Description: The last deglaciation was characterized by a sequence of abrupt climate events thought to be linked to rapid changes in Atlantic meridional overturning circulation (AMOC). The sequence includes a weakening of the AMOC after the Last Glacial Maximum (LGM) during Heinrich Stadial 1 (HS1), which ends with an abrupt AMOC amplification at the transition to the Bølling/Allerød (B/A). This transition occurs despite persistent deglacial meltwater fluxes that counteract vigorous North Atlantic deep-water formation. Using the Earth system model COSMOS with a range of deglacial boundary conditions and reconstructed deglacial meltwater fluxes, we show that deglacial CO2 rise and ice sheet decline modulate the sensitivity of the AMOC to these fluxes. While declining ice sheets increase the sensitivity, increasing atmospheric CO2 levels tend to counteract this effect. Therefore, the occurrence of a weaker HS1 AMOC and an abrupt AMOC increase in the presence of meltwater, might be explained by these effects, as an alternative to or in combination with changes in the magnitude or routing of meltwater discharge.

Description: The cause of rapid hydrological changes in the tropical West Pacific during the last deglaciation remains controversial. In order to test whether these changes were triggered by abrupt climate change events in the North Atlantic Ocean, variations in precipitation during the last deglaciation (18–10 ka) were extracted from proxy records of chemical weathering and terrigenous input in the western Philippine Sea (WPS). The evolution of chemical weathering and terrigenous input since 27 ka was reconstructed using the chemical index of alteration (CIA), elemental ratios (K/Al, TOC/TN and Ti/Ca), δ13Corg, terrigenous fraction abundance and flux data from International Marine Global Change Study Program (IMAGES) core MD06-3054 collected on the upper continental slope of eastern Luzon (northern Philippines). Sediment deposited during the Last Glacial Maximum (LGM) shows weathering equal to or slightly greater than Holocene sediment in the WPS. This unusual state of chemical weathering, which is inconsistent with lower air temperatures and decreased precipitation in Luzon during the LGM, may be due to reworking of poorly consolidated sediments on the eastern Luzon continental shelf during the LGM sea-level lowstand. Rapid changes in chemical weathering, characterized by higher intensity during the Heinrich event 1 (H1) and Younger Dryas (YD) and lower intensity during the Bølling-Allerød (B/A), were linked to rapid variations in precipitation in the WPS during the last deglaciation. The higher terrigenous inputs during the LGM relative to those of the Holocene were controlled by sea-level changes rather than precipitation. The terrigenous inputs show a long-term decline during the last deglaciation, punctuated by brief spikes during the H1 and YD related to sea-level rises and rapid precipitation changes in the WPS, respectively. The proxy records of chemical weathering and terrigenous input from eastern Luzon suggest high rainfall during the H1 and YD events, consistent with inferred rainfall patterns based on Fe/Ca records from offshore Mindanao. Rapid precipitation changes in the WPS did not coincide with migrations of the Intertropical Convergence Zone (ITCZ) but, rather, were related to state shifts of the El Niño-Southern Oscillation (ENSO) during the last deglaciation. Based on proxy records and modeling results, we argue that the Atlantic meridional overturning circulation (AMOC) controlled rapid precipitation changes in the tropical West Pacific through zonal shifts of ENSO or meridional migration of the ITCZ during the last deglaciation. Our findings highlight the dominant role of the North Atlantic Ocean in the tropical hydrologic cycle during the last deglaciation.

Description: Climate models are potentially useful tools for addressing human dispersals and demographic change. The Arabian Peninsula is becoming increasingly significant in the story of human dispersals out of Africa during the Late Pleistocene. Although characterised largely by arid environments today, emerging climate records indicate that the peninsula was wetter many times in the past, suggesting that the region may have been inhabited considerably more than hitherto thought. Explaining the origins and spatial distribution of increased rainfall is challenging because palaeoenvironmental research in the region is in an early developmental stage. We address environmental oscillations by assembling and analysing an ensemble of five global climate models (CCSM3, COSMOS, HadCM3, KCM, and NorESM). We focus on precipitation, as the variable is key for the development of lakes, rivers and savannas. The climate models generated here were compared with published palaeoenvironmental data such as palaeolakes, speleothems and alluvial fan records as a means of validation. All five models showed, to varying degrees, that the Arabia Peninsula was significantly wetter than today during the Last Interglacial (130 ka and 126/125 ka timeslices), and that the main source of increased rainfall was from the North African summer monsoon rather than the Indian Ocean monsoon or from Mediterranean climate patterns. Where available, 104 ka (MIS 5c), 56 ka (early MIS 3) and 21 ka (LGM) timeslices showed rainfall was present but not as extensive as during the Last Interglacial. The results favour the hypothesis that humans potentially moved out of Africa and into Arabia on multiple occasions during pluvial phases of the Late Pleistocene.

