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  • 2020-2024  (25)
  • 2000-2004  (7)
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  • 11
    Publication Date: 2024-03-15
    Description: Coral reefs are constructed by calcifiers that precipitate calcium carbonate to build their shells or skeletons through the process of calcification. Accurately assessing coral calcification rates is crucial to determine the health of these ecosystems and their response to major environmental changes such as ocean warming and acidification. Several approaches have been used to assess rates of coral calcification but there is a real need to compare these approaches in order to ascertain that high quality and intercomparable results can be produced. Here, we assessed four methods (total alkalinity anomaly, calcium anomaly, 45Ca incorporation and 13C incorporation) to determine coral calcification of the reef-building coral Stylophora pistillata. Given the importance of environmental conditions on this process, the study was performed under two pH (ambient and low level) and two light (light and dark) conditions. Under all conditions, calcification rates estimated using the alkalinity and calcium anomaly techniques as well as 45Ca incorporation were highly correlated. Such a strong correlation between the alkalinity anomaly and 45Ca incorporation techniques has not been observed in previous studies and most probably results from improvements described in the present paper. The only method which provided calcification rates significantly different from the other three techniques was 13C incorporation. Calcification rates based on this method were consistently higher than those measured using the other techniques. Although reasons for these discrepancies remain unclear, the use of this technique for assessing calcification rates in corals is not recommended without further investigations.
    Keywords: Alkalinity, total; Alkalinity, total, standard deviation; Animalia; Aragonite saturation state; Aragonite saturation state, standard deviation; Benthic animals; Bicarbonate ion; Bicarbonate ion, standard deviation; Bottles or small containers/Aquaria (〈20 L); Calcification/Dissolution; Calcification rate, standard error; Calcification rate of calcium carbonate; Calcite saturation state; Calcite saturation state, standard deviation; Calcium; Calcium-45 activity; Calcium-45 activity, standard deviation; Calcium ion, standard deviation; Calculated using seacarb after Nisumaa et al. (2010); Calculated using seacarb after Orr et al. (2018); Carbon, inorganic, dissolved; Carbon, inorganic, dissolved, standard deviation; Carbonate ion; Carbonate ion, standard deviation; Carbonate system computation flag; Carbon dioxide; Carbon dioxide, standard deviation; Cnidaria; Date/time end; Date/time start; Dry mass; Experiment; Fugacity of carbon dioxide (water) at sea surface temperature (wet air); Fugacity of carbon dioxide in seawater, standard deviation; Identification; Incubation duration; Laboratory experiment; Laboratory strains; Light; Mass; Not applicable; OA-ICC; Ocean Acidification International Coordination Centre; Partial pressure of carbon dioxide, standard deviation; Partial pressure of carbon dioxide (water) at sea surface temperature (wet air); pH; Potentiometric; Potentiometric titration; Registration number of species; Salinity; Single species; Size; Species; Stylophora pistillata; Temperature, water; Temperature, water, standard deviation; Treatment; Type; Uniform resource locator/link to reference; δ13C; δ13C, standard deviation
    Type: Dataset
    Format: text/tab-separated-values, 2610 data points
    Location Call Number Limitation Availability
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  • 12
    Publication Date: 2024-03-15
    Description: Ocean acidification is perceived to be a major threat for many calcifying organisms, including scleractinian corals. Here we investigate (1) whether past exposure to low pH environments associated with CO2 vents could increase corals tolerance to low pH and (2) whether zooxanthellate corals are more tolerant to low pH than azooxanthellate corals. To test these hypotheses, two Mediterranean colonial corals Cladocora caespitosa (zooxanthellate) and Astroides calycularis (azooxanthellate) were collected from CO2 vents and reference sites and incubated in the laboratory under present-day (pH on the total scale, pHT 8.07) and low pH conditions (pHT 7.70). Rates of net calcification, dark respiration and photosynthesis were monitored during a six-month experiment. Monthly net calcification was assessed every 27 to 35 d using the buoyant weight technique, whereas light and dark net calcification was estimated using the alkalinity anomaly technique during 1 h incubations. Neither species showed any change in net calcification rates, respiration, and photosynthesis regardless of their environmental history, pH treatment and trophic strategy. Our results indicate that C. caespitosa and A. calycularis could tolerate future ocean acidification conditions for at least 6 months. These results will aid in predicting species' future responses to ocean acidification, and thus improve the management and conservation of Mediterranean corals.
