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  • 1
    Electronic Resource
    Electronic Resource
    Attachment of PC12 Cells to Adhesion Substratum Induces the Accumulation of Glucose Transporters (GLUTs) and Stimulates Glucose Metabolism (1998)
    Springer
    Neurochemical research 23 (1998), S. 1107-1116 
    Details
    ISSN: 1573-6903
    Keywords: Glucose transporters ; GLUT3 ; PC12 cells ; neuronal differentiation ; poly-l-lysine
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract The levels of glucose transporters (GLUTs), specifically GLUT3 and GLUT1, increased dramatically in PC12 cells that were cultured on suitable adhesion substrata (poly-l-lysine [PLL]) and induced to differentiate with nerve growth factor (NGF). Closer examination of this response revealed that: (1) cellular attachment to PLL was sufficient to stimulate the increase in GLUT immunoreactivity, and (2) NGF alone was not effective unless the cells were cultured on PLL-treated surfaces. The response to PLL was detected as early as 4 hr after plating the cells and peaked within 24–48 hr. Other adhesion substrata, such as collagen and poly-l-ornithine, evoked a similar response, although the latter polymer was far less effective. The increase in GLUTs appeared to result from an accumulation of existing transporters because this response was not blocked by inhibiting protein synthesis. Cellular adhesion to PLL was also accompanied by a rapid activation of glucose metabolism. Thus, specific recognition of the adhesion substratum not only provides a context for cell attachment, but also elicits important functional changes in GLUT activity.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1023/A:1020768321358
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    Paper (German National Licenses)
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  • 2
    Electronic Resource
    Electronic Resource
    Cationic polymerization of 1,3-dioxepane in the presence of 2,2-bis(hydroxymethyl)butanol (1998)
    Bognor Regis [u.a.] : Wiley-Blackwell
    Journal of Polymer Science Part A: Polymer Chemistry 36 (1998), S. 2899-2903 
    Details
    ISSN: 0887-624X
    Keywords: cationic ring-opening polymerization ; poly(1,3-dioxepane) triol ; 2,2-bis(hydroxymethyl)butanol ; transacetalization ; activated monomer mechanism ; activated chain mechanism ; Chemistry ; Polymer and Materials Science
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
    Notes: Cationic polymerization of 1,3-dioxepane (DOP) initiated by triflic acid was carried out in the presence of 2,2-bis(hydroxymethyl)butanol (BHMB). The structure and molecular weight of the products were characterized by GPC and NMR spectra. The results showed that molecular weight of the polyacetal obtained could be controlled by the initial mole ratio of DOP/BHMB. GPC showed that as the mole ratio of BHMB/DOP increased, the content of cyclic oligomers also increased. Proton, 13C and 2D HMQC-fg NMR demonstrated that no hydroxymethyl group of BHMB appeared as an end group. It was also illustrated by proton NMR that some BHMB units existed in cyclic oligomers. The mechanism of formation of cyclic oligomers was discussed. © 1998 John Wiley & Sons, Inc. J Polym Sci A: Polym Chem 36: 2899-2903, 1998
    Additional Material: 4 Ill.
    Type of Medium: Electronic Resource
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