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  • 1
    Electronic Resource
    Electronic Resource
    Oxford, UK : Blackwell Publishing Ltd
    Journal of cutaneous pathology 21 (1994), S. 0 
    ISSN: 1600-0560
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Medicine
    Notes: Normal skin was cryoprotected by submerging it in a mixture of 30% dimethylformamide (DMF) in PBS or RPMI. Subsequently it was frozen in liquid propane gas. Cryosubstitution was carried out at −90°C by using methanol to which uranyl acetate or osmium tetroxide were added. The tissue was embedded in either Lowicryl K4M at −40°C or in Epon at +60°C. The- tissue was evaluated by its overall preservation of ultras-tructural details and by its labeling intensity alter incubation with either anti-clesmoglein or anti-type VII collagen monoclonal antibodies. The mixture of DMF and PBS caused an electron-dense precipitate within the cell. The overall morphology was better in Epon-embedded material than in K4M-embedclecl material. However, the labeling was best in K4M material. Regardless of whether the tissue was embedded in Epon or K4M, the addition of osmium tetroxide markedly reduced the degree of labeling.
    Type of Medium: Electronic Resource
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  • 2
    Electronic Resource
    Electronic Resource
    Oxford, UK : Blackwell Publishing Ltd
    Journal of cutaneous pathology 21 (1994), S. 0 
    ISSN: 1600-0560
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Medicine
    Notes: An-11-day old girl was seen with brownish nodular lesions scattered over (he body with emphasis on the face and seal). Several lesions had started lo involute. Tissue was studied by histopathology, immunohistopathology, routine electron microscopy, and immuno-electron microscopy using cryosubstitution and embedding in K4M. Immunohistopathology revealed that the cells of the dermal infiltrate were Langerhans cells. They expressed Leu 6 and HLA-DR. On routine electron microscopy no Birbeck granules were (bund in the dermal cells. Birbeck granules in epidermal Langerhans cells were deformed and often situated next to laminated dense bodies. The latter expressed Leu 6 and HLA-DR. on immuno-electron microscopy. It was concluded that congenital self-healing reticulohistiocytosis is a benign Langerhans cell disease in which Birbeck granules are transformed to laminated dense bodies and possibly degraded by lysosomal enzymes.
    Type of Medium: Electronic Resource
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  • 3
    Electronic Resource
    Electronic Resource
    Oxford, UK : Blackwell Publishing Ltd
    Journal of fish biology 38 (1991), S. 0 
    ISSN: 1095-8649
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Notes: In 1985 and 1986 ichthyoplankton composition was studied during seven sampling series at 37 stations along the tidal Elbe and its tributary rivers between Hamburg harbour and the North Sea. The highest larval density was observed in May and April. No, or very few, larvae occurred from late summer to early spring. Smelt (59% on average of all samples) and twaite shad (20%) dominate in the freshwater region. Herring/sprat (17%) dominate in the estuary. All major spawning grounds are located along the southern shore. Smelt spawn further upstream 1 month earlier than twaite shad. Three percent of all larvae found were flounder. Most of them occurred upstream from the estuary in the R. Este and in other tributary rivers. It is not clear whether flounders are able to spawn in freshwater. Attention is drawn to the fact that an unknown but probably very high percentage of the ichthyoplankton is entrained by local power plants.
    Type of Medium: Electronic Resource
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  • 4
    Electronic Resource
    Electronic Resource
    Oxford, UK : Blackwell Publishing Ltd
    Journal of applied ichthyology 7 (1991), S. 0 
    ISSN: 1439-0426
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology , Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: For three selected weeks in July, August, and October 1985, catches from anchor net vessels at ten stations along the tidal Elbe River (northern Germany) were analyzed and related to oxygen conditions in the river. The oxygen concentrations decreased from an average of 6.6 ml/L in the estuary to an average of 1.4 mg/L upstream. Fish were found to be concentrated downstream from the low-oxygen zone and at the entrance to a shallow side arm of the main stream. Highest catches of eel were made where oxygen levels were in the range of 1.2–3 mg/L, highest catches of flounder at 3–4 mg/L, and highest catches of juvenile and adult smelt in areas with more than 5 mgO2/L. Such a situation enables fishermen to make extraordinarily high catches. It is concluded that despite high levels of toxicants, the tidal Elbe still offers living conditions adequate for the mass of fish species as long as there is a sufficient supply of oxygen.