    Keywords: Alkalinity, total; Alkalinity, total, standard deviation; Animalia; Aragonite saturation state; Aragonite saturation state, standard deviation; Astroides calycularis; Benthic animals; Benthos; Bicarbonate ion; Bicarbonate ion, standard deviation; Calcification/Dissolution; Calcification rate, standard error; Calcification rate of calcium carbonate; Calcite saturation state; Calcite saturation state, standard deviation; Calculated using seacarb; Calculated using seacarb after Nisumaa et al. (2010); Calculated using seacarb after Orr et al. (2018); Carbon, inorganic, dissolved; Carbon, inorganic, dissolved, standard deviation; Carbonate ion; Carbonate ion, standard deviation; Carbonate system computation flag; Carbon dioxide; Carbon dioxide, standard deviation; Cladocora caespitosa; Cnidaria; Coast and continental shelf; Containers and aquaria (20-1000 L or 〈 1 m**2); Fugacity of carbon dioxide (water) at sea surface temperature (wet air); Fugacity of carbon dioxide in seawater, standard deviation; Gross photosynthesis rate, oxygen; Laboratory experiment; Mediterranean Sea; OA-ICC; Ocean Acidification International Coordination Centre; Origin; Partial pressure of carbon dioxide, standard deviation; Partial pressure of carbon dioxide (water) at sea surface temperature (wet air); pH; pH, standard deviation; Photosynthesis rate of oxygen, standard error; Potentiometric; Potentiometric titration; Primary production/Photosynthesis; Registration number of species; Replicates; Respiration; Respiration rate, oxygen; Respiration rate, oxygen, standard error; Salinity; Salinity, standard deviation; Single species; Species; Temperate; Temperature, water; Temperature, water, standard deviation; Treatment; Type; Uniform resource locator/link to reference
    Type: Dataset
    Format: text/tab-separated-values, 456 data points
    Location Call Number Limitation Availability
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  • 13
    Publication Date: 2024-03-15
    Description: The ability of coral populations to recover from disturbance depends on larval dispersion and recruitment. While ocean warming and acidification effects on adult corals are well documented, information on early life stages is comparatively scarce. Here, we investigate whether ocean warming and acidification can affect the larval and recruit development of the Mediterranean azooxanthellate coral Astroides calycularis. Larvae and recruits were raised for 9 months at ambient (23 ∘C) and warm (26 ∘C) temperatures and ambient (8.0) and low pH (7.7, on the total scale). The timing of the larval metamorphosis, growth of the recruit polyp by linear extension and budding, and skeletal characteristics of the 9-month-old polyps were monitored. Settlement and metamorphosis were more successful and hastened under a warm temperature. In contrast, low pH delayed the metamorphosis and affected the growth of the recruits by reducing the calcified area of attachment to the substrate as well as by diminishing the skeleton volume and the number of septa. However, skeleton density was higher under low pH and ambient temperature. The warm temperature and low-pH treatment had a negative impact on the survival, settlement, and growth of recruits. This study provides evidence of the threat represented by ocean warming and acidification for the larval recruitment and the growth of recruits of A. calycularis.
