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  • BIOPRESS LIMITED  (2)
  • MDPI  (2)
  • Nature Publishing Grop  (1)
  • 1
    Publication Date: 2021-02-02
    Description: The Southern Ocean is one of the most productive ecosystems in the world. It is an area heavily dependent on marine primary production and serving as a feeding ground for numerous seabirds and marine mammals. Therefore, the phytoplankton composition and presence of toxic species are of crucial importance. Fifteen monoclonal strains of Pseudo-nitzschia subcurvata, a diatom species endemic to the Southern Ocean, were established, which were characterized by morphological and molecular data and then analysed for toxin content. The neurotoxins domoic acid and isodomoic acid C were present in three of the strains, which is a finding that represents the first evidence of these toxins in strains from Antarctic waters. Toxic phytoplankton in Antarctic waters are still largely unexplored, and their effects on the ecosystem are not well understood. Considering P. subcurvata’s prevalence throughout the Southern Ocean, these results highlight the need for further investigations of the harmful properties on the Antarctic phytoplankton community as well as the presence of the toxins in the Antarctic food web, especially in the light of a changing climate.
    Repository Name: EPIC Alfred Wegener Institut
    Type: Article , isiRev
    Format: application/pdf
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  • 2
    Publication Date: 2018-09-11
    Description: The recently established diatom genus Shionodiscus is characterized as possessing a labiate process on the valve face and strutted processes with long internal and reduced, or no, external extensions. Many Thalassiosira species with these features were transferred to this new genus, as well as some with short internal, strutted process extensions. Examination of samples from the Argentine continental shelf and slope waters, and from the Beagle Channel, revealed the presence of a small centric diatom that formed an extensive bloom in slope waters during spring 2005 and 2006. Analysis by light and electron microscopy revealed high similarity to specimens previously referred as Thalassiosira bioculata var. raripora. This name was never effectively published; nevertheless, the name has been adopted and observations referring to it have been reported on several occasions. Here we trace the usage of the (nomenclaturally invalid) name Thalassiosira bioculata var. raripora in the literature, and formally describe the species as Shionodiscus gaarderae, based on our observations and comparison with previous reports. A morphological comparison of this and similar taxa is also provided. In addition, we offer an emended description of S. bioculatus var. bioculatus from Ostenfeld material sampled from the "Färöer Island Naströ Fjord Plankton (16.9.1902)”, held in the Hustedt collection. Finally, we propose the transfer of Thalassiosira rosulata to Shionodiscus.
    Repository Name: EPIC Alfred Wegener Institut
    Type: Article , isiRev
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  • 3
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    BIOPRESS LIMITED
    In:  EPIC3Diatom Research, BIOPRESS LIMITED, 30(1), ISSN: 0269-249X
    Publication Date: 2015-10-27
    Description: Vanhoeffenus antarcticus was described by Heinrich Heiden from a net sample taken from 385 m depth by the Gauss expedition (First German Antarctic Expedition) while frozen in pack ice near the Antarctic continent in the austral summer of 1902–3. Valves of this peculiar diatom have only ever been observed in preparations from this locality and only using light microscopy, but an electron microscopy study of this taxon has become possible now that some surviving material from the Gauss expedition has been recovered. Our scanning electron microscopy observations show the presence of a radially arranged system of marginal chambers, connected to hollow radial rays and continuing through unusually long processes to the inside of the cell. Another unique characteristic of this taxon is a marginal ring of pillars bearing hooks rising from the valve mantle–valve face boundary, previously interpreted (based on light microscopy) as a ring of T-shaped spines. However, despite the improved understanding of its valve structure, the systematic position of this taxon and its biology remain enigmatic.
    Repository Name: EPIC Alfred Wegener Institut
    Type: Article , isiRev
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  • 4
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    MDPI
    In:  EPIC3Applied Sciences, MDPI, 7(4), pp. 330-330, ISSN: 2076-3417
    Publication Date: 2017-05-22
    Description: Light microscopy analysis of diatom frustules is widely used in basic and applied research, notably taxonomy, morphometrics, water quality monitoring and paleo-environmental studies. Although there is a need for automation in these applications, various developments in image processing and analysis methodology supporting these tasks have not become widespread in diatom-based analyses. We have addressed this issue by combining our automated diatom image analysis software SHERPA with a commercial slide-scanning microscope. The resulting workflow enables mass-analyses of a broad range of morphometric features from individual frustules mounted on permanent slides. Extensive automation and internal quality control of the results helps to minimize user intervention, but care was taken to allow the user to stay in control of the most critical steps (exact segmentation of valve outlines and selection of objects of interest) using interactive functions for reviewing and revising results. In this contribution, we describe our workflow and give an overview of factors critical for success, ranging from preparation and mounting through slide scanning and autofocus finding to final morphometric data extraction. To demonstrate the usability of our methods we finally provide an example application by analysing Fragilariopsis kerguelensis valves originating from a sediment core, which substantially extends the size range reported in the literature.
    Repository Name: EPIC Alfred Wegener Institut
    Type: Article , isiRev
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  • 5
    Publication Date: 2022-08-15
    Description: Eukaryotic phytoplankton are responsible for at least 20% of annual global carbonfixation.Their diversity and activity are shaped by interactions with prokaryotes as part of complexmicrobiomes. Although differences in their local species diversity have been estimated, westill have a limited understanding of environmental conditions responsible for compositionaldifferences between local species communities on a large scale from pole to pole. Here, weshow, based on pole-to-pole phytoplankton metatranscriptomes and microbial rDNAsequencing, that environmental differences between polar and non-polar upper oceans moststrongly impact the large-scale spatial pattern of biodiversity and gene activity in algalmicrobiomes. The geographic differentiation of co-occurring microbes in algal microbiomescan be well explained by the latitudinal temperature gradient and associated break points intheir beta diversity, with an average breakpoint at 14 °C ± 4.3, separating cold and warmupper oceans. As global warming impacts upper ocean temperatures, we project that breakpoints of beta diversity move markedly pole-wards. Hence, abrupt regime shifts in algalmicrobiomes could be caused by anthropogenic climate change.
    Repository Name: EPIC Alfred Wegener Institut
    Type: Article , NonPeerReviewed
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