ISSN:
1573-6881
Keywords:
Membrane potential
;
reconstituted plasma membrane vesicles
;
oxonol VI fluorescence
;
yeast H+-ATPase
;
Schizosaccharomyces pombe
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
,
Chemistry and Pharmacology
,
Physics
Notes:
Abstract A model of membrane potential-dependent distribution of oxonol VI to estimate the electrical potential difference Δψ across Schizosaccharomyces pombe plasma membrane vesicles (PMV) has been developed. Δψ was generated by the H+-ATPase reconstituted in the PMV. The model treatment was necessary since the usual calibration of the dye fluorescence changes by diffusion potentials (K+ + valinomycin) failed. The model allows for fitting of fluorescence changes at different vesicle and dye concentrations, yielding Δψ in ATP-energized PMV of 80 mV. The described model treatment to estimate Δψ may be applicable for other reconstituted membrane systems.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1023/A:1022439203461
