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  • 1
    ISSN: 1573-6881
    Keywords: cytochrome bc 1 complex ; electron transfer reaction: mitochondrial-processing peptidase ; superoxide-generation activity ; electron transfer inhibitors
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology , Physics
    Notes: Abstract The mitochondrial cytochrome bc 1 complex is a multifunctional membrane protein complex. Itcatalyzes electron transfer, proton translocation, peptide processing, and superoxide generation.Crystal structure data at 2.9 Å resolution not only establishes the location of the redox centersand inhibitor binding sites, but also suggests a movement of the head domain of the iron–sulfurprotein (ISP) during bc 1 catalysis and inhibition of peptide-processing activity during complexmaturation. The functional importance of the movement of extramembrane (head) domain ofISP in the bc 1 complex is confirmed by analysis of the Rhodobacter sphaeroides bc 1 complexmutants with increased rigidity in the ISP neck and by the determination of rate constants foracid/base-induced intramolecular electron transfer between [2Fe–2S] and heme c 1 in nativeand inhibitor-loaded beef complexes. The peptide-processing activity is activated in bovineheart mitochondrial bc 1 complex by nonionic detergent at concentrations that inactivate electrontransfer activity. This peptide-processing activity is shown to be associated with subunits Iand II by cloning, overexpression and in vitro reconstitution. The superoxide-generation siteof the cytochrome bc 1 complex is located at reduced b L and Q•−. The reaction is membranepotential-, and cytochrome c-dependent.
    Type of Medium: Electronic Resource
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  • 2
    Publication Date: 2022-04-05
    Description: Abstract The novel 10Be (meteoric)/9Be system, where 10Be is delivered by precipitation and stable 9Be is released by weathering, provides denudation rates over weathering‐erosion timescales. The new tool is applicable to quartz‐poor lithologies, for example, mafic rock and claystone, which are not readily accessible by the commonly used in situ‐produced 10Be in quartz. We provide a first application of this proxy to a tectonically active mountainous river, the Zhuoshui River in Taiwan. Taiwan Rivers supply a disproportionately high suspended and dissolved flux to the oceans and are often underlain by fine‐grained shale/slate. 10Be (meteoric)/9Be‐derived denudation rates (Dmet) from the Zhuoshui Catchment are highest in the slate‐dominated headwaters (4–8 mm/year), and much lower (1–2 mm/year) along the midlower reaches with mixed lithologies. At the basin‐wide scale, we find a poor correlation between Dmet and basin‐averaged channel steepness despite a small climatic gradient. Because large lithological heterogeneities exist in this basin, we invoke a lithological effect to explain this poor correlation. Relying on a revised stream power incision model that incorporates rock erodibility, the resulting lithology‐ and runoff‐adjusted ksn (kLrsn) can be reconciled with denudation rates with the highest erodibility predicted to prevail in the Miocene slate of low metamorphic grade and high fracture density. This model suggests that the lithological heterogeneity can alter the coupling between surface denudation and channel morphology. On a broader perspective, the successful application of the 10Be (meteoric)/9Be proxy shows its applicability as a tracer for erosion and sediment transport processes in fast‐eroding mountain belts underlain by slate lithologies.
    Description: Key Points 10Be (meteoric)/9Be ratios quantify fast denudation of slate regions in Taiwan Topographic metrics and denudation rates show different spatial patterns Lithologic variability alters coupling between denudation rates and ksn, based on a revised stream power model including rock erodibility
    Description: Freie Universität Berlin‐China Scholarship Council PhD Program
    Description: National Natural Science Foundation of China http://dx.doi.org/10.13039/501100001809
    Keywords: ddc:551.3
    Language: English
    Type: doc-type:article
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  • 3
    Publication Date: 2013-07-05
    Description: The prokaryotic diversity in two brackish lakes (Sayram Lake and Chaiwopu Lake) was investigated by constructing bacterial and archaeal clone libraries of 16S rRNA genes. Bacterial clones from Sayram Lake were classified into six phyla (Proteobacteria, Verrucomicrobia, Bacteroidetes, Planctomycetes, Acidobacteria, Actinobacteria). Of these, Proteobacteria and Verrucomicrobia were the most dominant, representing 50.4 and 16.8% of the clone library, respectively. Sequences related to Proteobacteria (58.1%), Cyanobacteria (17.2%), Bacteroidetes (15%), Verrucomicrobia (4.3%), Actinobacteria (3.2%) constituted over 97% of the bacterial clone library from Chaiwopu Lake. In addition, 58.8% (Sayram Lake) and 48% (Chaiwopu Lake) of bacterial clones showed high sequence identity to pure cultures. The composition of Archaea was obviously different between the two lakes. Only the Crenarchaeota phylum was found in the Sayram Lake, whereas Archaeal sequences from Chaiwopu Lake were classified into three phyla: Crenarchaeota (5.8%), Thaumarchaeota (81.2%), and Euryarchaeota (13%). Among the archaeal sequences, 94.2% were highly related to cultivable species of the genus Nitrosopumilus , Methanoculleus , and Methanobacterium . These results showed a high diversity of potential cultivable heterotrophic bacteria in Sayram Lake and Chaiwopu Lake. Chaiwopu Lake was a source of potentially novel, cultivable archaea.
