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  • 1H NMR spectroscopy; Acetate, per wet mass; Adenosine diphosphate, per wet mass; Adenosine monophosphate, per wet mass; Adenosine triphosphate, per wet mass; Alanine; Anserine; Arginine; Asparagine; Aspartic acid; Asterias_rubens_2018; Baltic Sea, Eckernförde Bay; Baltic Sea, Kattegat; BD; beta-Alanine; Betaine; Bromide; Calcium; Calculated average/mean values; Carnitine; cellular volume regulation; Chloride; Choline; Climate change; Creatine; Creatinephosphate; Creatinine; Date/time end, experiment; Date/time start, experiment; Diadumene_lineata_2018; Dimethylamine; Dimethylsulfone; Dredge, benthos; Event label; Flame photometry; Fluoride; Freeze-depression osmometer, Gonotec, Osmomat 030; Glutamic acid; Glutamine; Glycine; HAND; Histamine; Homarine 5; Homocysteine; Homoserine; Hydroxyacetone; Hypotaurine; Individual code; Inosine; invertebrates; Ion chromatography; Isoleucine; Kieler Bucht; Laboratory experiment; Lactate, per wet mass; L-Arginine; Leucine; Littorina_littorea_2018; Lysine; Malonate; Medium; Metabolite, unassigned; Methylamine; Methylmalonate; Mytilus_edulis_2018; Nitrate; N-Methylhydantoin; O-Acetylcholine; O-Phosphocholine; Ornithine; osmoconformer; Osmolality; osmolytes; osmoregulation; Phenylalanine; Phosphate; pi-Methylhistidine; Potassium; Proline; Psammechinus_miliaris_2018; salinity tolerance; Sampling by hand; Sarcosine; Serine; sn-Glycero-3-phosphocholine; Sodium; Species, unique identification; Species, unique identification (Semantic URI); Species, unique identification (URI); Strongylocentrotus_droebachiensis_2018; Succinate, per wet mass; Sulfate; Tank number; tau-Methylhistidine; Taurine; Temperature, water; Threonine; Treatment: salinity; Trimethylamine N-oxide; Tryptophan; Type of study; Tyrosine; Valine; VID; Visual identification  (1)
  • Abundance change; Alkalinity, total; Aragonite saturation state; Asterias rubens; Asterias rubens, survival; Balanus improvisus; Balanus improvisus, plate, growth rate; Benthos; Bicarbonate ion; Calcite saturation state; Calculated using seacarb after Nisumaa et al. (2010); Carbon, inorganic, dissolved; Carbonate ion; Carbonate system computation flag; Carbon dioxide; Coast and continental shelf; Community composition and diversity; Entire community; Experiment; Fucus vesiculosus; Fucus vesiculosus, length, growth rate; Fugacity of carbon dioxide (water) at sea surface temperature (wet air); Growth/Morphology; Laboratory experiment; Mesocosm or benthocosm; Mortality/Survival; Mytilus edulis; Mytilus edulis, shell length, growth rate; North Atlantic; OA-ICC; Ocean Acidification International Coordination Centre; Partial pressure of carbon dioxide (water) at sea surface temperature (wet air); pH; Replicate; Rocky-shore community; Salinity; Season; Temperate; Temperature; Temperature, water; Treatment; Type of study  (1)
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  • 1
    Publikationsdatum: 2023-11-08
    Beschreibung: Laboratory experiments were conducted in the climate chambers at GEOMAR Helmholtz Centre for Ocean Research Kiel in the time between March and November 2018. Experiments were designed to study the effect of long-term (1 month) exposure to low salinity in osmoconforming invertebrates. The study organisms (Asterias rubens, Mytilus edulis, Littorina littorea, Diadumene lineata, Strongylocentrotus droebachiensis and Psammechinus milliaris) were collected in Kiel Fjord, Eckernförder Bight or the Kattegat from spring to autumn 2018. Organisms were acclimated to climate chamber conditions for 1 week (under habitat salinity, 14˚C, constant aeration) and then subjected to salinity acclimation for 1-2 weeks until the final salinity treatment level was reached. Then different salinity treatments were maintained for 4 weeks. Water physiochemistry (temperature, salinity, pH, nitrite, nitrate, phosphate) was recorded frequently. After the experiment, samples were taken from tissues to measure total osmolality (mosmol/kg) with an osmomat, and inorganic ions (mmol/kg or µmol/g wet mass). Anions were measured with a novel protocol via ion chromatography, cations were measured via flame photometry. Organic osmolytes were measured via 1H-NMR.
