Publikationsdatum:
2024-03-08
Beschreibung:
We simulated an experimental summer storm in large-volume (~1200 m3, ~16m depth) enclosures in Lake Stechlin (https://www.lake-lab.de) by mixing deeper water masses from the meta- and hypolimnion into the mixed layer (epilimnion). The mixing included the disturbance of a deep chlorophyll maximum (DCM) which was present at the same time of the experiment in Lake Stechlin and situated in the metalimnion of each enclosure during filling. Size-fractionated Bacterial Protein Production (BPP) of particle associated (PA, 〉3.0 µm) and free-living bacteria (FL, 0.2-3.0 µm) (14C-Leu incorporation) as well as abundances of PA (microscopy of DAPI stained cells on 3.0 µm polycarbonate filters) and FL heterotrophic prokaryotes and picocyanobacteria (flow cytometry of SYBR green I stained cells) were monitored for 42 days after the experimental disturbance event. Mixing increased bacterial abundance and production about 3 weeks after mixing, which was associated to a mixing-induced stimulation of phytoplankton growth in the mixed enclosures compared to the controls. Simultaneously, decreased abundances of picocyanobacteria could be observed in mixed enclosures.
Schlagwort(e):
14C-leucine incorporation; Bacterial abundance of HDNA-Bacteria; Bacterial abundance of LDNA-Bacteria; bacterial production; BIBS; Bridging in Biodiversity Science; chlorophyll; Climate change; Climate driven Changes in Biodiversity of Microbiota; cyanobacteria; DATE/TIME; Day of experiment; DCM; DEPTH, water; derived; Description; disturbance; enclosure experiment; Enclosure experiment; Epifluorescence microscopy after DAPI staining; Flow cytometry; Free-living bacterial abundance; Germany; lake; Lake_Stechlin; mesocosm experiment; Mesocosm label; NITROLIMIT; Nostocales biovolume; Nostocales cell abundance; Oxygen/Nitrogen ratio; Particle-associated bacterial abundance; Picocyanobacteria abundance; Protein production, free-living bacteria; Protein production, particle associated bacteria; Stickstofflimitation in Binnengewässern; summer storm; TemBi; Treatment
Materialart:
Dataset
Format:
text/tab-separated-values, 2003 data points
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