    Type of Medium: Electronic Resource
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  • 5
    Electronic Resource
    Electronic Resource
    Oxford [u.a.] : International Union of Crystallography (IUCr)
    Acta crystallographica 49 (1993), S. 944-946 
    ISSN: 1600-5759
    Source: Crystallography Journals Online : IUCR Backfile Archive 1948-2001
    Topics: Chemistry and Pharmacology , Geosciences , Physics
    Type of Medium: Electronic Resource
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  • 6
    Electronic Resource
    Electronic Resource
    Springer
    Journal of molecular medicine 69 (1991), S. 413-415 
    ISSN: 1432-1440
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Type of Medium: Electronic Resource
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  • 7
    Electronic Resource
    Electronic Resource
    Springer
    Naturwissenschaften 29 (1941), S. 166-168 
    ISSN: 1432-1904
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology , Natural Sciences in General
    Type of Medium: Electronic Resource
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  • 8
    Electronic Resource
    Electronic Resource
    Springer
    Archives of dermatological research 284 (1992), S. 242-245 
    ISSN: 1432-069X
    Keywords: Immunogold ; Electron microscopy ; Human skin ; Actin
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary Normal human skin was embedded in Lowicryl K4M. Actin microfilaments were localized by applyinga postembedding immunogold technique using the monoclonal anti-actin antibody HHF35. Actin microfilaments are part of the cytoskeleton in muscle and nonmuscle cells. Together with myosin they produce contraction. The antibody labelled myofilaments in smooth muscle arrector pili cells, myoepithelial cells and pericytes. In sweat gland cells the microvilli system, a zone beneath the cytoplasma membrane correponding to the adhesion belt region, and apocrine decapitation formations showed labelling.
    Type of Medium: Electronic Resource
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  • 9
    Electronic Resource
    Electronic Resource
    Springer
    Applicable algebra in engineering, communication and computing 4 (1993), S. 103-145 
    ISSN: 1432-0622
    Keywords: Gröbner bases ; Polynomial ideals ; Dual bases ; Interpolation ; 0-dimensional schemes
    Source: Springer Online Journal Archives 1860-2000
    Topics: Computer Science , Mathematics , Technology
    Notes: Abstract In this paper we study 0-dimensional polynomial ideals defined by a dual basis, i.e. as the set of polynomials which are in the kernel of a set of linear morphisms from the polynomial ring to the base field. For such ideals, we give polynomial complexity algorithms to compute a Gröbner basis, generalizing the Buchberger-Möller algorithm for computing a basis of an ideal vanishing at a set of points and the FGLM basis conversion algorithm. As an application to Algebraic Geometry, we show how to compute in polynomial time a minimal basis of an ideal of projective points.
    Type of Medium: Electronic Resource
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  • 10
    Electronic Resource
    Electronic Resource
    Springer
    Applicable algebra in engineering, communication and computing 4 (1993), S. 217-230 
    ISSN: 1432-0622
    Keywords: Algebraic variety decomposition ; Gröbner bases ; Systems of nonlinear equations
    Source: Springer Online Journal Archives 1860-2000
    Topics: Computer Science , Mathematics , Technology
    Notes: Abstract This paper deals with systems ofm polynomial equations inn unknown, which have only finitely many solutions. A method is presented which decomposes the solution set into finitely many subsets, each of them given by a system of type $$f_1 \left( {x_1 } \right) = 0,f_2 \left( {x_1 ,x_2 } \right) = 0, \ldots ,f_n \left( {x_1 , \ldots ,x_n } \right) = 0$$ . The main tools for the decomposition are from ideal theory and use symbolical manipulations. For the ideal generated by the polynomials which describe the solution set, a lexicographical Gröbner basis is required. A particular element of this basis allows the decomposition of the solution set. By a recursive application of these decomposition techniques the triangular subsystems are finally obtained. The algorithm gives even for non-finite solution sets often also usable decompositions.
    Type of Medium: Electronic Resource
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