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  • 1
    Electronic Resource
    Electronic Resource
    s.l. : American Chemical Society
    Biochemistry 30 (1991), S. 10269-10273 
    ISSN: 1520-4995
    Source: ACS Legacy Archives
    Topics: Biology , Chemistry and Pharmacology
    Type of Medium: Electronic Resource
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  • 2
    Electronic Resource
    Electronic Resource
    Springer
    Molecular and cellular biochemistry 102 (1991), S. 35-47 
    ISSN: 1573-4919
    Keywords: lactate ; arachidonic acid ; phospholipase A2 ; lipoxygenase ; 15-HETE ; beta-adrenergic receptor
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology , Medicine
    Notes: Abstract Incubation of rocker-cultured neonatal rat heart cells with 3 mM L(+)-lactate led to a sharp increase in the sensitivity of cardiomyocytes to the beta-adrenergic agonist isoprenaline, as measured by their chronotropic response. This effect was accompanied by a reduction in the arachidonic acid content of the total phospholipids. The phospholipase A2-activator melittin as well as free arachidonic acid induced this supersensitivity to the same degree. On the other hand, the L(+)-lactate-evoked supersensitivity could be blocked by the phospholipase A2 inhibitors mepacrine and n-bromophenacyl-bromide, suggesting an involvement of phospholipase A2 in the process of beta-adrenergic sensitization. The sensitizing action of arachidonic acid was blocked by the lipoxygenase inhibitors esculetin and nordihydroguaiaretic acid, but not by the cyclooxygenase inhibitor indomethacin. Supersensitivity was likewise evoked by 15-S-hydroxyeicosatetraenoic acid (15-S-HETE), but not by 5-S-HPETE or 5-S-HETE. These findings suggest that the phospholipase A2-15-lipoxygenase pathway plays a role in the induction of beta-adrenergic supersensitivity in the cultured cardiomyocytes and point to a new physiological role of the lipoxygenase product 15-S-HETE.
    Type of Medium: Electronic Resource
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  • 3
    ISSN: 1573-4919
    Keywords: growth inhibitor ; modulation of beta-adrenergic response ; rat neonatal heart muscle cells ; lipid binding ; fatty acid-binding protein
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology , Medicine
    Notes: Summary ‘Mammary-derived growth inhibitor (MDGI)’ is a 14.5 kDa polypeptide with growth-inhibitory activity for various mammary epithelial cells in vitro which is highly homologous to cardiac fatty acid-binding protein (H-FABP). Here we describe a new biological activity of MDGI: Inhibition of L(+)-lactate-, arachidonic acid- and 15-S-hydroxyeicosatetraenoic acid-induced supersensitivity of neonatal rat heart cells for beta-adrenergic stimulation, concerning particularly a small population of beta2 receptors. Synthetic peptides corresponding to the MDGI-sequence, residue 121–131 mimic the effect of MDGI. Measurements of lipid-binding to MDGI and synthetic peptides excluded the binding of arachidonic acid, 15-S-hydroxyeicosatetraenoic acid or beta-adrenergic agonists to MDGI or the peptides as the mechanism for this effect. Also, no direct interference of MDGI and the synthetic peptides with the binding of the beta-adrenergic agent CGP 12177 to its receptor on A431 cells could be detected. We suggest that MDGI and the peptides act by interference with the function of the beta2-adrenergic receptor and that this mechanism might also be relevant for the growth-inhibitory activity of MDGI. Furthermore, the data point to a possible function of H-FABP for the modulation of beta-adrenergic sensitivity of cardiac myocytes.
    Type of Medium: Electronic Resource
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  • 4
    Publication Date: 2016-08-08
    Repository Name: EPIC Alfred Wegener Institut
    Type: Conference , notRev
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  • 5
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    Copernicus
    In:  EPIC3European Geosciences Union EGU General Assembly, Vienna, Austria, 2018-04-08-2018-04-13Copernicus
    Publication Date: 2018-06-18
    Description: We use a comprehensive suite of partially laminated high-resolution sediment cores from the Bering Sea, covering a depth transect from 1100 m to 2700 m to study deglacial surface ocean warming patterns, associated changes in biological productivity, oxygen minimum zone dynamics and continent-ocean links through Yukon river runoff. We apply a combination of planktic and benthic isotopes, x-ray fluorescence (XRF)-derived ele- mental ratios and a multi-proxy assessment of changes in upper ocean temperatures. Severe oxygen depletions occurred during the Bølling/Allerød (B/A) and early Holocene, which is in accordance with other locations in the North Pacific, especially the Alaska margin. Detailed analysis of the timing of lamination occurrence between the different sediment cores revealed that the onset of severe anoxia at the beginning of the B/A and early Holocene is a near-synchronous event, while the disappearance of laminations is a diachronic process. The deglacial Oxygen Minimum Zone(OMZ) strengthening is mainly driven by increased export production, visible in XRF-derived elemental ratios, and corresponding high accumulation rates of biogenic components. The export production in turn is a response to rising sea surface temperatures, decreased sea ice cover and increased thermal stratification, while a major nutrient source was the eastern continental shelf, which was flooded during the deglacial global sea level rise. It is discussed controversially whether oxygenation variations in the deglacial subarctic Pacific were coupled to changes in mid-depth water chemistry, or rather a response to physical processes like deep-intermediate ocean or mixed layer warming, or stratification changes. However, knowledge of the driving forcing mechanism for OMZ strengthening is of particular importance, as these are tightly coupled to the regional marine carbon budget, e.g. via the strength and efficiency of the biological pump. Here, our laminated sediments provided the opportunity to study ocean dynamics in exceptional detail, possible on decadal to annual timescales. Due to the correlation patterns of our records to the NGRIP oxygen isotope record through layer counts we presume that (i) the presence of laminations is tightly coupled to submillennial, short-term warm phases, especially during the Bølling-Allerød (B/A), (ii) that the laminations represent annual layered sediments (varves). The latter point in conjunction with our geochemical proxies strongly supports an atmospheric teleconnection between SE Asia, the North Atlantic and the North Pacific, with observed changes in mid-depth ocean dynamics occurring on fast, nearly decadal timescales. Thus, the Bering Sea OMZ is a highly sensitive system reacting almost instantaneously to small temperature changes and therefore has the potential to influence the global carbon budget on short timescales, in particular during episodes of rapidly warming climate.