    Keywords: Age; Alkalinity, total; Alkalinity, total, standard deviation; Animalia; Aragonite saturation state; Aragonite saturation state, standard deviation; Astroides calycularis; Benthic animals; Benthos; Bicarbonate ion; Bottles or small containers/Aquaria (〈20 L); Calcite saturation state; Calcite saturation state, standard deviation; Calculated using seacarb; Calculated using seacarb after Nisumaa et al. (2010); Carbon, inorganic, dissolved; Carbon, inorganic, dissolved, standard deviation; Carbonate ion; Carbonate system computation flag; Carbon dioxide; Cnidaria; Coast and continental shelf; Colony number/ID; Condition; Date; Density; Development; Diameter; EXP; Experiment; Figure; Fugacity of carbon dioxide (water) at sea surface temperature (wet air); Growth/Morphology; Height; Identification; Laboratory experiment; Larvae, swimming; Length; Mediterranean Sea; Mortality/Survival; Number; Number of buds; OA-ICC; Ocean Acidification International Coordination Centre; Other studied parameter or process; Partial pressure of carbon dioxide, standard deviation; Partial pressure of carbon dioxide (water) at sea surface temperature (wet air); Pelagos; pH; pH, standard deviation; Polyp number; Potentiometric; Potentiometric titration; Replicates; Reproduction; Salinity; Salinity, standard deviation; Sant_Angelo; Single species; Species, unique identification; Species, unique identification (Semantic URI); Species, unique identification (URI); Surface area; Temperate; Temperature; Temperature, water; Temperature, water, standard deviation; Treatment; Type; Volume; Width; Zooplankton
    Type: Dataset
    Format: text/tab-separated-values, 208077 data points
    Location Call Number Limitation Availability
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  • 14
    Publication Date: 2024-03-15
    Description: Percent cover of 215 benthic species was quantified using two approaches with scuba diving: i) visual census techniques in Shallow Reef (benthic surveys were performed from 0.5 to 3 m depth) and Reef habitats (benthic surveys at ~10 m depth) dominated by algae and ii) photographic surveys in Caves (benthic surveys at ~3 m depth) and Deep Reef habitats (benthic surveys at ~ 40 m depth) dominated by sessile invertebrate animals. For visual censuses, 12 quadrats (25x25 cm) were haphazardly placed at Vent 1 and Vent 3 and the two corresponding ambient pH sites (n = 3 sites per habitat, n = 36 quadrats for Shallow Reefs and Reefs, respectively). For photographic surveys, 48–54 and 24 photoquadrats (25x25 cm) were taken at Vent 2 and Vent 4 and the two corresponding reference sites with ambient pH for each habitat (n = 3 sites per habitat, n = 144 photographic quadrats for Caves and n = 72 photographic quadrats for Deep Reefs, respectively). Both types of quadrats (visual censuses in the field and photographs in the lab) were divided into a grid of 25 squares (5 cm x 5 cm each). Percentage cover was quantified by counting the number of squares filled in the grid by the species and expressing the final values as relative percentages.
    Keywords: a1a_amb; a1b_amb; a2a_amb; a2b_amb; a3a_amb; a3b_amb; a4a_amb; a4b_amb; Alkalinity, total; Alkalinity, total, standard deviation; Aragonite saturation state; Aragonite saturation state, standard deviation; Benthos; Bicarbonate ion; Calcite saturation state; Calcite saturation state, standard deviation; Calculated using seacarb; Calculated using seacarb after Nisumaa et al. (2010); Carbon, inorganic, dissolved; Carbon, inorganic, dissolved, standard deviation; Carbonate ion; Carbonate system computation flag; Carbon dioxide; CO2 vent; Coast and continental shelf; Community composition and diversity; Condition; Coverage; Entire community; Event label; Field observation; Fugacity of carbon dioxide (water) at sea surface temperature (wet air); Mediterranean Sea; OA-ICC; Ocean Acidification International Coordination Centre; Partial pressure of carbon dioxide, standard deviation; Partial pressure of carbon dioxide (water) at sea surface temperature (wet air); pH; pH, standard deviation; Rocky-shore community; Salinity; Salinity, standard deviation; Sample code/label; Species; Temperate; Temperature, water; Temperature, water, standard deviation; Type of study; v1s_low; v2_low; v3_low; v4_low
    Type: Dataset
    Format: text/tab-separated-values, 110124 data points
    Location Call Number Limitation Availability
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  • 15
    Publication Date: 2024-03-15
    Description: We show here that CO2 partial pressure (pCO2) and temperature significantly interact on coral physiology. The effects of increased pCO2 and temperature on photosynthesis, respiration and calcification rates were investigated in the scleractinian coral Stylophora pistillata. Cuttings were exposed to temperatures of 25°C or 28°C and to pCO2 values of ca. 460 or 760 muatm for 5 weeks. The contents of chlorophyll c2 and protein remained constant throughout the experiment, while the chlorophyll a content was significantly affected by temperature, and was higher under the 'high-temperature-high-pCO2' condition. The cell-specific density was higher at 'high pCO2' than at 'normal pCO2' (1.7 vs. 1.4). The net photosynthesis normalized per unit protein was affected by both temperature and pCO2, whereas respiration was not affected by the treatments. Calcification decreased by 50% when temperature and pCO2 were both elevated. Calcification under normal temperature did not change in response to an increased pCO2. This is not in agreement with numerous published papers that describe a negative relationship between marine calcification and CO2. The confounding effect of temperature has the potential to explain a large portion of the variability of the relationship between calcification and pCO2 reported in the literature, and warrants a re-evaluation of the projected decrease of marine calcification by the year 2100.
