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  • 1
    ISSN: 1460-9568
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Medicine
    Notes: [3H]-Methyllycaconitine ([3H]-MLA) is a new radioligand with selectivity for α7-type neuronal nicotinic acetylcholine receptors (nAChRs). In our previous study [Davies, A.R.L., Hardick, D.J., Blagbrough, I.S., Potter, B.V.L., Wolstenholme, A.J. & Wonnacott, S. (1999) Neuropharmacology, 38, 679–690], this radioligand labelled a single class of site in rat brain membranes; its pharmacology and distribution in crudely dissected brain regions closely paralleled that of the well-established α7-ligand [125I]-α-bungarotoxin. However, a small population of [3H]-MLA binding sites was apparently insensitive to α-bungarotoxin. Here we have extended the study to mouse brain, using autoradiography to examine the distribution of [3H]-MLA and [125I]-α-bungarotoxin binding sites. [3H]-MLA labelled a single class of site in mouse brain membranes with a KD of 2.2 n m and a Bmax of 45.6 fmol/mg protein. Specific binding, defined by unlabelled MLA (Ki = 0.69 n m), was completely inhibited by (–)-nicotine (Ki = 1.62 μm), whereas α-bungarotoxin inhibited only 85% of specific binding (Ki = 3.5 n m). The distributions of [125I]-α-bungarotoxin and [3H]-MLA binding sites were compared by autoradiography, and binding was quantitated in 72 brain regions. Binding of both radioligands was highly correlated, with highest densities in the dorsal tegmental nucleus of the pons, colliculi and hippocampus. Serial sections labelled with [3H]-MLA in the absence or presence of unlabelled MLA or α-bungarotoxin provided no evidence for any α-bungarotoxin-resistant binding. The results are discussed in terms of binding sites that are inaccessible to α-bungarotoxin in membrane preparations. This study demonstrates the utility of [3H]-MLA for characterization of α7-type nicotinic receptors in mammalian brain, and suggests that it labels a population identical to that defined by [125I]-α-bungarotoxin.
    Type of Medium: Electronic Resource
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  • 2
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    London : Periodicals Archive Online (PAO)
    Medical History. 17 (1973) 386 
    ISSN: 0025-7273
    Topics: History , Medicine
    Description / Table of Contents: News, Notes and Queries
    Notes: OCTOBER
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  • 3
    Electronic Resource
    Electronic Resource
    New York, NY : Wiley-Blackwell
    BioEssays 18 (1996), S. 841-845 
    ISSN: 0265-9247
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Medicine
    Notes: The mechanisms responsible for the fine tuning of development, where the wildtype phenotype is reproduced with high fidelity, are not well understood. The difficulty in approaching this problem is the identification of mutant phenotypes indicative of a defect in these fine-tuning control mechanisms. Evolutionary biologists have used asymmetry as a measure of developmental homeostasis. The rationale for this was that, since the same genome controls the development of the left and right sides of a bilaterally symmetrical organism, departures from symmetry can be used to measure genetic or environmental perturbations. This paper examines the relationship between asymmtry and resistance to organophosphorous insecticides in the Australian sheep blowfly, Lucilia cuprina. A resistance gene, Rop-1, which encodes a carboxylesterase enzyme, also confers a significant increase in asymmetry. Continued exposure of resistant populations to insecticide has selected a dominant suppressor of the asymmetry phenotype. Genetic evidence indicates that the modifier is the L. cuprina Notch homologue.
    Additional Material: 1 Tab.
