Calculated using CO2SYS; Calculated using seacarb after Nisumaa et al. (2010); Carbon, inorganic, dissolved; Carbon, inorganic, dissolved, standard deviation; Carbonate ion; Carbonate system computation flag; Carbon dioxide; Cell density; Chromista; Coulometric titration; Date; Event label; EXP; Experiment; Fugacity of carbon dioxide (water) at sea surface temperature (wet air); Growth/Morphology; Growth rate; Immunology/Self-protection; Incubation duration; Infrared spectrometric; Laboratory experiment; Laboratory strains; Myzozoa; North Atlantic; Northport_Harbor; OA-ICC; Ocean Acidification International Coordination Centre; Partial pressure of carbon dioxide, standard deviation; Partial pressure of carbon dioxide (water) at sea surface temperature (wet air); Pelagos; pH; pH, standard deviation; Phosphate; Phytoplankton; Potentiometric; Salinity; Silicate; Single species; Species; Strain; Temperature, water; Toxicity, cellular; Treatment
    Type: Dataset
    Format: text/tab-separated-values, 2149 data points
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  • 6
    Publication Date: 2024-03-15
    Keywords: Alkalinity, total; Allele; Animalia; Aragonite saturation state; Bicarbonate ion; Bottles or small containers/Aquaria (〈20 L); Brackish waters; Calcite saturation state; Calculated using CO2SYS; Calculated using seacarb after Nisumaa et al. (2010); Carbon, inorganic, dissolved; Carbonate ion; Carbonate system computation flag; Carbon dioxide; Chordata; Coulometric titration; Date; DATE/TIME; EXP; Experiment; Fugacity of carbon dioxide (water) at sea surface temperature (wet air); Gene expression (incl. proteomics); Group; Identification; Laboratory experiment; LATITUDE; Locus; Long_Island; LONGITUDE; Menidia menidia; Mortality/Survival; Nekton; North Atlantic; OA-ICC; Ocean Acidification International Coordination Centre; Partial pressure of carbon dioxide (water) at sea surface temperature (wet air); Pelagos; pH; Potentiometric; Replicate; Salinity; Sample ID; Single species; Species; Status; Temperate; Temperature, water; Time in days
    Type: Dataset
    Format: text/tab-separated-values, 202769 data points
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  • 7
    Publication Date: 2024-03-15
    Description: While ocean acidification has different effects on herbivores and autotrophs, how acidification may influence herbivory is poorly understood. This study examined how grazing by the gastropod Lacuna vincta (hereafter Lacuna) on the macroalgae Ulva spp. (hereafter Ulva) is influenced by ocean acidification. Herbivory by Lacuna was significantly reduced under elevated partial pressure of carbon dioxide ( pCO2; 1500-2000 µatm) relative to ambient pCO2 (400 µatm). This significant decrease in herbivory was unrelated to the physiological status of Ulva but rather was specifically elicited when Lacuna was exposed to elevated pCO2 in the absence of food for 18 to 24 h prior to grazing Ulva. The negative effects of elevated pCO2 on Lacuna were absent at 400 to 800 µatm pCO2 or when fed but persisted for up to 72 h following a 24 h exposure to elevated pCO2 without food. Depressed respiration rates in Lacuna following exposure to high pCO2 without food indicated these conditions produced metabolic suppression potentially associated with acidosis. Collectively, the lasting (72 h) nature of grazing inhibition of Lacuna following brief exposure (18 h) to moderate pCO2 levels (〉1500 µatm) when food was not available suggests this process could have broad effects on the dynamics of macroalgae in estuaries where Lacuna is a dominant grazer; these effects will be amplified as climate change progresses.