    Keywords: Alkalinity, total; Animalia; Aragonite saturation state; Benthic animals; Benthos; Bicarbonate ion; Calcification/Dissolution; Calcification rate; Calcite saturation state; Calculated using seacarb after Nisumaa et al. (2010); Carbon, inorganic, dissolved; Carbonate ion; Carbonate system computation flag; Carbon dioxide; Cnidaria; Containers and aquaria (20-1000 L or 〈 1 m**2); DATE/TIME; EPOCA; Estimated; Estimated by regressing O2 against time; EUR-OCEANS; European network of excellence for Ocean Ecosystems Analysis; European Project on Ocean Acidification; Fugacity of carbon dioxide (water) at sea surface temperature (wet air); Identification; Laboratory experiment; Laboratory strains; Measured; PAR sensor LI-1000, LI-COR Inc.; Partial pressure of carbon dioxide (water) at sea surface temperature (wet air); pH; Salinity; Single species; Stylophora pistillata; Temperature; Temperature, water
    Type: Dataset
    Format: text/tab-separated-values, 1600 data points
    Location Call Number Limitation Availability
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  • 16
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    PANGAEA
    In:  Supplement to: Leclercq, Nicolas; Gattuso, Jean-Pierre; Jaubert, Jean (2000): CO2 partial pressure controls the calcification rate of a coral community. Global Change Biology, 6(3), 329-334, https://doi.org/10.1046/j.1365-2486.2000.00315.x
    Publication Date: 2024-03-15
    Description: Previous studies have demonstrated that coral and algal calcification is tightly regulated by the calcium carbonate saturation state of seawater. This parameter is likely to decrease in response to the increase of dissolved CO2 resulting from the global increase of the partial pressure of atmospheric CO2. We have investigated the response of a coral reef community dominated by scleractinian corals, but also including other calcifying organisms such as calcareous algae, crustaceans, gastropods and echinoderms, and kept in an open-top mesocosm. Seawater pCO2 was modified by manipulating the pCO2 of air used to bubble the mesocosm. The aragonite saturation state (omega arag) of the seawater in the mesocosm varied between 1.3 and 5.4. Community calcification decreased as a function of increasing pCO2 and decreasing omega arag. This result is in agreement with previous data collected on scleractinian corals, coralline algae and in a reef mesocosm, even though some of these studies did not manipulate CO2 directly. Our data suggest that the rate of calcification during the last glacial maximum might have been 114% of the preindustrial rate. Moreover, using the average emission scenario (IS92a) of the Intergovernmental Panel on Climate Change, we predict that the calcification rate of scleractinian-dominated communities may decrease by 21% between the pre-industrial period (year 1880) and the time at which pCO2 will double (year 2065).