    Type of Medium: Electronic Resource
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  • 4
    Publication Date: 2020-07-01
    Description: Ocean acidification (OA) poses a major threat to marine animals, especially marine shelled invertebrates such as molluscs. Although many organisms are capable of compensating for the effects of OA, this can impose physiological costs and impact performance (e.g. through increased metabolism and decreased growth). Sublethal injuries on shells may provoke changes in energy allocation. Under acidified conditions, organisms would spend less energy on reproduction and somatic growth to repair the damage. Therefore, we analysed the physiological responses of the intertidal gastropod Tritia reticulata during shell regeneration under OA conditions. We simulated a sub-lethal predation event (a notch in the outer lip of the shell) and individuals were exposed to control (pH 8.08) and low pH scenarios (pH 7.88 and 7.65). After two months exposure, all individuals showed shell repair, with a full repair rate observed in 75% of individuals. Contrary to expectations, shell repair following sub-lethal damage and OA had no apparent impact on physiological state in terms of energy reserves (as measured by whole-animal Carbon/Nitrogen) or growth potential (as measured by whole-animal RNA:Protein and RNA:DNA ratios). As an intertidal organism, T. reticulata could be resilient to future global environmental change because of compensatory mechanisms that are inherent in intertidal animals, and may represent a robust species with which to study future scenarios of OA in temperate coastal ecosystems. However, unrestricted food availability during experiment could have played a role in the results and therefore food limitation should be considered in future studies regarding shell repair and metabolism under the effects of OA.
    Repository Name: EPIC Alfred Wegener Institut
    Type: Article , isiRev
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  • 5
    Publication Date: 2014-10-07
    Description: An important aim of large, pan-European scientific projects with numerous research groups is to integrate and visualize the acquired distributed data sets and results. The large volume of diverse data gathered and the need to disseminate results among the scientific community and beyond requires using a Geographic Information System (GIS). This article presents our experiences in creating a unified Web-based GIS for HERMES. The HERMES-GIS is based on Web Mapping Services that include direct links to the World Data Center for Marine Environmental Science and its large, long-term geoscience data archive and publication unit, PANGAEA (http://www.pangaea.de). It incorporates metadata and data from all project partners to provide users with basic analytical and visualization tools for archived (distributed) and personal (local) data, and it is also a policymaking tool. Additionally, we illustrate two important GIS applications inside the HERMES communitythe use of data models to integrate several subdisciplines and the use of predictive habitat modeling.
    Repository Name: EPIC Alfred Wegener Institut
    Type: Article , isiRev
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  • 6
    Publication Date: 2022-06-07
    Description: The Author(s), 2022. This article is distributed under the terms of the Creative Commons Attribution License. The definitive version was published in Rabe, B., Heuze, C., Regnery, J., Aksenov, Y., Allerholt, J., Athanase, M., Bai, Y., Basque, C., Bauch, D., Baumann, T. M., Chen, D., Cole, S. T., Craw, L., Davies, A., Damm, E., Dethloff, K., Divine, D., Doglioni, F., Ebert, F., Fang, Y-C., Fer, I., Fong, A. A., Gradinger, R., Granskog, M. A., Graupner, R., Haas, C., He, H., He, Y., Hoppmann, M., Janout, M., Kadko, D., Kanzow, T., Karam, S., Kawaguchi, Y., Koenig, Z., Kong, B., Krishfield, R. A., Krumpen, T., Kuhlmey, D., Kuznetsov, I., Lan, M., Laukert, G., Lei, R., Li, T., Torres-Valdés, S., Lin, L,. Lin, L., Liu, H., Liu, N., Loose, B., Ma, X., MacKay, R., Mallet, M., Mallett, R. D. C., Maslowski, W., Mertens, C., Mohrholz, V., Muilwijk, M., Nicolaus, M., O’Brien, J. K., Perovich, D., Ren, J., Rex, M., Ribeiro, N., Rinke, A., Schaffer, J., Schuffenhauer, I., Schulz, K., Shupe, M. D., Shaw, W., Sokolov, V., Sommerfeld, A., Spreen, G., Stanton, T., Stephens, M., Su, J., Sukhikh, N., Sundfjord, A., Thomisch, K., Tippenhauer, S., Toole, J. M., Vredenborg, M., Walter, M., Wang, H., Wang, L., Wang, Y., Wendisch, M., Zhao, J., Zhou, M., & Zhu, J. Overview of the MOSAiC expedition: physical oceanography. Elementa: Science of the Anthropocene, 10(1), (2022): 1, https://doi.org/10.1525/elementa.2021.00062.