    Keywords: Alkalinity, total; Alkalinity, total, standard deviation; Animalia; Aragonite saturation state; Aragonite saturation state, standard deviation; Behaviour; Benthic animals; Benthos; Bicarbonate ion; Bicarbonate ion, standard deviation; Calcite saturation state; Calcite saturation state, standard deviation; Calculated using CO2SYS; Calculated using seacarb after Nisumaa et al. (2010); Calculated using seacarb after Orr et al. (2018); Carbon, inorganic, dissolved; Carbon, inorganic, dissolved, standard deviation; Carbonate ion; Carbonate ion, standard deviation; Carbonate system computation flag; Carbon dioxide; Carbon dioxide, standard deviation; Coast and continental shelf; Containers and aquaria (20-1000 L or 〈 1 m**2); EXP; Experiment; Fugacity of carbon dioxide (water) at sea surface temperature (wet air); Fugacity of carbon dioxide in seawater, standard deviation; Herbivory rate per grazer; Herbivory rate per grazer, standard deviation; Identification; Laboratory experiment; Lacuna vincta; Mollusca; North Atlantic; OA-ICC; Ocean Acidification International Coordination Centre; Other; Partial pressure of carbon dioxide, standard deviation; Partial pressure of carbon dioxide (water) at sea surface temperature (wet air); pH; pH, standard deviation; Registration number of species; Respiration; Respiration rate, oxygen; Respiration rate, oxygen, standard deviation; Salinity; Salinity, standard deviation; Shinnecock_Bay; Single species; Species; Temperate; Temperature, water; Temperature, water, standard deviation; Treatment; Type; Uniform resource locator/link to reference
    Type: Dataset
    Format: text/tab-separated-values, 1612 data points
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  • 8
    Publication Date: 2024-03-15
    Description: Acidification and deoxygenation are two consequences of climate change that also co-occur in eutrophied coastal zones and can have deleterious effects on marine life. While the effects of hypoxia on marine herbivores have been well-studied, how ocean acidification combined with hypoxia affects herbivory is poorly understood. This study examined how herbivory and survival by the gastropod Lacuna vincta grazing on the macroalgae Ulva rigida was influenced by hypoxia and ocean acidification, alone and in combination, with and without food limitation. Experiments exposed L. vincta to a range of environmentally realistic dissolved oxygen (0.7-8 mg/L) and pH (7.3-8.0 total scale) conditions for 3-72 h, with and without a starvation period and quantified herbivory and survival. While acidified conditions (pH 〈 7.4) reduced herbivory when combined with food limitation, low oxygen conditions (〈 4 mg/L) reduced herbivory and survival regardless of food supply. When L. vincta were starved and grazed in acidified conditions herbivory was additively reduced, whereas starvation and hypoxia synergistically reduced grazing rates. Overall, low oxygen had a more inhibitory effect on herbivory than low pH. Shorter exposure times (9, 6, and 3 h) were required to reduce grazing at lower DO levels (∼2.4, ∼1.6, and ∼0.7 mg/L, respectively). Herbivory ceased entirely following a three-hour exposure to DO of 0.7 mg/L suggesting that episodes of diurnal hypoxia disrupt grazing by these gastropods. The suppression of herbivory in response to acidified and hypoxic conditions could create a positive feedback loop that promotes 'green tides' whereby reduced grazing facilitates the overgrowth of macroalgae that cause nocturnal acidification and hypoxia, further disrupting herbivory and promoting the growth of macroalgae. Such feedback loops could have broad implications for estuarine ecosystems where L. vincta is a dominant macroalgal grazer and will intensify as climate change accelerates.
