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  • 1
    ISSN: 1573-4919
    Schlagwort(e): fructose-1,6-diphosphate ; membrane permeability ; endothelial cells ; hypoxia
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie , Chemie und Pharmazie , Medizin
    Notizen: Abstract Fructose-1,6-diphosphate (FDP) is a glycolytic intermediate which has been used an intervention in various ischemic conditions for two decades. Yet whether FDP can enter the cell is under constant debate. In this study we examined membrane permeability of FDP in artificial membrane bilayers and in endothelial cells. To examine passive diffusion of FDP through the membrane bilayer, L-a-phosphatidylcholine from egg yolk (Egg PC) (10 mM) multi-lamellar vesicles were created containing different external concentrations of FDP (0, 0.5, 5 and 50 mM). The passive diffusion of FDP into the vesicles was followed spectrophotometrically. The results indicate that FDP diffuses through the membrane bilayer in a dose-dependent fashion. The movement of FDP through Egg PC membrane bilayers was confirmed by measuring the conversion of FDP to dihydroxyacetone-phosphate and the formation of hydrozone. FDP (0, 0.5, 5 or 50 mM) was encapsulated in Egg PC multilamellar vesicles and placed in a solution containing aldolase. In the 5 and 50 mM FDP groups there was a significant increase in dihydroxyacetone/hydrazone indicating that FDP crossed the membrane bilayer intact. We theorized that the passive diffusion of FDP might be due to disruption of the membrane bilayer. To examine this hypothesis, small unilamellar vesicles composed of Egg PC were created in the presence of 60 mM carboxyfluorescein, and the leakage of the sequestered dye was followed upon addition of various concentrations of FDP, fructose, fructose-6-phosphate, or fructose-1-phosphate (0, 5 or 50 mM). These results indicate that increasing concentrations of FDP increase the leakage rate of carboxyfluorescein. In contrast, no concentration of fructose, fructose-6-phosphate, or fructose-1-phosphate resulted in any significant increase in membrane permeability to carboxyfluorescein. To examine whether FDP could pass through cellular membranes, we examined the uptake of 14C-FDP by endothelial cells cultured under hypoxia or normoxia for 4 or 16 h. The uptake of FDP was dose-dependent in both the normoxia and hypoxia treated cells, and was accompanied by no significant loss in endothelial cell viability. Our results demonstrate that FDP can diffuse through membrane bilayers in a dose-dependent manner.
    Materialart: Digitale Medien
    Standort Signatur Einschränkungen Verfügbarkeit
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  • 2
    ISSN: 1573-2576
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Medizin
    Notizen: Abstract Bradykinin and α-thrombin both increase endothelial macromolecular permeability, however the mechanism for this effect is unclear. Human umbilical vein endothelial cell (HUVEC) permeability to human serum albumin was increased by 1 μM α-thrombin (AT) or bradykinin (BK), but the kinetics of the permeability response were different. Intracellular calcium mobilization of HUVEC by AT was increased, yet BK had no effect on intracellular calcium. Distribution of F-actin and content was increased by AT as early as 10 minutes after administration, yet BK had no affect on F-actin when compared to control. We hypothesized that BK may increase HUVEC permeability by producing matrix metalloproteinase-2 (MMP-2). The AT-treated HUVEC produced an intermediate 64 kDa MMP-2, whereas BK-treated HUVEC increased the intermediate 64 kDa MMP-2 and also an active 62 kDa MMP-2. Pre-treatment of the HUVEC with tissue inhibitor of matrix metalloproteinase-2 slightly decreased the AT-induced increase in macromolecular permeability and completely inhibited the BK-induced increase in macromolecular permeability.
    Materialart: Digitale Medien
    Standort Signatur Einschränkungen Verfügbarkeit
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  • 3
    Publikationsdatum: 2021-06-25
    Beschreibung: The interannual-decadal variability of the wintertime mixed layer depths (MLDs) over the North Pacific is investigated from an empirical orthogonal function (EOF) analysis of an ensemble of global ocean reanalyses. The first leading EOF mode represents the interannual MLD anomalies centered in the eastern part of the central mode water formation region in phase opposition with those in the eastern subtropics and the central Alaskan Gyre. This first EOF mode is highly correlated with the Pacific decadal oscillation index on both the interannual and decadal time scales. The second leading EOF mode represents the MLD variability in the subtropical mode water (STMW) formation region and has a good correlation with the wintertime West Pacific (WP) index with time lag of 3 years, suggesting the importance of the oceanic dynamical response to the change in the surface wind field associated with the meridional shifts of the Aleutian Low. The above MLD variabilities are in basic agreement with previous observational and modeling findings. Moreover the reanalysis ensemble provides uncertainty estimates. The interannual MLD anomalies in the first and second EOF modes are consistently represented by the individual reanalyses and the amplitudes of the variabilities generally exceed the ensemble spread of the reanalyses. Besides, the resulting MLD variability indices, spanning the 1948–2012 period, should be helpful for characterizing the North Pacific climate variability. In particular, a 6-year oscillation including the WP teleconnection pattern in the atmosphere and the oceanic MLD variability in the STMW formation region is first detected.