    Repository Name: EPIC Alfred Wegener Institut
    Type: Conference , notRev
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  • 6
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    http://www.suub.uni-bremen.de
    In:  EPIC3http://www.suub.uni-bremen.de, 120 p.
    Publication Date: 2016-12-21
    Description: In the past decades several paleoceanographic studies found evidence for millennial scale climate variability during the late Pleistocene. While these climate fluctuations are well studied in the North Atlantic, the paleoceanographic history in the North Pacific is less well known. The last glacial termination is of special interest, as it represents the most recent transition from glacial to deglacoal conditions. In the North Pacific these changes led to a strengthening of the mid-depth oxygen minimum zone (OMZ) to anoxic bottom water conditions and the deposition of laminated sediments. The main objective of this thesis was to gain new insights into the deglacial mid-depth OMZ dynamics in the Bering Sea, using a suite of mid-depth, partly laminated sediment cores that were collected during R/V Sonne cruise SO202-INOPEX. It will be shown that during the Bølling–Allerød (BA) and early Holocene the Bering Sea OMZ strengthened to anoxic values of 〈0.1 ml/l, which led to the formation of laminations, and expanded vertically to water depths of 〉2100 m. Based on a correlation of 14C-dated, laminated sediment cores it is revealed that throughout the Bering Sea and the Gulf of Alaska the onset of deglacial anoxia at 14.6 ka and 11.7 ka was a synchronous event, while the disappearance of laminations was a diachronous process. A decadal-scale correlation of two 14C dated, layer counted sediment cores with seasonal resolution from the northeastern Bering Sea slope to the NGRIP δ18O record revealed, that (1) the formation of laminations was tightly coupled to warm phases of the B/A and the early Holocene, which implies an atmospheric teleconnection between the North Atlantic and Bering Sea, and (2) the presence of annually laminated sediments (varves). The established age model was partly independent from radiocarbon ages and allowed the calculation of surface reservoir ages, which are 770 yr, 910yr and 875 yr for the Holocene, Younger Dryas and B/A respectively. The anoxia were driven on millennial scales by basin-wide remineralization of organic matter in intermediate waters, in combination with decadal-scale export productivity increases during times of warm temperatures. Based on a stacked sea surface temperature (SST) record, SSTs of about 6 °C persisted during times of lamina formations, while SSTs showed values of about 5 °C during times were laminations were not widespread. Other factors that enhanced the export productivity during the B/A and Holocene were increased stratification, a change towards seasonal sea ice, and an input of nutrients and organic carbon from the Alaska hinterlands, which started at 16.6 ka in the Bering Sea. Spectral analyses of Xray-fluorescense-based Cl values of single laminae, which were used as a indicator for biosiliceous productivity, revealed a sharp spectral peak of 18.8 yr and a broader peak of 30 – 60 yr in the B/A and early Holocene. The first peak was related to the 18.6 yr nodal tidal cycle and the latter peak to the Pacific Decadal Oscillation. They influenced primary productivity through nutrient delivery to the surface via tidal mixing, SST variations and stratification. Both processes act in the modern subarctic Pacific, which suggests that productivity variations in the subarctic Pacific are consistently influenced by a combination of external and internal forcing mechanisms since the last deglacial.
    Repository Name: EPIC Alfred Wegener Institut
    Type: Thesis , notRev
    Format: application/pdf
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  • 7
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    In:  EPIC335th International Geological Conference, Cape Town, South Africa, 2016-08-29-2016-09-02
    Publication Date: 2018-08-10
    Repository Name: EPIC Alfred Wegener Institut
    Type: Conference , notRev
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  • 8
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    In:  EPIC3"Scales and Scaling in the Climate System" Workshop (GEOTOP - McGill - CLISAP - PAGES), Jouvence (Montreal), Canada, 2015-10-05-2015-10-07
    Publication Date: 2015-11-26
    Repository Name: EPIC Alfred Wegener Institut
    Type: Conference , notRev
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  • 9
    Publication Date: 2015-11-27
    Repository Name: EPIC Alfred Wegener Institut
    Type: Conference , notRev
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  • 10
    Publication Date: 2019-03-07
    Repository Name: EPIC Alfred Wegener Institut
    Type: Conference , notRev
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