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  • 1
    Electronic Resource
    Electronic Resource
    The Murine T Cell Antigen Receptor and Associated Structures (1984)
    Oxford, UK : Blackwell Publishing Ltd
    Immunological reviews 81 (1984), S. 0 
    Details
    ISSN: 1600-065X
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Medicine
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1600-065X.1984.tb01108.x
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  • 2
    Electronic Resource
    Electronic Resource
    Multidimensional flow-cytometric analysis of dendritic cells in peripheral blood of normal donors and cancer patients (1997)
    Springer
    Cancer immunology immunotherapy 45 (1997), S. 234-240 
    Details
    ISSN: 1432-0851
    Keywords: Key words Dendritic cells ; Flow cytometry ; Stem cell transplantation ; Breast cancer
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract  We studied the potential of multidimensional flow cytometry to evaluate the frequency and maturation/activation status of dendritic cells in minimally manipulated peripheral blood mononuclear cell preparations (i.e., only separated on Ficoll-Hypaque) of normal donors and cancer patients. A rare subset of HLA-DR+ leukocytes (less than 1% mononuclear cells) was detected in blood of normal donors that displayed all the features of dendritic cells: these cells had high forward-light-scatter characteristics and coexpressed CD4, CD86 and CD54 surface antigens, but lacked the lineage-associated surface markers of T cells, B cells, monocytes, granulocytes or NK i.e. they were CD3–, CD19–, CD20–, CD14–, CD11b–, CD16–, CD56–). These physical and phenotypic properties were virtually identical to those of immunomagnetically sorted leukocytes characterized as dendritic-cells on the basis of morphology, phenotype and high stimulatory activity in allogeneic mixed-lymphocyte cultures. Using this flow-cytometric approach we observed that the frequency of dendritic cell-like cells in peripheral blood mononuclear cell specimens of cancer patients receiving chemotherapy alone or those recovering from stem cell transplantation was significantly lower than that of normal individuals (mean ± SE: 0.36 ± 0.05%, 0.14 ± 0.06%, and 0.75 ± 0.04% respectively). Multidimensional flow-cytometric analysis of dendritic cells might represent an important new tool for assessing immunocompetence, and for monitoring the effects of therapeutic regimens on the immune system.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s002620050438
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  • 3
    Electronic Resource
    Electronic Resource
    Expression and ligand-binding function of the integrin α4β1 (VLA-4) on neural-crest-derived tumor cell lines (1992)
    Springer
    Clinical & experimental metastasis 10 (1992), S. 281-290 
    Details
    ISSN: 1573-7276
    Keywords: α4β1 ; fibronectin ; integrins ; neural tumor
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract Human neural-crest-derived tumor cell lines, including three neuroblastomas, an astrocytoma, a glioblastoma, a rhabdomyosarcoma and a melanoma were screened for the expression of the integrin α4β1 (VLA-4). The neuroblastomas IMR-32 and SK-N-SH, the astrocytoma 131-INI, the glioblastoma Fogerty and the rhabdomyosarcoma TE-671 expressed α4β1 as determined by cytofluorometry and immunoprecipitation. Another neuroblastoma line, LA-N-1, did not express α4β1. Analysis of immunoprecipitated α4β1 showed that the α4 subunit from the various cell types differed in relative molecular weight (M r ). The variability in the observed M r could be accounted for by differences in the levels of N-linked glycosylation. The observed variability in M r did not appear to affect function since intact cells and solubilized α4β1 bound to a synthetic peptide identical in sequence to the CS-1 region of the alternatively spliced IIICS domain of fibronectin, a known α4β1, ligand.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00133564
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  • 4
    Electronic Resource
    Electronic Resource
    Angiogenesis induced by tumor necrosis factor-agr; is mediated by α4 integrins (1998)
    Springer
    Angiogenesis 2 (1998), S. 265-275 
    Details
    ISSN: 1573-7209
