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  • 1
    Electronic Resource
    Electronic Resource
    Multidimensional flow-cytometric analysis of dendritic cells in peripheral blood of normal donors and cancer patients (1997)
    Springer
    Cancer immunology immunotherapy 45 (1997), S. 234-240 
    Details
    ISSN: 1432-0851
    Keywords: Key words Dendritic cells ; Flow cytometry ; Stem cell transplantation ; Breast cancer
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract  We studied the potential of multidimensional flow cytometry to evaluate the frequency and maturation/activation status of dendritic cells in minimally manipulated peripheral blood mononuclear cell preparations (i.e., only separated on Ficoll-Hypaque) of normal donors and cancer patients. A rare subset of HLA-DR+ leukocytes (less than 1% mononuclear cells) was detected in blood of normal donors that displayed all the features of dendritic cells: these cells had high forward-light-scatter characteristics and coexpressed CD4, CD86 and CD54 surface antigens, but lacked the lineage-associated surface markers of T cells, B cells, monocytes, granulocytes or NK i.e. they were CD3–, CD19–, CD20–, CD14–, CD11b–, CD16–, CD56–). These physical and phenotypic properties were virtually identical to those of immunomagnetically sorted leukocytes characterized as dendritic-cells on the basis of morphology, phenotype and high stimulatory activity in allogeneic mixed-lymphocyte cultures. Using this flow-cytometric approach we observed that the frequency of dendritic cell-like cells in peripheral blood mononuclear cell specimens of cancer patients receiving chemotherapy alone or those recovering from stem cell transplantation was significantly lower than that of normal individuals (mean ± SE: 0.36 ± 0.05%, 0.14 ± 0.06%, and 0.75 ± 0.04% respectively). Multidimensional flow-cytometric analysis of dendritic cells might represent an important new tool for assessing immunocompetence, and for monitoring the effects of therapeutic regimens on the immune system.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s002620050438
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  • 2
    Electronic Resource
    Electronic Resource
    Extracellular iron reductase activity produced by Listeria monocytogenes (1996)
    Springer
    Archives of microbiology 166 (1996), S. 51-57 
    Details
    ISSN: 1432-072X
    Keywords: Key words Iron ; Listeria monocytogenes ; Extracellular iron reduction ; NADH:iron reductase
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Little is known about how pathogenic microorganisms that do not produce low-molecular-weight iron-chelating agents, termed siderophores, acquire iron from their environment. We have identified an extracellular enzyme produced by Listeria monocytogenes that can mobilize iron from a variety of iron-chelate complexes via reduction of the metal. The iron reductase requires Mg2+, flavin mononucleotide (FMN), and reduced nicotinamide adenine dinucleotide (NADH) for activity. Saturation kinetics were found when initial velocity studies of iron reduction were carried out as a function of variable FMN concentrations in the presence of 100 μM NADH and 10 mM Mg2+. Hyperbolic kinetics were also found when these studies were repeated as a function of variable NADH concentrations along with 20 μM FMN and 10 mM Mg2+. This process of extracellular reduction, in all likelihood, could be involved in the mobilization of iron from soils and aqueous environments and from host tissues in pathogenic processes. This is the first report of the extracellular enzymic reduction of iron by microorganisms.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s002030050354
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  • 3
    Electronic Resource
    Electronic Resource
    Adherence of a virulent strain of Listeria monocytogenes to the surface of a hepatocarcinoma cell line via lectin-substrate interaction (1990)
    Springer
    Archives of microbiology 153 (1990), S. 282-286 
    Details
    ISSN: 1432-072X
    Keywords: Adherence ; Attachment ; Bacterial penetration ; Cell-cell interactions ; d-galactose ; Invasion ; Lectin ; Listeria monocytogenes ; Surface carbohydrate ; Virulence factor
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Listeria monocytogenes was examined for the presence of surface carbohydrates to ascertain whether surface sugars, if present, would interact with eucaryotic surface carbohydrate receptors. We found that a virulent, but not two avirulent strains had a surface α-d-galactose residue as determined by agglutination with Griffonia simplicifolia (GS-I) and other lectins. The virulent strain bound to a human hepatocarcinoma cell line (HepG2), which has a well characterized receptor for α-d-galactose. This interaction could be blocked by pretreatment of the HepG2 cells with either α-d-galactose or neuraminidase, the latter of which will render the galactose receptor functionally inactive. We propose that the attachment of the virulent Listeria to eucaryotic cells occurs as a result of the interaction of the microbial α-d-galactose with that of the eucaryotic galactose receptor. This surface carbohydrate may provide an explanation for the mechanism of attachment and penetration of virulent Listeria into host cells during infection. As such, this may allow for amplication of pathogenesis through intracellular multiplication in nonprofessional phagocytes prior to macrophage involvement.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00249083
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  • 4
    Electronic Resource
    Electronic Resource
    The role of iron in the production of hemolysin byListeria monocytogenes (1981)
    Springer
    Current microbiology 6 (1981), S. 287-290 
    Details
    ISSN: 1432-0991
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Abstract The in vitro production of hemolysin byListeria monocytogenes, a facultative intracellular bacterial pathogen, was examined as a function of medium iron. There was an inverse relationship between iron and hemolysin production: Hemolysis increased as medium iron concentration decreased. This study offers the first evidence that the in vitro production of hemolysin byListeria monocytogenes is under the control of external iron in growth medium.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01566878
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