    Beschreibung: Published
    Beschreibung: 891–907
    Beschreibung: 4A. Oceanografia e clima
    Beschreibung: JCR Journal
    Repository-Name: Istituto Nazionale di Geofisica e Vulcanologia (INGV)
    Materialart: article
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  • 4
    Publikationsdatum: 2021-06-25
    Beschreibung: Intercomparison and evaluation of the global ocean surface mixed layer depth (MLD) fields estimated from a suite of major ocean syntheses are conducted. Compared with the reference MLDs calculated from individual profiles, MLDs calculated from monthly mean and gridded profiles show negative biases of 10–20 m in early spring related to the re-stratification process of relatively deep mixed layers. Vertical resolution of profiles also influences the MLD estimation. MLDs are underestimated by approximately 5–7 (14–16) m with the vertical resolution of 25 (50) m when the criterion of potential density exceeding the 10-m value by 0.03 kg m−3 is used for the MLD estimation. Using the larger criterion (0.125 kg m−3) generally reduces the underestimations. In addition, positive biases greater than 100 m are found in wintertime subpolar regions when MLD criteria based on temperature are used. Biases of the reanalyses are due to both model errors and errors related to differences between the assimilation methods. The result shows that these errors are partially cancelled out through the ensemble averaging. Moreover, the bias in the ensemble mean field of the reanalyses is smaller than in the observation-only analyses. This is largely attributed to comparably higher resolutions of the reanalyses. The robust reproduction of both the seasonal cycle and interannual variability by the ensemble mean of the reanalyses indicates a great potential of the ensemble mean MLD field for investigating and monitoring upper ocean processes.
    Beschreibung: Published
    Beschreibung: 753–773
    Beschreibung: 4A. Oceanografia e clima
    Beschreibung: JCR Journal
    Repository-Name: Istituto Nazionale di Geofisica e Vulcanologia (INGV)
    Materialart: article
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  • 5
    Publikationsdatum: 2022-10-26
    Beschreibung: © The Author(s), 2019. This article is distributed under the terms of the Creative Commons Attribution License. The definitive version was published in Heimbach, P., Fukumori, I., Hills, C. N., Ponte, R. M., Stammer, D., Wunsch, C., Campin, J., Cornuelle, B., Fenty, I., Forget, G., Koehl, A., Mazloff, M., Menemenlis, D., Nguyen, A. T., Piecuch, C., Trossman, D., Verdy, A., Wang, O., & Zhang, H. Putting it all together: Adding value to the global ocean and climate observing systems with complete self-consistent ocean state and parameter estimates. Frontiers in Marine Science, 6 (2019):55, doi:10.3389/fmars.2019.00055.
    Beschreibung: In 1999, the consortium on Estimating the Circulation and Climate of the Ocean (ECCO) set out to synthesize the hydrographic data collected by the World Ocean Circulation Experiment (WOCE) and the satellite sea surface height measurements into a complete and coherent description of the ocean, afforded by an ocean general circulation model. Twenty years later, the versatility of ECCO's estimation framework enables the production of global and regional ocean and sea-ice state estimates, that incorporate not only the initial suite of data and its successors, but nearly all data streams available today. New observations include measurements from Argo floats, marine mammal-based hydrography, satellite retrievals of ocean bottom pressure and sea surface salinity, as well as ice-tethered profiled data in polar regions. The framework also produces improved estimates of uncertain inputs, including initial conditions, surface atmospheric state variables, and mixing parameters. The freely available state estimates and related efforts are property-conserving, allowing closed budget calculations that are a requisite to detect, quantify, and understand the evolution of climate-relevant signals, as mandated by the Coupled Model Intercomparison Project Phase 6 (CMIP6) protocol. The solutions can be reproduced by users through provision of the underlying modeling and assimilation machinery. Regional efforts have spun off that offer increased spatial resolution to better resolve relevant processes. Emerging foci of ECCO are on a global sea level changes, in particular contributions from polar ice sheets, and the increased use of biogeochemical and ecosystem data to constrain global cycles of carbon, nitrogen and oxygen. Challenges in the coming decade include provision of uncertainties, informing observing system design, globally increased resolution, and moving toward a coupled Earth system estimation with consistent momentum, heat and freshwater fluxes between the ocean, atmosphere, cryosphere and land.
