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  • Springer  (2)
  • 1995-1999  (2)
  • 1
    Electronic Resource
    Electronic Resource
    Springer
    Journal of bioenergetics and biomembranes 29 (1997), S. 603-609 
    ISSN: 1573-6881
    Keywords: Membrane potential ; reconstituted plasma membrane vesicles ; oxonol VI fluorescence ; yeast H+-ATPase ; Schizosaccharomyces pombe
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology , Physics
    Notes: Abstract A model of membrane potential-dependent distribution of oxonol VI to estimate the electrical potential difference Δψ across Schizosaccharomyces pombe plasma membrane vesicles (PMV) has been developed. Δψ was generated by the H+-ATPase reconstituted in the PMV. The model treatment was necessary since the usual calibration of the dye fluorescence changes by diffusion potentials (K+ + valinomycin) failed. The model allows for fitting of fluorescence changes at different vesicle and dye concentrations, yielding Δψ in ATP-energized PMV of 80 mV. The described model treatment to estimate Δψ may be applicable for other reconstituted membrane systems.
    Type of Medium: Electronic Resource
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  • 2
    ISSN: 1573-1561
    Keywords: Dictyoptera ; Blattellidae ; Blattella germanica ; German cockroach ; aggregation ; pheromone
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology
    Notes: Abstract Aggregation behavior and reduced locomotory activity in the German cockroach is known to be caused by chemical compounds in the feces. The attractive and/or arrestant efficacy of three relevant substances was tested in first instars by a two-choice aggregation test and in adults with a locomotion compensator apparatus that allows quantification of taste-directed orientation and walking speed as a function of antennal stimulation. The three substances tested were a feces crude extract; a mixture of six carboxylic acids (mix G) out of a total of 29 that were identified in the feces extract and tested as single compounds and in various combinations; and a steroid glucoside denoted as blattellastanoside A, which has been suggested as an aggregation arrestant pheromone in Blattella germanica. With both of our test methods, feces extract and mix G proved to be very attractive, whereas the effects of blattellastanoside A were, if anything, very poor. Possible reasons for discrepancies are discussed.
    Type of Medium: Electronic Resource
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