Description: The variability of the Atlantic Meridional Overturning Circulation (AMOC) has considerable impacts on the global climate system. Past studies have shown that changes in the South Atlantic control the stability of the AMOC and drive an important part of its variability. That is why significant resources have been invested in a South (S)AMOC observing system. In January 2017, the RV Maria S. Merian conducted the first GO‐SHIP hydrographic transect along the SAMOC‐Basin Wide Array (SAMBA) line at 34.5°S in the South Atlantic. This paper presents estimates of meridional volume, freshwater (MFT), and heat (MHT) transports through the line using the slow varying geostrophic density field and direct velocity observations. An upper and an abyssal overturning cell are identified with a strength of 15.64 ± 1.39 Sv and 2.4 ± 1.6 Sv, respectively. The net northward MHT is 0.27 ± 0.10 PW, increasing by 0.12 PW when we remove the observed mesoscale eddies with a climatology derived from the Argo floats data set. We attribute this change to an anomalous predominance of cold core eddies during the cruise period. The highest velocities are observed in the western boundary, within the Brazil and the Deep Western Boundary currents. These currents appear as a continuous deep jet located 150 km off the slope squeezed between two cyclonic eddies. The zonal changes in water masses properties and velocity denote the imprint of exchange pathways with both the Southern and the Indian oceans. Key Points: ● Overturning maximum is 15.64 ± 1.39 Sv; Meridional heat and freshwater transport are 0.27 ± 0.10 PW and 0.23 ± 0.02 Sv, respectively ● Excluding the mesoscale eddies from the section increased the meridional heat transport by 0.12 PW ● The distribution of water masses and currents reflects the favorable position of the section for observing