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  • Wiley-Blackwell  (2)
  • Blackwell Publishers  (1)
  • 1
    ISSN: 1469-7610
    Quelle: Blackwell Publishing Journal Backfiles 1879-2005
    Thema: Medizin , Psychologie
    Notizen: Background: This study focuses on a novel observational paradigm (SNAP) involving a rigged competitive card game (Murray, Woolgar, Cooper, & Hipwell, 2001) designed to expose children to the threat of losing. Recent work suggests that this paradigm is useful for assessing disruptive behaviour in young children (Hughes, Cutting, & Dunn, 2001). Method: We report on a large study (involving 800 five-year-olds) that compares observational ratings of disruptive behaviour on the SNAP game with mother and teacher reports of externalising behaviour on the CBCL and TRF (Achenbach, 1991a, 1991b). To ensure independence of data, playmates were randomly assigned to two different sub-samples. The validity of this rigged game for examining individual differences in disruptive behaviour was supported (in both sub-samples) by modest but significant correlations with both mother and teacher ratings of externalising problems, and by significantly elevated SNAP ratings among children rated by mothers and teachers as showing extreme (≥95th %) levels of externalising problems, compared with the remaining majority of children. Results: Significant gender differences in disruptive behaviour were found on all three measures: observational SNAP ratings and mother/teacher questionnaire ratings. Factors that may contribute to this gender difference are discussed. Conclusions: Our findings emphasise the importance of multi-method, multi-informant measures of disruptive behaviour, and suggest that the rigged card game used in this study is a valuable adjunct to more standard methods of rating disruptive behaviour.
    Materialart: Digitale Medien
    Standort Signatur Einschränkungen Verfügbarkeit
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  • 2
    Publikationsdatum: 2016-03-18
    Beschreibung: ABSTRACT Tumour Necrosis Factor- Alpha (TNF-α) is chronically elevated in conditions where skeletal muscle loss occurs. As L-glutamine can dampen the effects of inflamed environments, we investigated the role of L-glutamine in both differentiating C2C12 myoblasts and existing myotubes in the absence/presence of TNF-α (20 ng.ml −1 ) ± L-glutamine (20 mM).TNF-α reduced the proportion of cells in G1 phase, as well as biochemical (CK activity) and morphological differentiation (myotube number), with corresponding reductions in transcript expression of: Myogenin, Igf-I and Igfbp5 . Furthermore, when administered to mature myotubes, TNF-α induced myotube loss and atrophy underpinned by reductions in Myogenin, Igf-I, Igfbp2 and glutamine synthetase and parallel increases in Fox03 , Cfos, p53 and Bid gene expression. Investigation of signaling activity suggested that Akt and ERK1/2 were unchanged, JNK increased (non-significantly) whereas P38 MAPK substantially and significantly increased in both myoblasts and myotubes in the presence of TNF-α. Importantly, 20 mM L-glutamine reduced p38 MAPK activity in TNF-α conditions back to control levels, with a corresponding rescue of myoblast differentiation and a reversal of atrophy in myotubes. L-glutamine resulted in upregulation of genes associated with growth and survival including; Myogenin, Igf-Ir, Myhc2 & 7, Tnfsfr1b, Adra1d and restored atrophic gene expression of Fox03 back to baseline in TNF-α conditions. In conclusion, L-glutamine supplementation rescued suppressed muscle cell differentiation and prevented myotube atrophy in an inflamed environment via regulation of p38 MAPK. L-glutamine administration could represent an important therapeutic strategy for reducing muscle loss in catabolic diseases and inflamed ageing. This article is protected by copyright. All rights reserved
    Digitale ISSN: 1097-4652
    Thema: Biologie , Medizin
    Publiziert von Wiley-Blackwell
    Standort Signatur Einschränkungen Verfügbarkeit
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  • 3
    Publikationsdatum: 2016-07-30
    Beschreibung: Aims Recent literature suggests that clinically silent, microscopic GISTs are common incidental findings. The aim of this study was to examine the histological, immunohistochemical and molecular characteristics of these tumours, which we have defined as measuring ≤ 20 mm, in order to determine if the rate and spectrum of mutations is similar to that of clinically symptomatic GISTs Methods and results 13 microscopic GISTs identified as incidental findings in patients undergoing management of concomitant disease were tested for KIT and PDGFRA mutations. 10 micro-GISTs (77%) were located in the stomach, two (15%) in the duodenum and one (8%) in the rectum. The mean tumour size was 9.3 mm (range2 – 19 mm). All tumours were well-circumscribed lesions showing a predominantly spindle cell morphology and a very low mitotic rate. 12 out of 13 (92%) tumours carried mutations in either KIT (83%) or PDGFRA (17%), a rate higher than in other published series. A high mutation rate (80%) was also seen in lesions ≤ 5mm. Conclusions Our results suggest that KIT / PDGFRA mutation is a very common early event in GIST development, that tumour size does not reliably predict the presence of mutation and that one or more subsequent mutations are required for clinical manifestation. This article is protected by copyright. All rights reserved.
    Print ISSN: 0309-0167
    Digitale ISSN: 1365-2559
    Thema: Medizin
    Publiziert von Wiley-Blackwell
    Standort Signatur Einschränkungen Verfügbarkeit
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