    Keywords: Alkalinity, total; Alkalinity, total, standard deviation; Animalia; Aragonite saturation state; Aragonite saturation state, standard deviation; Behaviour; Benthic animals; Benthos; Bicarbonate ion; Bicarbonate ion, standard deviation; Bottles or small containers/Aquaria (〈20 L); Calcite saturation state; Calcite saturation state, standard deviation; Calculated using CO2SYS; Calculated using seacarb after Nisumaa et al. (2010); Calculated using seacarb after Orr et al. (2018); Carbon, inorganic, dissolved; Carbon, inorganic, dissolved, standard deviation; Carbonate ion; Carbonate ion, standard deviation; Carbonate system computation flag; Carbon dioxide; Carbon dioxide, standard deviation; Coast and continental shelf; EXP; Experiment; Experiment duration; Figure; Fugacity of carbon dioxide (water) at sea surface temperature (wet air); Fugacity of carbon dioxide in seawater, standard deviation; Herbivory rate per grazer; Herbivory rate per grazer, standard deviation; Laboratory experiment; Lacuna vincta; Mollusca; North Atlantic; OA-ICC; Ocean Acidification International Coordination Centre; Other; Oxygen; Oxygen, dissolved; Oxygen, dissolved, standard deviation; Partial pressure of carbon dioxide, standard deviation; Partial pressure of carbon dioxide (water) at sea surface temperature (wet air); pH; pH, standard deviation; Registration number of species; Salinity; Salinity, standard deviation; Shinnecock_Bay; Single species; Species; Temperate; Temperature, water; Temperature, water, standard deviation; Treatment; Type; Uniform resource locator/link to reference
    Type: Dataset
    Format: text/tab-separated-values, 2108 data points
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  • 9
    Publication Date: 2024-03-15
    Description: The laminarialean kelp, Saccharina latissima, is a common macroalgae along rocky shorelines that is also frequently used in aquaculture. This study examined how ocean acidification may alter the growth of S. latissima as well as grazing on S. latissima by the gastropod, Lacuna vincta. Under elevated nutrients, S. latissima experienced significantly enhanced growth at pCO2 levels 〉1,200 µatm compared to ambient pCO2 (400 µatm). Elevated pCO2 (〉830 µatm) also significantly reduced herbivory of L. vincta grazing on S. latissima relative to ambient pCO2. There was no difference in grazing of S. latissima previously grown under elevated or ambient pCO2, suggesting lowered herbivory was due to harm to the gastropods rather than alteration of the biochemical composition of the kelp. Decreased herbivory was specifically elicited when L. vincta were exposed to elevated pCO2 in the absence of food for 〉18 h prior to grazing, with reduced grazing persisting 72 h. Elevated growth of S. latissima and reduced grazing by L. vincta at 1,200 µatm pCO2 combined to increase net growth rates of S. latissima by more than four-fold relative to ambient pCO2. L. vincta consumed 70% of daily production by S. latissima under ambient pCO2 but only 38% and 9% at 800 µatm and 1,200 µatm, respectively. Collectively, decreased grazing by L. vincta coupled with enhanced growth of S. latissima under elevated pCO2 demonstrates that increased CO2 associated with climate change and/or coastal processes will dually benefit commercially and ecologically important kelps by both promoting growth and reducing grazing pressure.
    Keywords: Alkalinity, total; Alkalinity, total, standard deviation; Animalia; Aragonite saturation state; Aragonite saturation state, standard deviation; Behaviour; Benthic animals; Benthos; Bicarbonate ion; Bicarbonate ion, standard deviation; Bottles or small containers/Aquaria (〈20 L); Calcite saturation state; Calcite saturation state, standard deviation; Calculated using CO2SYS; Calculated using seacarb after Nisumaa et al. (2010); Calculated using seacarb after Orr et al. (2018); Carbon, inorganic, dissolved; Carbon, inorganic, dissolved, standard deviation; Carbon, per tissue dry mass; Carbon, standard deviation; Carbon/Nitrogen ratio; Carbon/Nitrogen ratio, standard deviation; Carbonate ion; Carbonate ion, standard deviation; Carbonate system computation flag; Carbon dioxide; Carbon dioxide, standard deviation; Chromista; Coast and continental shelf; Duration; EXP; Experiment; Figure; Fugacity of carbon dioxide (water) at sea surface temperature (wet air); Fugacity of carbon dioxide in seawater, standard deviation; Growth/Morphology; Growth rate; Growth rate, standard deviation; Herbivory rate per grazer; Herbivory rate per grazer, standard deviation; Laboratory experiment; Lacuna vincta; Macroalgae; Macro-nutrients; Mollusca; Nitrogen, per tissue dry mass; Nitrogen, standard deviation; North Atlantic; OA-ICC; Ocean Acidification International Coordination Centre; Ochrophyta; Other; Partial pressure of carbon dioxide, standard deviation; Partial pressure of carbon dioxide (water) at sea surface temperature (wet air); pH; pH, standard deviation; Potentiometric; Registration number of species; Saccharina latissima; Salinity; Salinity, standard deviation; Shinnecock_Bay; Single species; Species; Species interaction; Temperate; Temperature, water; Temperature, water, standard deviation; Treatment; Type; Uniform resource locator/link to reference; δ13C; δ13C, standard deviation