    Keywords: Alkalinity, total; Aragonite saturation state; Benthos; Bicarbonate ion; Calcification/Dissolution; Calcification rate of calcium carbonate; Calcite saturation state; Calculated using seacarb after Nisumaa et al. (2010); Carbon, inorganic, dissolved; Carbonate ion; Carbonate system computation flag; Carbon dioxide; Containers and aquaria (20-1000 L or 〈 1 m**2); Entire community; EPOCA; EUR-OCEANS; European network of excellence for Ocean Ecosystems Analysis; European Project on Ocean Acidification; Experimental treatment; extracted from figure using GraphClick; Fugacity of carbon dioxide (water) at sea surface temperature (wet air); Laboratory experiment; Not applicable; OA-ICC; Ocean Acidification International Coordination Centre; Partial pressure of carbon dioxide (water) at sea surface temperature (wet air); pH; Rocky-shore community; Salinity; Temperature, water
    Type: Dataset
    Format: text/tab-separated-values, 1360 data points
    Location Call Number Limitation Availability
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  • 17
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    PANGAEA
    In:  Supplement to: Leclercq, Nicolas; Gattuso, Jean-Pierre; Jaubert, Jean (2002): Primary production, respiration, and calcification of a coral reef mesocosm under increased CO2 partial pressure. Limnology and Oceanography, 47(2), 558-564, https://doi.org/10.4319/lo.2002.47.2.0558
    Publication Date: 2024-03-15
    Description: The effect of increased CO2 partial pressure (pCO2) on the community metabolism (primary production, respiration, and calcification) of a coral community was investigated over periods ranging from 9 to 30 d. The community was set up in an open-top mesocosm within which pCO2 was manipulated (411, 647, and 918 µatm). The effect of increased pCO2 on the rate of calcification of the sand area of the mesocosm was also investigated. The net community primary production (NCP) did not change significantly with respect to pCO2 and was 5.1 ± 0.9 mmol O2 m-2 h-1, Dark respiration (R) increased slightly during the experiment at high pCO2, but this did not affect significantly the NCP:R ratio (1.0 ± 0.2). The rate of calcification exhibited the trend previously reported; it decreased as a function of increasing pCO2 and decreasing aragonite saturation state. This re-emphasizes the predictions that reef calcification is likely to decrease during the next century. The dissolution process of calcareous sand does not seem to be affected by open seawater carbonate chemistry; rather, it seems to be controlled by the biogeochemistry of sediment pore water.
    Keywords: Alkalinity, total; Alkalinity anomaly technique (Smith and Key, 1975); Aragonite saturation state; Benthos; Bicarbonate ion; Calcification/Dissolution; Calcification rate of calcium carbonate; Calcite saturation state; Calculated; Calculated using seacarb after Nisumaa et al. (2010); Carbon, inorganic, dissolved; Carbonate ion; Carbonate system computation flag; Carbon dioxide; Entire community; EPOCA; EUR-OCEANS; European network of excellence for Ocean Ecosystems Analysis; European Project on Ocean Acidification; Experimental treatment; Fugacity of carbon dioxide (water) at sea surface temperature (wet air); Laboratory experiment; Measured; Not applicable; OA-ICC; Ocean Acidification International Coordination Centre; Partial pressure of carbon dioxide (water) at sea surface temperature (wet air); pH; pH meter (Orion); Respiration; Rocky-shore community; Salinity; Site; Temperature, water
    Type: Dataset
    Format: text/tab-separated-values, 288 data points
    Location Call Number Limitation Availability
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  • 18
    Publication Date: 2024-03-15
    Keywords: Alkalinity, total; Aragonite saturation state; Bicarbonate ion; Calcite saturation state; Calculated using seacarb after Nisumaa et al. (2010); Carbon, inorganic, dissolved; Carbonate ion; Carbonate system computation flag; CTD, Sea-Bird, SBE 25 SEALOGGER; DEPTH, water; Fugacity of carbon dioxide (water) at sea surface temperature (wet air); Measured spectrophotometrically (Dickson et al., 2007) with purified m-cresol purple; MON; Monitoring; Partial pressure of carbon dioxide (water) at sea surface temperature (wet air); pH; Point_B; Salinity; Sampling date; Temperature, water; Titration potentiometric (SNAPO-CO2); Villefranche sur Mer, France
    Type: Dataset
    Format: text/tab-separated-values, 18816 data points
    Location Call Number Limitation Availability
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  • 19
    Publication Date: 2024-03-15
    Description: Predicting the potential for species adaption to climate change is challenged by the need to identify the physiological mechanisms that underpin species vulnerability. Here we investigated the sensitivity to ocean acidification in marine mussels during early development, and specifically the trochophore stage. Using RNA and DNA sequencing and in situ RNA hybridization, we identified developmental processes associated with abnormal development and rapid adaptation to low pH. Trochophores exposed to low pH seawater exhibited 43 differentially expressed genes. Gene annotation and in situ hybridization of differentially expressed genes point to pH sensitivity of (1) shell field development and (2) cellular stress response. Five genes within these two processes exhibited shifts in allele frequencies indicative of a potential for rapid adaptation. This case study contributes direct evidence that protecting species' existing genetic diversity is a critical management action to facilitate species resilience to climate change.
