Extraction of High-Quality Genomic DNA from Ectocarpus
- Susana M. Coelho1,2,4,
- Delphine Scornet1,2,
- Sylvie Rousvoal1,2,
- Nick Peters1,2,
- Laurence Dartevelle1,2,
- Akira F. Peters2,3 and
- J. Mark Cock1,2
- 1UPMC Université Paris 06, The Marine Plants and Biomolecules Laboratory, UMR 7139, Station Biologique de Roscoff, 29682 Roscoff Cedex, France
- 2CNRS, UMR 7139, Laboratoire International Associé Dispersal and Adaptation in Marine Species, Station Biologique de Roscoff, 29682 Roscoff Cedex, France
- 3Bezhin Rosko, 29250 Santec, France
Abstract
For some applications, such as genome sequencing and high-throughput genotyping with multiple markers, it is necessary to use high-quality genomic DNA. This article describes how to obtain several micrograms of high-quality, cesium chloride-purified DNA from 1 g of Ectocarpus filaments. We also recommend using DNA of this quality for quantitative RT–PCR control reactions. However, simpler, more rapid, kit-based methods are preferable for experiments that involve the treatment of large numbers of individuals, such as genotyping large populations with a small number of markers or PCR screening of large populations.
Footnotes
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↵4 Correspondence: coelho{at}sb-roscoff.fr
- © 2012 Cold Spring Harbor Laboratory Press