    Type: Dataset
    Format: text/tab-separated-values, 1422 data points
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  • 10
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    PANGAEA
    In:  Supplement to: Talmage, Stephanie C; Gobler, Christopher J (2010): Effects of past, present, and future ocean carbon dioxide concentrations on the growth and survival of larval shellfish. Proceedings of the National Academy of Sciences of the United States of America, 107(40), 17246-17251, https://doi.org/10.1073/pnas.0913804107
    Publication Date: 2024-03-15
    Description: The combustion of fossil fuels has enriched levels of CO2 in the world's oceans and decreased ocean pH. Although the continuation of these processes may alter the growth, survival, and diversity of marine organisms that synthesize CaCO3shells, the effects of ocean acidification since the dawn of the industrial revolution are not clear. Here we present experiments that examined the effects of the ocean's past, present, and future (21st and 22nd centuries) CO2concentrations on the growth, survival, and condition of larvae of two species of commercially and ecologically valuable bivalve shellfish (Mercenaria mercenariaand Argopecten irradians). Larvae grown under near preindustrial CO2concentrations (250 ppm) displayed significantly faster growth and metamorphosis as well as higher survival and lipid accumulation rates compared with individuals reared under modern day CO2 levels. Bivalves grown under near preindustrial CO2 levels displayed thicker, more robust shells than individuals grown at present CO2 concentrations, whereas bivalves exposed to CO2 levels expected later this century had shells that were malformed and eroded. These results suggest that the ocean acidification that has occurred during the past two centuries may be inhibiting the development and survival of larval shellfish and contributing to global declines of some bivalve populations.
    Keywords: Alkalinity, total; Alkalinity, total, standard deviation; Animalia; Aragonite saturation state; Aragonite saturation state, standard deviation; Argopecten irradians; Argopecten irradians, diameter; Argopecten irradians, diameter, standard deviation; Argopecten irradians, lipid index; Argopecten irradians, lipid index, standard deviation; Argopecten irradians, metamorphosed; Argopecten irradians, pediveliger; Argopecten irradians, thickness; Argopecten irradians, thickness, standard deviation; Argopecten irradians, veliger; Bicarbonate ion; Bottles or small containers/Aquaria (〈20 L); Calcite saturation state; Calcite saturation state, standard deviation; Calculated using CO2SYS; Calculated using seacarb after Nisumaa et al. (2010); Carbon, inorganic, dissolved; Carbon, inorganic, dissolved, standard deviation; Carbonate ion; Carbonate ion, standard deviation; Carbonate system computation flag; Carbon dioxide; Carbon dioxide, partial pressure, standard deviation; Coast and continental shelf; Development; EGM-4 Environmental Gas Analyzer (PP Systems); EPOCA; EUR-OCEANS; European network of excellence for Ocean Ecosystems Analysis; European Project on Ocean Acidification; Experimental treatment; Experiment day; Fugacity of carbon dioxide (water) at sea surface temperature (wet air); Growth/Morphology; Laboratory experiment; Mercenaria mercenaria; Mercenaria mercenaria, lipid index; Mercenaria mercenaria, lipid index, standard deviation; Mercenaria mercenaria, metamorphosed; Mercenaria mercenaria, pediveliger; Mercenaria mercenaria, shell size; Mercenaria mercenaria, shell size, standard deviation; Mercenaria mercenaria, thickness; Mercenaria mercenaria, thickness, standard deviation; Mercenaria mercenaria, veliger; Mollusca; Mortality/Survival; North Atlantic; OA-ICC; Ocean Acidification International Coordination Centre; Partial pressure of carbon dioxide (water) at sea surface temperature (wet air); Pelagos; pH; pH, standard deviation; Salinity; Salinity, standard deviation; see reference(s); Single species; Species; Temperature, standard deviation; Temperature, water; Tropical; Zooplankton
    Type: Dataset
    Format: text/tab-separated-values, 2628 data points
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