Description: Background: High-resolution cytogenetic map can provide not only important biological information on genome organization but also solid foundation for genetic and genomic research. The progress in the molecular and cytogenetic studies has created the basis for developing the cytogenetic map in cucumber (Cucumis sativus L.). Results: Here, the cytogenetic maps of four cucumber chromosomes (chromosomes 1, 3--5) were constructed by fluorescence in situ hybridization (FISH) analysis on cucumber pachytene chromosomes. Together with our previously constructed cytogenetic maps of three cucumber chromosomes (chromosomes 2, 6--7), cucumber has a complete cytogenetic map with 76 anchoring points between the genetic, the cytogenetic and the draft genome assembly maps. To compare our pachytene FISH map directly to the genetic linkage and draft genome assembly maps, we used a standardized map unit---relative map position (RMP) to produce the comparative map alignments. The alignments allowed a global view of the relationship of genetic and physical distances along each cucumber chromosome, and accuracy and coverage of the draft genome assembly map. Conclusions: We demonstrated a good correlation between positions of the markers in the linkage and physical maps, and essentially complete coverage of chromosome arms by the draft genome assembly. Our study not only provides essential information for the improvement of sequence assembly but also offers molecular tools for cucumber genomics research, comparative genomics and evolutionary study.

Description: Highlights: • Climate model sensitivity experiments are performed using state-of-the-art ice sheet and freshwater reconstructions • Declining Northern Hemisphere ice sheets increase the sensitivity of the AMOC to North Atlantic meltwater discharge • Deglacial rise in atmospheric CO2 concentration decreases the sensitivity of the AMOC to North Atlantic meltwater discharge • Both effects provide a complementary perspective to existing explanations for abrupt AMOC transitions Abstract: The last deglaciation was characterized by a sequence of abrupt climate events thought to be linked to rapid changes in Atlantic meridional overturning circulation (AMOC). The sequence includes a weakening of the AMOC after the Last Glacial Maximum (LGM) during Heinrich Stadial 1 (HS1), which ends with an abrupt AMOC amplification at the transition to the Bølling/Allerød (B/A). This transition occurs despite persistent deglacial meltwater fluxes that counteract vigorous North Atlantic deep-water formation. Using the Earth system model COSMOS with a range of deglacial boundary conditions and reconstructed deglacial meltwater fluxes, we show that deglacial CO2 rise and ice sheet decline modulate the sensitivity of the AMOC to these fluxes. While declining ice sheets increase the sensitivity, increasing atmospheric CO2 levels tend to counteract this effect. Therefore, the occurrence of a weaker HS1 AMOC and an abrupt AMOC increase in the presence of meltwater, might be explained by these effects, as an alternative to or in combination with changes in the magnitude or routing of meltwater discharge.

Description: Acute diarrhea is a leading cause of morbidity and mortality in children, particularly in those under the age of 5 years. Rotavirus is recognized as the leading cause of acute diarrhea in children, however, the c...

Description: Activated hepatic stellate cell (HSC) is the main fibrogenic cell type in the injured liver. miRNA plays an important role in activation and proliferation of HSC.

Description: Background: Faba bean (Vicia faba L.) is an important food legume crop, grown for human consumption globally including in China, Turkey, Egypt and Ethiopia. Although genetic gain has been made through conventional selection and breeding efforts, this could be substantially improved through the application of molecular methods. For this, a set of reliable molecular markers representative of the entire genome is required. Results: A library with 125,559 putative SSR sequences was constructed and characterized for repeat type and length from a mixed genome of 247 spring and winter sown faba bean genotypes using 454 sequencing. A suit of 28,503 primer pair sequences were designed and 150 were randomly selected for validation. Of these, 94 produced reproducible amplicons that were polymorphic among 32 faba bean genotypes selected from diverse geographical locations. The number of alleles per locus ranged from 2 to 8, the expected heterozygocities ranged from 0.0000 to 1.0000, and the observed heterozygosities ranged from 0.0908 to 0.8410. The validation by UPGMA cluster analysis of 32 genotypes based on Nei's genetic distance, showed high quality and effectiveness of those novel SSR markers developed via next generation sequencing technology. Conclusions: Large scale SSR marker development was successfully achieved using next generation sequencing of the V. faba genome. These novel markers are valuable for constructing genetic linkage maps, future QTL mapping, and marker-assisted trait selection in faba bean breeding efforts.

