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  • 1
    ISSN: 1423-0127
    Keywords: Apoptosis ; Differential display ; Glioma ; Okadaic acid
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Abstract To identify novel genes associated with apoptosis in glioma cells, we treated T98G glioma cells with okadaic acid (OA). Differential display using 15 random primers was performed on RNA extracted from these cells. Upregulated bands were excised from polyacrylamide gels and cloned. Northern blots were used to confirm RNA expression in T98G cells. 18 RNA fragments corresponding to the untranslated region of genes were identified and sequenced. Three unknown gene fragments were used to screen a fetal brain cDNA library resulting in three complete cDNA sequences. The three sequences corresponded to a human gene homologous to the yeast translation initiation factor Sui-1, a cAMP-regulated phosphoprotein, ARPP-16/19, and a novel gene designated O48. Transcription of Sui-1 increased in response to all stress factors tested, whereas ARPP only responded to OA. 2-kb and 4-kb O48 RNA species were identified. OA and stress factors increased 2-kb expression while K252a (protein kinase inhibitor) increased 4-kb expression. Differential display is effective for identifying genes associated with apoptosis. Novel genes may be identified by further analysis of the gene fragments identified in this study. The function of O48 is unknown.
    Type of Medium: Electronic Resource
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  • 2
    ISSN: 1432-2013
    Keywords: Tyrosine kinase inhibitor hKv1.4 Genistein Vanadate Potassium channels Herbimycin A
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract. A cloned, human, A-type, voltage-gated potassium channel (hKv1.4) was expressed transiently in Chinese hamster ovary cells and the effects of the broad-spectrum tyrosine kinase inhibitor genistein on hKv1.4 were studied using the whole-cell patch-clamp recording method. Genistein (up to 50 µM) reversibly reduced the peak currents of hKv1.4 by 44.9±12%. In addition, genistein markedly slowed the activation kinetics (time constant τa) of hKv1.4. At +50 mV, τa increased from 1.8±0.3 to 5.0±0.6 ms (P〈0.01). The effect of genistein on the channel inactivation kinetics (time constant τi) was more complex, in that τi was increased significantly at lower step potentials but unaltered at +50 mV or more depolarized potentials. Tail current analysis showed that genistein had no effect on the kinetics of deactivation (time constant τd), but shifted the steady-state activation curve significantly to the right by about 15 mV (potential for half-maximal activation, V 1/2, changed from –7.4±4.4 to +7.7±2.7 mV) with a moderate change in the slope (k) of the curve (from 17.4±2.2 to 23±1.0 mV, P〈0.05). Genistein slightly altered the slope of the steady-state inactivation curve from –5.5±0.4 to –7.5±0.4 mV (P〈0.01). The recovery rate from inactivation was not altered by genistein. The tyrosine phosphatase inhibitor orthovanadate (1 mM) alone had little impact on current amplitude or channel kinetics. However, orthovanadate significantly, but not completely, blocked the effect of genistein on current amplitude (by 25.5%) and kinetics (by 67.1%). Daidzein (up to 50 µM), an inactive analogue of genistein, had no effect on current amplitude or kinetics. In contrast to genistein, another tyrosine kinase inhibitor, herbimycin A, had little effect on the channel peak amplitude or kinetics. In addition, genistein had a similar impact on the channel peak current amplitude and kinetics in cells with or without pre-treatment with herbimycin A (10 µM). The data suggest that genistein-induced inhibition of tyrosine phosphorylation may not be the exclusive mechanism by which hKv1.4 is down-regulated and channel gating affected. Genistein may produce a non-catalytic blockade of this channel.
    Type of Medium: Electronic Resource
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  • 3
    Electronic Resource
    Electronic Resource
    Springer
    International journal of infrared and millimeter waves 17 (1996), S. 205-215 
    ISSN: 1572-9559
    Keywords: millimeter wave ; H-waveguide ; sensor ; measurement for humidity
    Source: Springer Online Journal Archives 1860-2000
    Topics: Physics
    Notes: Abstract In this paper, we present a new kind of millimeter wave sensor which is constituted by double-strip H-wave-guide. This sensor has some major advantages such as big size, simple structure and good measurement performance. It is particularly suitable for measuring humidities of pellet materials at microwave band.
    Type of Medium: Electronic Resource
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  • 4
    ISSN: 1572-9559
    Keywords: MSL ; monolithic microwave integrated circuits (MMICs) ; quasi-static characteristics ; metallization thickness ; FDM
    Source: Springer Online Journal Archives 1860-2000
    Topics: Physics
    Notes: Abstract A shielded microstripline (MSL) of elliptic cross-section with finite metallization thickness penetrating into the substrate is presented. The quasi-static characteristics of this kind of MSL are studied with finit difference method (FDM). The effect of metal cross-section shape and metal penetrating depth is also studied.
    Type of Medium: Electronic Resource
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  • 5
    Electronic Resource
    Electronic Resource
    Springer
    International journal of infrared and millimeter waves 17 (1996), S. 2195-2206 
    ISSN: 1572-9559
    Keywords: elliptic-groove waveguides ; dispersion characteristics ; Mathieu function ; mode-matching method
    Source: Springer Online Journal Archives 1860-2000
    Topics: Physics
    Notes: Abstract In this paper, two kinds of elliptic-groove waveguides are presented. One of them has never been studied before. With the help of Mathieu function and mode-matching method, the field components and characteristic equation of the elliptic-groove waveguides are obtained for the first time. Then their dispersion characteristics for the dominant mode i.e. the mode TE e11 1 are also studied. The gotten conclusions have very important values in theoretical study and actual application for the two kinds of the elliptic-groove waveguides.