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract Tumor necrosis factor-α (TNF-α) and fibroblast growth factor-2 (FGF-2 or bFGF) are potent stimulators of angiogenesis. TNF-α, but not FGF-2, can induce the expression of vascular cell adhesion molecule-1 (VCAM-1) on the surface of endothelial cells. The soluble form of VCAM-1 has recently been demonstrated to function as an angiogenic mediator. Here we demonstrate that monoclonal antibodies directed against VCAM-1 or its α4 integrin counter-receptor inhibited TNF-α-induced endothelial cell migration in vitro. Angiogenesis induced in vivo in rat corneas by TNF-α was inhibited by a neutralizing antibody directed against the rat α4 integrin subunit. A peptide antagonist of the a4 integrins blocked TNF-α-induced endothelial cell migration in vitro and angiogenesis in rat corneas in vivo. No inhibition by the antibodies or peptide antagonist was observed either in vitro or in vivo when FGF-2 was used as the stimulus. The peptide antagonist did not inhibit TNF-a binding to its receptor nor did it block the function of αvβ3, an integrin previously implicated in TNF-a and FGF-2 mediated angiogenesis. These results demonstrate that angiogenic processes induced by TNF-α are mediated in part by agr;4 integrins possibly by a mechanism involving the induction of soluble VCAM-1.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1023/A:1009296700991
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  • 5
    Electronic Resource
    Electronic Resource
    Integrin-mediated entry into S phase of human gastric adenocarcinoma cells (1995)
    Springer
    Clinical & experimental metastasis 13 (1995), S. 427-438 
    Details
    ISSN: 1573-7276
    Keywords: adhesion ; α2 integrin ; β1 integrin ; cell cycle ; collagen
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract The integrins are a family of integral membrane receptors that participate in binding to various extracellular and cell surface proteins during adhesion, migration, and homing of normal and neoplastic cells. In this study, we characterized the involvement of integrins in mediating the growth of an adhesion-dependent gastric adenocarcinoma line, ST2. This line was distinguished and selected for study based on its inability to grow when suspended in soft agar or plated on poly(2-hydroxyethyl methacrylate)-coated dishes. ST2 cells arrested in G0/G1 of the cell cycle when deprived of adhesion to substrate. Using purified matrix components, collagen was found to be highly active in promoting β1 integrin-mediated cell attachment and spreading. Subsequent to spreading on collagen, the cells were released from G0/G1 block and progressed into S phase. Monoclonal antibodies to α2 or β1 integrin blocked the reinduction of both cell spreading and entry into S phase. These studies suggest that during the metastatic process, integrin receptor interaction with the insoluble matrix may be an important step leading to proliferation of some tumors.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00118182
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  • 6
    Electronic Resource
    Electronic Resource
    Triggering of the proteinase dipeptidyl peptidase IV (CD26) amplifies human T lymphocyte proliferation (1991)
    New York, N.Y. : Wiley-Blackwell
    Journal of Cellular Biochemistry 46 (1991), S. 206-218 
    Details
    ISSN: 0730-2312
    Keywords: T cell proliferation ; enzyme overlay membrane ; T cell proteinase ; Ta1 ; memory ; Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Chemistry and Pharmacology , Medicine
    Notes: CD26 (Ta1, dipeptidyl peptidase IV) is a Mr 105,000 protein expressed at high levels on activated T lymphocytes and is a potential marker of memory T cells. Reciprocal immunodepletion and solid phase double determinant binding studies showed that mAb AC7 and the CD26-specific mAb anti-Ta1 reacted with spatially distinct sites on the same molecule. The proteinase dipeptidyl peptidase IV (DPP IV) was immunoprecipitated with mAb AC7 and its enzymatic activity directly assayed using an enzyme overlay membrane system. High levels of DPP IV activity were detected on the T cell tumor line CCRF-HSB-2 and on PBMC stimulated by a variety of methods. By itself, soluble mAb AC7 was not mitogenic for T cells but enhanced T cell proliferation that resulted from treatment with phorbol myristic acetate (PMA) in the presence of accessory cells. T cell proliferation was also induced by co-immobilized mAb AC7 and mAb OKT3 (anti-CD3). Cultures of T cells growing in the presence of IL-2 responded with accelerated growth when exposed to a combination of immobilized mAb AC7 and soluble mAb OKT3, a result not seen with freshly isolated T cells.
    Additional Material: 5 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/jcb.240460304
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