Description: Background: CO and FT orthologs, belonging to the BBX and PEBP family, respectively, have important and conserved roles in the photoperiod regulation of flowering time in plants. Soybean genome experienced at least three rounds of whole genome duplications (WGDs), which resulted in multiple copies of about 75% of genes. Subsequent subfunctionalization is the main fate for paralogous gene pairs during the evolutionary process. Results: The phylogenic relationships revealed that CO orthologs were widespread in the plant kingdom while FT orthologs were present only in angiosperms. Twenty-eight CO homologous genes and twenty-four FT homologous genes were gained in the soybean genome. Based on the collinear relationship, the soybean ancestral CO ortholog experienced three WGD events, but only two paralogous gene pairs (GmCOL1/2 and GmCOL5/13) survived in the modern soybean. The paralogous gene pairs, GmCOL1/2 or GmCOL5/13, showed similar expression patterns in pair but different between pairs, indicating that they functionally diverged. GmFTL1 to 7 were derived from the same ancestor prior to the whole genome triplication (WGT) event, and after the Legume WGD event the ancestor diverged into two branches, GmFTL3/5/7 and GmFTL1/2/4/6. GmFTL7 were truncated in the N-terminus compared to other FT-lineage genes, but ubiquitously expressed. Expressions of GmFTL1 to 6 were higher in leaves at the flowering stage than that at the seedling stage. GmFTL3 was expressed at the highest level in all tissues except roots at the seedling stage, and its circadian pattern was different from the other five ones. The transcript of GmFTL6 was highly accumulated in seedling roots. The circadian rhythms of GmCOL5/13 and GmFT1/2/4/5/6 were synchronized in a day, demonstrating the complicate relationship of CO-FT regulons in soybean leaves. Over-expression of GmCOL2 did not rescue the flowering phenotype of the Arabidopsis co mutant. However, ectopic expression of GmCOL5 did rescue the co mutant phenotype. All GmFTL1 to 6 showed flower-promoting activities in Arabidopsis. Conclusions: After three recent rounds of whole genome duplications in the soybean, the paralogous genes of CO-FT regulons showed subfunctionalization through expression divergence. Then, only GmCOL5/13 kept flowering-promoting activities, while GmFTL1 to 6 contributed to flowering control. Additionally, GmCOL5/13 and GmFT1/2/3/4/5/6 showed similar circadian expression profiles. Therefore, our results suggested that GmCOL5/13 and GmFT1/2/3/4/5/6 formed the complicate CO-FT regulons in the photoperiod regulation of flowering time in soybean.

Description: Background: Staphylococcus aureus is the major cause of hospital-acquired and community-acquired pneumonia. Host defense to S.aureus infection is largely mediated by the innate immune system. gammadelta T cells play an important role in innate immunity to many infectious diseases. However, less is known about the role of these cells during S.aureus-induced pneumonia. In this study, we examined the response and the role of gammadelta T cells to pulmonary S.aureusinfection. Results: Mice infected with S. aureus intranasally showed rapid gammadelta T cells accumulation in the lung. Deficiency of gammadelta T cells led to attenuated bacterial clearance and less tissue damage in lung compared with WT mice. Moreover, TCR-delta/mice exhibited impaired neutrophilrecruitment and reduced cytokine production at the site of infection. The gammadelta T cells in response to pulmonary S. aureus infection mainly secreted IL-17 and gammadelta T cells deficiency reduced IL-17 production, which might regulate the production of neutrophil-inducingcytokine/chemokine in the S. aureus-infected lungs Conclusions: Accumulation of gammadelta T cells in the lungs to S. aureus infection is beneficial for bacteria clearance and also contributes to the tissue damage. These cells were the primary source of IL-17, which might influence the recruitment of neutrophils at the early stage of infection.