In:
Cold Spring Harbor Protocols, Cold Spring Harbor Laboratory, Vol. 2012, No. 3 ( 2012-03), p. pdb.prot067967-
Abstract:
For some applications, such as genome sequencing and high-throughput genotyping with multiple markers, it is necessary to use high-quality genomic DNA. This article describes how to obtain several micrograms of high-quality, cesium chloride-purified DNA from 1 g of Ectocarpus filaments. We also recommend using DNA of this quality for quantitative RT–PCR control reactions. However, simpler, more rapid, kit-based methods are preferable for experiments that involve the treatment of large numbers of individuals, such as genotyping large populations with a small number of markers or PCR screening of large populations.
Type of Medium:
Online Resource
ISSN:
1940-3402
,
1559-6095
DOI:
10.1101/pdb.prot067967
Language:
English
Publisher:
Cold Spring Harbor Laboratory
Publication Date:
2012