    Type of Medium: Electronic Resource
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  • 6
    ISSN: 1436-2813
    Keywords: colorectal cancer ; transitional mucosa ; histochemistry ; mucins ; tumor recurrence
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract The underlying nature of the transitional mucosa adjacent to colorectal cancer is defined and the evidence for and against the statement that this transitional mucosa involves primary premalignant change presented in this article. An association between mucin histochemical changes at the margins of resection and a poorer clinical outcome of patients has been recognized in patients with colorectal cancer after surgery. The retained transitional mucosa at the margins of resection appears to correlate with tumor recurrence and a poorer survival in patients who have undergone radical resection. It is considered that the transitional mucosa adjacent to colorectal cancer and its presence at the margins of resection may be an important prognostic marker for patients with large bowel cancer following radical resection.
    Type of Medium: Electronic Resource
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  • 7
    ISSN: 1530-0358
    Keywords: Colorectal carcinomas ; Transitional mucosa ; DNA content ; Sialomucin ; Image cytometry
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract The transitional mucosa (TM) adjacent to carcinomas of the large bowel shows histologic and mucin histochemical changes that may indicate premalignant change and may be of prognostic value after radical resection. In this study, 10 anterior resection specimens from patients with carcinomas of the rectum and rectosigmoid were used to compare the nuclear DNA content in TM with those in cancer tissue and with those in nontransitional mucosa (N-TM; i.e.,uninvolved mucosa remote from tumors showing normal histologic and mucin histochemical features). The nuclear DNA content was assessed using DNA image cytometry on Feulgen-stained sections. As compared with N-TM, crypts in TM contained greater numbers of cells, were elongated, and were more likely to be branched with marked sialomucin secretion, accompanied by a marked reduction in the normal sulfomucin content. The mean nuclear DNA content in the upper, middle, and lower thirds of crypts was significantly higher in TM than in N-TM, and the nuclear DNA content in TM exhibited no correlation to that in tumors. The results suggest that TM adjacent to carcinomas of the rectum and rectosigmoid has higher proliferative activity, providing further evidence that TM may be an unstable premalignant change.
    Type of Medium: Electronic Resource
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  • 8
    Electronic Resource
    Electronic Resource
    Springer
    Journal of statistical physics 78 (1995), S. 799-813 
    ISSN: 1572-9613
    Keywords: Correlation exponent ; correlation dimension ; Hill estimator ; Monte Carlo method ; bootstrap
    Source: Springer Online Journal Archives 1860-2000
    Topics: Physics
    Notes: Abstract We propose a Monte Carlo method for estimating the correlation exponent of a stationary ergodic sequence. The estimator can be considered as a bootstrap version of the classical Hill estimator. A simulation study shows that the method yields reasonable estimates.
    Type of Medium: Electronic Resource
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  • 9
    Publication Date: 2017-01-27
    Description: A new climate model has been developed that employs a multi-resolution dynamical core for the sea ice-ocean component. In principle, the multi-resolution approach allows one to use enhanced horizontal resolution in dynamically active regions while keeping a coarse-resolution setup otherwise. The coupled model consists of the atmospheric model ECHAM6 and the finite element sea ice-ocean model (FESOM). In this study only moderate refinement of the unstructured ocean grid is applied and the resolution varies from about 25 km in the northern North Atlantic and in the tropics to about 150 km in parts of the open ocean; the results serve as a benchmark upon which future versions that exploit the potential of variable resolution can be built. Details of the formulation of the model are given and its performance in simulating observed aspects of the mean climate is described. Overall, it is found that ECHAM6–FESOM realistically simulates many aspects of the observed climate. More specifically it is found that ECHAM6–FESOM performs at least as well as some of the most sophisticated climate models participating in the fifth phase of the Coupled Model Intercomparison Project. ECHAM6–FESOM shares substantial shortcomings with other climate models when it comes to simulating the North Atlantic circulation.
    Repository Name: EPIC Alfred Wegener Institut
    Type: Article , isiRev
    Format: application/pdf
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  • 10
    Publication Date: 2022-07-13
    Description: Growing evidence indicates that the atmospheric and oceanic circulation experiences a systematic poleward shift in a warming climate. However, the complexity of the climate system, including the coupling between the ocean and the atmosphere, natural climate variability and land-sea distribution, tends to obfuscate the causal mechanism underlying the circulation shift. Here, using an idealised coupled aqua-planet model, we explore the mechanism of the shifting circulation, by isolating the contributing factors from the direct CO2 forcing, the indirect ocean surface warming, and the wind-stress feedback from the ocean dynamics. We find that, in contrast to the direct CO2 forcing, ocean surface warming, in particular an enhanced subtropical ocean warming, plays an important role in driving the circulation shift. This enhanced subtropical ocean warming emerges from the background Ekman convergence of surface anomalous heat in the absence of the ocean dynamical change. It expands the tropical warm water zone, causes a poleward shift of the mid-latitude temperature gradient, hence forces a corresponding shift in the atmospheric circulation and the associated wind pattern. The shift in wind, in turn drives a shift in the ocean circulation. Our simulations, despite being idealised, capture the main features of the observed climate changes, for example, the enhanced subtropical ocean warming, poleward shift of the patterns of near-surface wind, sea level pressure, storm tracks, precipitation and large-scale ocean circulation, implying that increase in greenhouse gas concentrations not only raises the temperature, but can also systematically shift the climate zones poleward.
    Repository Name: EPIC Alfred Wegener Institut
    Type: Article , NonPeerReviewed
    Format: application/pdf
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