    Beschreibung: Major support for ECCO is provided by NASA's Physical Oceanography program via a contract to JPL/Caltech, with additional support through NASA's Modeling, Analysis and Prediction program, the Cryosphere Science program, and the Computational Modeling and Cyberinfrastructure program. Supplemental funding was obtained throughout the years via standard grants to individual team members from NSF, NOAA, and ONR.
    Schlagwort(e): ECCO ; Global ocean inverse modeling ; Optimal state and parameter estimation ; Adjoint method ; Ocean observations ; Coupled Earth system data assimilation ; Ocean reanalysis ; Global ocean circulation
    Repository-Name: Woods Hole Open Access Server
    Materialart: Article
    Standort Signatur Einschränkungen Verfügbarkeit
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  • 6
    Publikationsdatum: 2022-10-26
    Beschreibung: © The Author(s), 2022. This article is distributed under the terms of the Creative Commons Attribution License. The definitive version was published in Chandanpurkar, H. A., Lee, T., Wang, X., Zhang, H., Fournier, S., Fenty, I., Fukumori, I., Menemenlis, D., Piecuch, C. G., Reager, J. T., Wang, O., & Worden, J. Influence of nonseasonal river discharge on sea surface salinity and height. Journal of Advances in Modeling Earth Systems, 14(2), (2022): e2021MS002715, https://doi.org/10.1029/2021MS002715.
    Beschreibung: River discharge influences ocean dynamics and biogeochemistry. Due to the lack of a systematic, up-to-date global measurement network for river discharge, global ocean models typically use seasonal discharge climatology as forcing. This compromises the simulated nonseasonal variation (the deviation from seasonal climatology) of the ocean near river plumes and undermines their usefulness for interdisciplinary research. Recently, a reanalysis-based daily varying global discharge data set was developed, providing the first opportunity to quantify nonseasonal discharge effects on global ocean models. Here we use this data set to force a global ocean model for the 1992–2017 period. We contrast this experiment with another experiment (with identical atmospheric forcings) forced by seasonal climatology from the same discharge data set to isolate nonseasonal discharge effects, focusing on sea surface salinity (SSS) and sea surface height (SSH). Near major river mouths, nonseasonal discharge causes standard deviations in SSS (SSH) of 1.3–3 practical salinity unit (1–2.7 cm). The inclusion of nonseasonal discharge results in notable improvement of model SSS against satellite SSS near most of the tropical-to-midlatitude river mouths and minor improvement of model SSH against satellite or in-situ SSH near some of the river mouths. SSH changes associated with nonseasonal discharge can be explained by salinity effects on halosteric height and estimated accurately through the associated SSS changes. A recent theory predicting river discharge impact on SSH is found to perform reasonably well overall but underestimates the impact on SSH around the global ocean and has limited skill when applied to rivers near the equator and in the Arctic Ocean.
    Beschreibung: This research was carried out in part at the Jet Propulsion Laboratory, California Institute of Technology, under a contract with the National Aeronautics and Space Administration (80NM0018D0004) with support from the Physical Oceanography (PO) and Modeling, Analysis, and Prediction (MAP) Programs. High-end computing resources for the numerical simulation were provided by the NASA Advanced Supercomputing Division at the Ames Research Center.
    Schlagwort(e): River discharge ; Sea surface salinity ; Sea surface height
    Repository-Name: Woods Hole Open Access Server
    Materialart: Article
    Standort Signatur Einschränkungen Verfügbarkeit
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  • 7
    Publikationsdatum: 2022-11-04
    Beschreibung: Author Posting. © American Meteorological Society, 2022. This article is posted here by permission of American Meteorological Society for personal use, not for redistribution. The definitive version was published in Journal of Physical Oceanography 52(11), (2022): 2627-2641, https://doi.org/10.1175/jpo-d-22-0090.1.