    Description: Arctic Ocean properties and processes are highly relevant to the regional and global coupled climate system, yet still scarcely observed, especially in winter. Team OCEAN conducted a full year of physical oceanography observations as part of the Multidisciplinary drifting Observatory for the Study of the Arctic Climate (MOSAiC), a drift with the Arctic sea ice from October 2019 to September 2020. An international team designed and implemented the program to characterize the Arctic Ocean system in unprecedented detail, from the seafloor to the air-sea ice-ocean interface, from sub-mesoscales to pan-Arctic. The oceanographic measurements were coordinated with the other teams to explore the ocean physics and linkages to the climate and ecosystem. This paper introduces the major components of the physical oceanography program and complements the other team overviews of the MOSAiC observational program. Team OCEAN’s sampling strategy was designed around hydrographic ship-, ice- and autonomous platform-based measurements to improve the understanding of regional circulation and mixing processes. Measurements were carried out both routinely, with a regular schedule, and in response to storms or opening leads. Here we present along-drift time series of hydrographic properties, allowing insights into the seasonal and regional evolution of the water column from winter in the Laptev Sea to early summer in Fram Strait: freshening of the surface, deepening of the mixed layer, increase in temperature and salinity of the Atlantic Water. We also highlight the presence of Canada Basin deep water intrusions and a surface meltwater layer in leads. MOSAiC most likely was the most comprehensive program ever conducted over the ice-covered Arctic Ocean. While data analysis and interpretation are ongoing, the acquired datasets will support a wide range of physical oceanography and multi-disciplinary research. They will provide a significant foundation for assessing and advancing modeling capabilities in the Arctic Ocean.
    Description: The following projects and funding agencies contributed to this work: Why is the deep Arctic Ocean Warming is funded by the Swedish Research Council, project number 2018-03859, and berth fees for this project were covered by the Swedish Polar Research Secretariat; The Changing Arctic Ocean (CAO) program, jointly funded by the United Kingdom Research and Innovation (UKRI) Natural Environment Research Council (NERC) and the Bundesministerium für Bildung und Forschung (BMBF), in particular, the CAO projects Advective Pathways of nutrients and key Ecological substances in the ARctic (APEAR) grants NE/R012865/1, NE/R012865/2, and #03V01461, and the project Primary productivity driven by Escalating Arctic NUTrient fluxeS grant #03F0804A; The Research Council of Norway (AROMA, grant no 294396; HAVOC, grant no 280292; and CAATEX, grant no 280531); Collaborative Research: Thermodynamics and Dynamic Drivers of the Arctic Sea Ice Mass Budget at Multidisciplinary drifting Observatory for the Study of the Arctic Climate; National Science Foundation (NSF) projects 1723400, Stanton; OPP-1724551, Shupe; The Helmholtz society strategic investment Frontiers in Arctic Marine monitoring (FRAM); Deutsche Forschungsgemeinschaft (German Research Foundation) through the Transregional Collaborative Research Centre TRR 172 “ArctiC Amplification: Climate Relevant Atmospheric and SurfaCe Processes, and Feedback Mechanisms (AC)3” (grant 268020496); The Japan Society for the Promotion of Science (grant numbers JP18H03745, JP18KK0292, and JP17KK0083) and the COLE grant of U. Tokyo; National Key Research and Development Plan Sub-Project of Ministry of Science and Technology of China (2016YFA0601804), “Simulation, Prediction and Regional Climate Response of Global Warming Hiatus”, 2016/07-2021/06; National Science Foundation grant number OPP-1756100 which funded two of the Ice-Tethered Profilers and all the Ice-Tethered Profiler deployments; Chinese Polar Environmental Comprehensive Investigation and Assessment Programs, funded by the Chinese Arctic and Antarctic Administration; Marine Science and Technology Fund of Shandong Province for Qingdao National Laboratory for Marine Science and Technology (Grant: 2018SDKJ0104-1) and Chinese Natural Science Foundation (Grant: 41941012); UK NERC Long-term Science Multiple Centre National Capability Programme “North Atlantic Climate System Integrated Study (ACSIS)”, grant NE/N018044/1; The London NERC Doctoral Training Partnership grant (NE/L002485/1) which funded RDCM; NSF grant number OPP-1753423, which funded the 7Be tracer –measurements; and The Alfred-Wegener-Institut Helmholtz-Zentrum für Polar- und Meeresforschung (AWI) through its projects: AWI_OCEAN, AWI_ROV, AWI_ICE, AWI_SNOW, AWI_ECO, AWI_ATMO, and AWI_BGC.