    Print ISSN: 0233-111X
    Electronic ISSN: 1521-4028
    Topics: Biology
    Published by Wiley-Blackwell
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  • 4
    Publication Date: 2024-02-07
    Description: The ratio of atmosphere-derived 10 Be to continent-derived 9 Be in marine sediments has been used to probe the long-term relationship between continental denudation and climate. However, its application is complicated by uncertainty in 9 Be transfer through the land-ocean interface. The riverine dissolved load alone is insufficient to close the marine 9 Be budget, largely due to substantial removal of riverine 9 Be to continental margin sediments. We focus on the ultimate fate of this latter Be. We present sediment pore-water Be profiles from diverse continental margin environments to quantify the diagenetic Be release to the ocean. Our results suggest that pore-water Be cycling is mainly controlled by particulate supply and Mn-Fe cycling, leading to higher benthic fluxes on shelves. Benthic fluxes may help close the 9 Be budget and are at least comparable to, or higher (~2-fold) than, the riverine dissolved input. These observations demand a revised model framework, which considers the potentially dominant benthic source, to robustly interpret marine Be isotopic records.
    Type: Article , PeerReviewed
    Format: text
    Format: text
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  • 5
    Publication Date: 2013-09-26
    Description: Background: Tumor microenviroment is characteristic of inflammation, ischemia and starvation of nutrient. TNF-alpha, which is an extraordinarily pleiotropic cytokine, could be an endogenous tumor promoter in some tumor types. The basic objective of this study was to investigate the effects of TNF-alpha on the cell viability and apoptosis of hepatocellular carcinoma cells under serum starvation, and to identify the molecular mechanisms involved. Methods: For this purpose, five different concentrations of TNF-alpha and two different serum settings (serum-cultured and serum-deprived) were used to investigate the effects of TNF-alpha on the cell viability and apoptosis of Hep3B and SMMC-7721 cells. Results: TNF-alpha (10 ng/ml) attenuated serum starvation-induced apoptosis of hepatocellular carcinoma cells, and autophagy conferred this process. BAY11-7082, a specific inhibitor of NF-kappaB, reversed the suppression of serum starvation-induced apoptosis by TNF-alpha. Moreover, TNF-alpha-induced NF-kappaB transactivation was suppressed by autophagy inhibitor 3-MA. In addition, TNF-alpha up-regulated Ferritin heavy chain (FHC) transiently by NF-kappaB activation and FHC levels were correlated with the TNF-alpha-induced protection against serum starvation-mediated apoptosis of hepatocellular carcinoma cells. Furthermore, FHC-mediated inhibition of apoptosis depended on suppressing ROS accumulation. Conclusions: Our findings suggested that autophagy conferred the TNF-alpha protection against serum starvation-mediated apoptosis of hepatocellular carcinoma cells, the mechanism involved with the activation of the TNF-alpha/ NF-kappaB /FHC signaling pathway.