    Beschreibung: Changes in dynamic manometric sea level ζm represent mass-related sea level changes associated with ocean circulation and climate. We use twin model experiments to quantify magnitudes and spatiotemporal scales of ζm variability caused by barometric pressure pa loading at long periods (≳1 month) and large scales (≳300km) relevant to Gravity Recovery and Climate Experiment (GRACE) ocean data. Loading by pa drives basin-scale monthly ζm variability with magnitudes as large as a few centimeters. Largest ζm signals occur over abyssal plains, on the shelf, and in marginal seas. Correlation patterns of modeled ζm are determined by continental coasts and H/f contours (H is ocean depth and f is Coriolis parameter). On average, ζm signals forced by pa represent departures of ≲10% and ≲1% from the inverted-barometer effect ζib on monthly and annual periods, respectively. Basic magnitudes, spatial patterns, and spectral behaviors of ζm from the model are consistent with scaling arguments from barotropic potential vorticity conservation. We also compare ζm from the model driven by pa to ζm from GRACE observations. Modeled and observed ζm are significantly correlated across parts of the tropical and extratropical oceans, on shelf and slope regions, and in marginal seas. Ratios of modeled to observed ζm magnitudes are as large as ∼0.2 (largest in the Arctic Ocean) and qualitatively agree with analytical theory for the gain of the transfer function between ζm forced by pa and wind stress. Results demonstrate that pa loading is a secondary but nevertheless important contributor to monthly mass variability from GRACE over the ocean.
    Beschreibung: The authors acknowledge support from the National Aeronautics and Space Administration through the GRACE Follow-On Science Team (Grant 80NSSC20K0728) and the Sea Level Change Team (Grant 80NSSC20K1241). The contribution from I. F. and O. W. represents research carried out at the Jet Propulsion Laboratory, California Institute of Technology, under a contract with the National Aeronautics and Space Administration (Grant 80NM0018D0004).
    Schlagwort(e): Barotropic flows ; Large-scale motions ; Ocean circulation ; Planetary waves ; Potential vorticity ; Sea level
    Repository-Name: Woods Hole Open Access Server
    Materialart: Article
    Standort Signatur Einschränkungen Verfügbarkeit
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  • 8
    Publikationsdatum: 2022-10-26
    Beschreibung: © The Author(s), 2022. This article is distributed under the terms of the Creative Commons Attribution License. The definitive version was published in Wang, O., Lee, T., Piecuch, C., Fukumori, I., Fenty, I., Frederikse, T., Menemenlis, D., Ponte, R., & Zhang, H. Local and remote forcing of interannual sea‐level variability at Nantucket Island. Journal of Geophysical Research: Oceans, 127(6), (2022): e2021JC018275, https://doi.org/10.1029/2021jc018275.
    Beschreibung: The relative contributions of local and remote wind stress and air-sea buoyancy forcing to sea-level variations along the East Coast of the United States are not well quantified, hindering the understanding of sea-level predictability there. Here, we use an adjoint sensitivity analysis together with an Estimating the Circulation and Climate of the Ocean (ECCO) ocean state estimate to establish the causality of interannual variations in Nantucket dynamic sea level. Wind forcing explains 67% of the Nantucket interannual sea-level variance, while wind and buoyancy forcing together explain 97% of the variance. Wind stress contribution is near-local, primarily from the New England shelf northeast of Nantucket. We disprove a previous hypothesis about Labrador Sea wind stress being an important driver of Nantucket sea-level variations. Buoyancy forcing, as important as wind stress in some years, includes local contributions as well as remote contributions from the subpolar North Atlantic that influence Nantucket sea level a few years later. Our rigorous adjoint-based analysis corroborates previous correlation-based studies indicating that sea-level variations in the subpolar gyre and along the United States northeast coast can both be influenced by subpolar buoyancy forcing. Forward perturbation experiments further indicate remote buoyancy forcing affects Nantucket sea level mostly through slow advective processes, although coastally trapped waves can cause rapid Nantucket sea level response within a few weeks.
    Beschreibung: This research was carried out in part at the Jet Propulsion Laboratory, California Institute of Technology, under a contract with the National Aeronautics and Space Administration (80NM0018D0004). CGP was supported by NASA Sea Level Change Team awards 80NSSC20K1241 and 80NM0018D0004.
    Schlagwort(e): Sea level ; Adjoint sensitivity ; Forcing mechanism
    Repository-Name: Woods Hole Open Access Server
    Materialart: Article
    Standort Signatur Einschränkungen Verfügbarkeit
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  • 9
    Publikationsdatum: 2014-11-11
    Beschreibung: The Journal of Organic Chemistry DOI: 10.1021/jo5020307
    Print ISSN: 0022-3263
    Digitale ISSN: 1520-6904
    Thema: Chemie und Pharmazie
    Standort Signatur Einschränkungen Verfügbarkeit
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