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  • Blackwell Publishing Ltd  (2)
  • IOP Science  (1)
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  • 1
    Electronic Resource
    Electronic Resource
    Oxford, UK : Blackwell Publishing Ltd
    FEMS immunology and medical microbiology 17 (1997), S. 0 
    ISSN: 1574-695X
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology , Medicine
    Notes: Arbitrary primed PCR (AP-PCR) analysis was compared with serotyping as a means of high-resolution typing of Pseudomonas aeruginosa. Seventy-four isolates from 3 different hospitals and 18 reference strains were studied. Serotyping provided good index of discrimination, although eleven isolates could not be serotyped. Genomic DNA was amplified with a single 10 nucleotide primer (sequence 5′-AGG GGT CTT G-3′). The strains were genetically diverse and 61 different AP-PCR profiles of 2–7 bands between 0.3 and 2.4 kb were obtained. AP-PCR profiles were not consistently associated with serotypes, but they clearly subtyped strains of the same serotype. Numerical analysis of AP-PCR patterns defined 7 groups at the 55% similarity level, and identified predominant strains in each hospital. The results show that AP-PCR analysis provides a simple and practical approach to typing P. aeruginosa that is more discriminatory than traditional serotyping scheme. We suggest that maximum discrimination can be achieved by a combination of both methods.
    Type of Medium: Electronic Resource
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  • 2
    ISSN: 1574-6968
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Notes: In this study, we evaluated the combination of polymerase chain reaction–restriction fragment length polymorphism (PCR–RFLP) and amplified fragment length polymorphism (AFLP) molecular typing techniques for the analysis of thermophilic campylobacter species isolated from clinical and poultry samples. 23S PCR–RFLP analysis performed to fingerprint 69 strains exhibited an excellent level of typability. Eleven different types were defined at 100% linkage level following numerical analysis of band patterns. Differentiation of Campylobacter jejuni and Campylobacter coli at species level was achieved although no significant relationship could be observed between the profiles and the origin of the strains. Simplified AFLP analysis of the isolates disclosed the presence of 66 different banding patterns. The resulting dendrogram showed a high diversity among the strains studied. All the isolates were grouped within eight main types with a 69% homology degree among them. Differentiation at subspecies level was possible but no significant relationship could be observed between the AFLP profiles and the origin of the strains. When used in combination, 23S PCR–RFLP and single-enzyme AFLP methods can be applied to determine taxonomic and epidemiological relationships among thermophilic campylobacters.
    Type of Medium: Electronic Resource
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  • 3
    Publication Date: 2022-05-25
    Description: © The Author(s), 2015. This article is distributed under the terms of the Creative Commons Attribution License. The definitive version was published in Environmental Research Letters 10 (2015): 074010, doi:10.1088/1748-9326/10/7/074010.
    Description: Spatially extensive and persistent drought episodes have repeatedly influenced human history, including the 'Strange Parallels' drought event in monsoon Asia during the mid-18th century. Here we explore the dynamics of sustained monsoon failure using observed and tree-ring reconstructed drought patterns and a 1300-year pre-industrial community earth system model control run. Both modern observational and climate model drought patterns during years with extremely weakened South Asian monsoon resemble those reconstructed for the Strange Parallels drought. Model analysis reveals that this pattern arises during boreal spring over Southeast Asia, with decreased precipitation and moisture flux, while related summertime climate anomalies are confined to the Indian subcontinent. Years with simulated South Asian drying exhibit canonical El Niño conditions over the Pacific and associated shifts in the Walker circulation. In contrast, multi-year drought periods, resembling those sustained during the Strange Parallels drought, feature anomalous Pacific warming around the dateline, typical of El Niño Modoki events.
    Description: This work was performed with support and funding from the Significant Opportunities in Atmospheric Research and Sciences Program (NSF AGS-1120459), WHOI Academic Programs Office Funds, and NSF AGS-1338734, AGS-1203704, and AGS-1304245.
    Repository Name: Woods Hole Open Access Server
    Type: Article
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