    Electronic ISSN: 1471-2407
    Topics: Medicine
    Published by BioMed Central
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  • 6
    Publication Date: 2011-12-01
    Print ISSN: 0037-1106
    Electronic ISSN: 1943-3573
    Topics: Geosciences , Physics
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  • 7
    Publication Date: 2018-05-09
    Description: Remote Sensing, Vol. 10, Pages 722: Speckle Suppression by Weighted Euclidean Distance Anisotropic Diffusion Remote Sensing doi: 10.3390/rs10050722 Authors: Fengcheng Guo Guo Zhang Qingjun Zhang Ruishan Zhao Mingjun Deng Kai Xu To better reduce image speckle noise while also maintaining edge information in synthetic aperture radar (SAR) images, we propose a novel anisotropic diffusion algorithm using weighted Euclidean distance (WEDAD). Presented here is a modified speckle reducing anisotropic diffusion (SRAD) method, which constructs a new edge detection operator using weighted Euclidean distances. The new edge detection operator can adaptively distinguish between homogenous and heterogeneous image regions, effectively generate anisotropic diffusion coefficients for each image pixel, and filter each pixel at different scales. Additionally, the effects of two different weighting methods (Gaussian weighting and non-linear weighting) of de-noising were analyzed. The effect of different adjustment coefficient settings on speckle suppression was also explored. A series of experiments were conducted using an added noise image, GF-3 SAR image, and YG-29 SAR image. The experimental results demonstrate that the proposed method can not only significantly suppress speckle, thus improving the visual effects, but also better preserve the edge information of images.
    Electronic ISSN: 2072-4292
    Topics: Architecture, Civil Engineering, Surveying , Geography
    Published by MDPI Publishing
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  • 8
    Publication Date: 2017-09-02
    Description: Sensors, Vol. 17, Pages 2005: Multi-Mode GF-3 Satellite Image Geometric Accuracy Verification Using the RPC Model Sensors doi: 10.3390/s17092005 Authors: Taoyang Wang Guo Zhang Lei Yu Ruishan Zhao Mingjun Deng Kai Xu The GaoFen-3 (GF-3) satellite is the first C-band multi-polarization synthetic aperture radar (SAR) imaging satellite with a resolution up to 1 m in China. It is also the only SAR satellite of the High-Resolution Earth Observation System designed for civilian use. There are 12 different imaging models to meet the needs of different industry users. However, to use SAR satellite images for related applications, they must possess high geometric accuracy. In order to verify the geometric accuracy achieved by the different modes of GF-3 images, we analyze the SAR geometric error source and perform geometric correction tests based on the RPC model with and without ground control points (GCPs) for five imaging modes. These include the spotlight (SL), ultra-fine strip (UFS), Fine Strip I (FSI), Full polarized Strip I (QPSI), and standard strip (SS) modes. Experimental results show that the check point residuals are large and consistent without GCPs, but the root mean square error of the independent checkpoints for the case of four corner control points is better than 1.5 pixels, achieving a similar level of geometric positioning accuracy to that of international satellites. We conclude that the GF-3 satellite can be used for high-accuracy geometric processing and related industry applications.
    Electronic ISSN: 1424-8220
    Topics: Chemistry and Pharmacology , Electrical Engineering, Measurement and Control Technology
    Published by MDPI Publishing
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  • 9
    Publication Date: 2017-08-30
    Description: Sensors, Vol. 17, Pages 1977: Geometric Calibration and Accuracy Verification of the GF-3 Satellite Sensors doi: 10.3390/s17091977 Authors: Ruishan Zhao Guo Zhang Mingjun Deng Kai Xu Fengcheng Guo The GF-3 satellite is the first multi-polarization synthetic aperture radar (SAR) imaging satellite in China, which operates in the C band with a resolution of 1 m. Although the SAR satellite system was geometrically calibrated during the in-orbit commissioning phase, there are still some system errors that affect its geometric positioning accuracy. In this study, these errors are classified into three categories: fixed system error, time-varying system error, and random error. Using a multimode hybrid geometric calibration of spaceborne SAR, and considering the atmospheric propagation delay, all system errors can be effectively corrected through high-precision ground control points and global atmospheric reference data. The geometric calibration experiments and accuracy evaluation for the GF-3 satellite are performed using ground control data from several regions. The experimental results show that the residual system errors of the GF-3 SAR satellite have been effectively eliminated, and the geometric positioning accuracy can be better than 3 m.
    Electronic ISSN: 1424-8220
    Topics: Chemistry and Pharmacology , Electrical Engineering, Measurement and Control Technology
    Published by MDPI Publishing
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  • 10
    Publication Date: 2014-06-07
    Description: Nature Reviews Neurology 10, 337 (2014). doi:10.1038/nrneurol.2014.78 Authors: Hao Deng, Kai Gao & Joseph Jankovic The neurodegenerative diseases are a diverse group of disorders characterized by progressive loss of specific groups of neurons. These diseases affect different populations, and have a variable age of onset, clinical symptoms, and pathological findings. Variants in the FUS gene, which encodes an RNA-binding
    Topics: Medicine
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