    Schlagwort(e): 1H NMR spectroscopy; Acetate, per wet mass; Adenosine diphosphate, per wet mass; Adenosine monophosphate, per wet mass; Adenosine triphosphate, per wet mass; Alanine; Anserine; Arginine; Asparagine; Aspartic acid; Asterias_rubens_2018; Baltic Sea, Eckernförde Bay; Baltic Sea, Kattegat; BD; beta-Alanine; Betaine; Bromide; Calcium; Calculated average/mean values; Carnitine; cellular volume regulation; Chloride; Choline; Climate change; Creatine; Creatinephosphate; Creatinine; Date/time end, experiment; Date/time start, experiment; Diadumene_lineata_2018; Dimethylamine; Dimethylsulfone; Dredge, benthos; Event label; Flame photometry; Fluoride; Freeze-depression osmometer, Gonotec, Osmomat 030; Glutamic acid; Glutamine; Glycine; HAND; Histamine; Homarine 5; Homocysteine; Homoserine; Hydroxyacetone; Hypotaurine; Individual code; Inosine; invertebrates; Ion chromatography; Isoleucine; Kieler Bucht; Laboratory experiment; Lactate, per wet mass; L-Arginine; Leucine; Littorina_littorea_2018; Lysine; Malonate; Medium; Metabolite, unassigned; Methylamine; Methylmalonate; Mytilus_edulis_2018; Nitrate; N-Methylhydantoin; O-Acetylcholine; O-Phosphocholine; Ornithine; osmoconformer; Osmolality; osmolytes; osmoregulation; Phenylalanine; Phosphate; pi-Methylhistidine; Potassium; Proline; Psammechinus_miliaris_2018; salinity tolerance; Sampling by hand; Sarcosine; Serine; sn-Glycero-3-phosphocholine; Sodium; Species, unique identification; Species, unique identification (Semantic URI); Species, unique identification (URI); Strongylocentrotus_droebachiensis_2018; Succinate, per wet mass; Sulfate; Tank number; tau-Methylhistidine; Taurine; Temperature, water; Threonine; Treatment: salinity; Trimethylamine N-oxide; Tryptophan; Type of study; Tyrosine; Valine; VID; Visual identification
    Materialart: Dataset
    Format: text/tab-separated-values, 5452 data points
    Standort Signatur Einschränkungen Verfügbarkeit
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  • 2
    Publikationsdatum: 2024-03-15
    Beschreibung: The plea for using more “realistic,” community‐level, investigations to assess the ecological impacts of global change has recently intensified. Such experiments are typically more complex, longer, more expensive, and harder to interpret than simple organism‐level benchtop experiments. Are they worth the extra effort? Using outdoor mesocosms, we investigated the effects of ocean warming (OW) and acidification (OA), their combination (OAW), and their natural fluctuations on coastal communities of the western Baltic Sea during all four seasons. These communities are dominated by the perennial and canopy‐forming macrophyte Fucus vesiculosus—an important ecosystem engineer Baltic‐wide. We, additionally, assessed the direct response of organisms to temperature and pH in benchtop experiments, and examined how well organism‐level responses can predict community‐level responses to the dominant driver, OW. OW affected the mesocosm communities substantially stronger than acidification. OW provoked structural and functional shifts in the community that differed in strength and direction among seasons. The organism‐level response to OW matched well the community‐level response of a given species only under warm and cold thermal stress, that is, in summer and winter. In other seasons, shifts in biotic interactions masked the direct OW effects. The combination of direct OW effects and OW‐driven shifts of biotic interactions is likely to jeopardize the future of the habitat‐forming macroalga F. vesiculosus in the Baltic Sea. Furthermore, we conclude that seasonal mesocosm experiments are essential for our understanding of global change impact because they take into account the important fluctuations of abiotic and biotic pressures.
    Schlagwort(e): Abundance change; Alkalinity, total; Aragonite saturation state; Asterias rubens; Asterias rubens, survival; Balanus improvisus; Balanus improvisus, plate, growth rate; Benthos; Bicarbonate ion; Calcite saturation state; Calculated using seacarb after Nisumaa et al. (2010); Carbon, inorganic, dissolved; Carbonate ion; Carbonate system computation flag; Carbon dioxide; Coast and continental shelf; Community composition and diversity; Entire community; Experiment; Fucus vesiculosus; Fucus vesiculosus, length, growth rate; Fugacity of carbon dioxide (water) at sea surface temperature (wet air); Growth/Morphology; Laboratory experiment; Mesocosm or benthocosm; Mortality/Survival; Mytilus edulis; Mytilus edulis, shell length, growth rate; North Atlantic; OA-ICC; Ocean Acidification International Coordination Centre; Partial pressure of carbon dioxide (water) at sea surface temperature (wet air); pH; Replicate; Rocky-shore community; Salinity; Season; Temperate; Temperature; Temperature, water; Treatment; Type of study
    Materialart: Dataset
    Format: text/tab-separated-values, 1200 data points
    Standort Signatur Einschränkungen Verfügbarkeit
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