    Keywords: Accession number; Alkalinity, total; Alkalinity, total, standard deviation; Animalia; Aragonite saturation state; Bicarbonate ion; Calcite saturation state; Calculated using seacarb after Nisumaa et al. (2010); Carbon, inorganic, dissolved; Carbonate ion; Carbonate system computation flag; Carbon dioxide; Coast and continental shelf; Containers and aquaria (20-1000 L or 〈 1 m**2); EXP; Experiment; Fold change; Frequency; Fugacity of carbon dioxide (water) at sea surface temperature (wet air); Gene expression (incl. proteomics); Gene name; Laboratory experiment; Mediterranean Sea; Mollusca; Mytilus galloprovincialis; OA-ICC; Ocean Acidification International Coordination Centre; Partial pressure of carbon dioxide (water) at sea surface temperature (wet air); Pelagos; pH; Ratio; Replicates; Salinity; Salinity, standard deviation; Sample ID; Single species; Species, unique identification; Species, unique identification (Semantic URI); Species, unique identification (URI); Temperate; Temperature, water; Temperature, water, standard deviation; Threshold cycle, quantitative polymerase chain reaction; Time in days; Treatment; Type; Villefranche_sur_Mer; Zooplankton; Δ threshold cycle, quantitative polymerase chain reaction
    Type: Dataset
    Format: text/tab-separated-values, 10635 data points
    Location Call Number Limitation Availability
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  • 20
    Publication Date: 2024-03-15
    Description: We show here that CO2 partial pressure (pCO2) and temperature significantly interact on coral physiology. The effects of increased pCO2 and temperature on photosynthesis, respiration and calcification rates were investigated in the scleractinian coral Stylophora pistillata. Cuttings were exposed to temperatures of 25°C or 28°C and to pCO2 values of ca. 460 or 760 muatm for 5 weeks. The contents of chlorophyll c2 and protein remained constant throughout the experiment, while the chlorophyll a content was significantly affected by temperature, and was higher under the 'high-temperature-high-pCO2' condition. The cell-specific density was higher at 'high pCO2' than at 'normal pCO2' (1.7 vs. 1.4). The net photosynthesis normalized per unit protein was affected by both temperature and pCO2, whereas respiration was not affected by the treatments. Calcification decreased by 50% when temperature and pCO2 were both elevated. Calcification under normal temperature did not change in response to an increased pCO2. This is not in agreement with numerous published papers that describe a negative relationship between marine calcification and CO2. The confounding effect of temperature has the potential to explain a large portion of the variability of the relationship between calcification and pCO2 reported in the literature, and warrants a re-evaluation of the projected decrease of marine calcification by the year 2100.
    Keywords: Alkalinity, total; Animalia; Aragonite saturation state; Benthic animals; Benthos; Bicarbonate ion; Biomass/Abundance/Elemental composition; Calcification/Dissolution; Calcification rate; Calcite saturation state; Calculated from skeletal dry weight; Calculated using seacarb after Nisumaa et al. (2010); Carbon, inorganic, dissolved; Carbonate ion; Carbonate system computation flag; Carbon dioxide; Cnidaria; Containers and aquaria (20-1000 L or 〈 1 m**2); Counting; Density, cell-specific; EPOCA; Estimated; Estimated by regressing O2 against time; EUR-OCEANS; European network of excellence for Ocean Ecosystems Analysis; European Project on Ocean Acidification; EXP; Experiment; Experimental treatment; Fugacity of carbon dioxide (water) at sea surface temperature (wet air); Laboratory experiment; Laboratory strains; Measured; Net photosynthesis rate; PAR sensor LI-1000, LI-COR Inc.; Partial pressure of carbon dioxide (water) at sea surface temperature (wet air); pH; Primary production/Photosynthesis; Respiration; Respiration rate, oxygen; Reynaud_etal_03; Salinity; Single species; Stylophora pistillata; Temperature; Temperature, water
    Type: Dataset
    Format: text/tab-separated-values, 76 data points
    Location Call Number Limitation Availability
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