    Keywords: Physical oceanography ; MOSAiC ; Arctic ; Coupled ; Drift ; Sea ice
    Repository Name: Woods Hole Open Access Server
    Type: Article
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  • 7
    Publication Date: 2024-04-23
    Repository Name: EPIC Alfred Wegener Institut
    Type: Article , peerRev
    Format: application/pdf
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  • 8
    Publication Date: 2012-01-01
    Description: Changes in the possible behavior of El Niño–Southern Oscillation (ENSO) with global warming have provoked interest in records of ENSO from past “greenhouse” climate states. The latest Cretaceous laminated Marca Shale of California permits a seasonal-scale reconstruction of water column flux events and hence interannual paleoclimate variability. The annual flux cycle resembles that of the modern Gulf of California with diatoms characteristic of spring upwelling blooms followed by silt and clay, and is consistent with the existence of a paleo–North American Monsoon that brought input of terrigenous sediment during summer storms and precipitation runoff. Variation is also indicated in the extent of water column oxygenation by differences in lamina preservation. Time series analysis of interannual variability in terrigenous sediment and diatom flux and in the degree of bioturbation indicates strong periodicities in the quasi-biennial (2.1–2.8 yr) and low-frequency (4.1–6.3 yr) bands both characteristic of ENSO forcing, as well as decadal frequencies. This evidence for robust Late Cretaceous ENSO variability does not support the theory of a “permanent El Niño,” in the sense of a continual El Niño–like state, in periods of warmer climate.
    Print ISSN: 0091-7613
    Electronic ISSN: 1943-2682
    Topics: Geosciences
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  • 9
    Publication Date: 2015-02-08
    Description: Article The role of cohesion is vital to our understanding of how sedimentary bedforms evolve. Here, the authors show that microorganisms within the sediment affect cohesion and demonstrate that ripples can take up to one hundred times as long to develop when extracellular polymeric substances are present. Nature Communications doi: 10.1038/ncomms7257 Authors: Jonathan Malarkey, Jaco H. Baas, Julie A. Hope, Rebecca J. Aspden, Daniel R. Parsons, Jeff Peakall, David M. Paterson, Robert J. Schindler, Leiping Ye, Ian D. Lichtman, Sarah J. Bass, Alan G. Davies, Andrew J. Manning, Peter D. Thorne
    Electronic ISSN: 2041-1723
    Topics: Biology , Chemistry and Pharmacology , Natural Sciences in General , Physics
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  • 10
    Publication Date: 2016-09-17
    Description: Sequence element enrichment analysis to determine the genetic basis of bacterial phenotypes Nature Communications, Published online: 16 September 2016; doi:10.1038/ncomms12797 Plasticity and clonal population structure in bacterial genomes can hinder traditional SNP-based genetic association studies. Here, Corander and colleagues present a method to identify variable-length sequence elements enriched in a phenotype of interest, and demonstrate its use in human pathogens.
    Electronic ISSN: 2041-1723
    Topics: Biology , Chemistry and Pharmacology , Natural Sciences in General , Physics
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