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  • 1
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    Dissolved organic matter composition derived from FT-ICR-MS analyses during HEINCKE cruise HE361
    PANGAEA
    In:  Supplement to: Osterholz, Helena; Singer, Gabriel; Wemheuer, Bernd; Daniel, Rolf; Simon, Meinhard; Niggemann, Jutta; Dittmar, Thorsten (2016): Deciphering associations between dissolved organic molecules and bacterial communities in a pelagic marine system. The ISME Journal, https://doi.org/10.1038/ismej.2015.231
    Details
    Publication Date: 2023-05-12
    Description: Dissolved organic matter (DOM) is the main substrate and energy source for heterotrophic bacterioplankton. To understand the interactions between DOM and the bacterial community (BC), it is important to identify the key factors on both sides in detail, chemically distinct moieties in DOM and the various bacterial taxa. Next-generation sequencing facilitates the classification of millions of reads of environmental DNA and RNA amplicons and ultrahigh-resolution mass spectrometry yields up to 10,000 DOM molecular formulae in a marine water sample. Linking this detailed biological and chemical information is a crucial first step toward a mechanistic understanding of the role of microorganisms in the marine carbon cycle. In this study, we interpreted the complex microbiological and molecular information via a novel combination of multivariate statistics. We were able to reveal distinct relationships between the key factors of organic matter cycling along a latitudinal transect across the North Sea. Total BC and DOM composition were mainly driven by mixing of distinct water masses and presumably retain their respective terrigenous imprint on similar timescales on their way through the North Sea. The active microbial community, however, was rather influenced by local events and correlated with specific DOM molecular formulae indicative of compounds that are easily degradable. These trends were most pronounced on the highest resolved level, that is, operationally defined 'species', reflecting the functional diversity of microorganisms at high taxonomic resolution.
    Type: Dataset
    Format: application/zip, 2 datasets
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    DATA PANGAEA
  • 2
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    Dissolved organic matter composition given as FT-ICR-MS peak intensities measured on water bottle samples during HEINCKE cruise HE361 (2016)
    PANGAEA
    Details
    Publication Date: 2024-01-12
    Keywords: CTD/Rosette; CTD-RO; Date/Time of event; DEPTH, water; Elevation of event; Event label; Fourier transform ion cyclotron resonance mass spectrometry (FT-ICR-MS); HE361; HE361/001-1; HE361/002-1; HE361/003-1; HE361/005-1; HE361/006-1; HE361/007-1; HE361/008-1; HE361/010-1; HE361/012-1; HE361/013-1; HE361/014-1; HE361/015-1; HE361/016-1; HE361/027-1; HE361/032-1; HE361/033-1; HE361/034-1; HE361/035-1; Heincke; Latitude of event; Longitude of event; Molecular formula; North Sea; Peak intensity; Skagerrak
    Type: Dataset
    Format: text/tab-separated-values, 588780 data points
    Location Call Number Limitation Availability
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    DATA PANGAEA
  • 3
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    Dissolved organic matter composition derived from FT-ICR-MS analyses (2016)
    PANGAEA
    Details
    Publication Date: 2024-01-12
    Keywords: Calculated; DEPTH, water; HE361; HE361_DOM; Heincke; Hydrogen/Carbon ratio; Mass-to-charge ratio; Molecular formula; MULT; Multiple investigations; Oxygen/Carbon ratio
    Type: Dataset
    Format: text/tab-separated-values, 26168 data points
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    DATA PANGAEA
  • 4
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    Water isotope values in shore samples collected in March and July 2020 from northeastern German lake systems (2021)
    PANGAEA
    Details
    Publication Date: 2024-03-22
    Description: Water isotopes (δ2H and δ18O) were analyzed in samples collected in lakes associated to major riverine systems in northeastern Germany throughout 2020. This sub-dataset is derived from water samples collected from lake shores. Samples were taken in March and July 2020 with a pipette from 40-60 cm depth below water surface and directly transferred into a measurement vial. Stable isotope analysis was conducted at IGB Berlin, using a Picarro L2130-i cavity ring-down spectrometer. The data give information about the seasonal isotope amplitude in the sampled lakes and about spatial isotope variability in different branches of the associated riverine systems.
    Keywords: Breiter_Luzin; Breiter Luzin, Germany; Canower_See; Canower See, Germany; Cavity ring-down spectroscopy (CRDS), L21301i, Picarro Inc.; CONNECT; Connectivity and synchronization of lake ecosystems in space and time; Daemeritzsee; Dahme; Dahme, Germany; Dämeritzsee, Germany; DATE/TIME; DEPTH, water; Event label; Feldberger_Haussee; Feldberger Haussee, Germany; GEPRIS_418096356; Große_Krampe; Große Krampe, Germany; Großer_Priepertsee; Großer_See-Fürstenwerder; Großer Priepertsee, Germany; Großer See, Fürstenwerder, Germany; Havel; Influence of environmental factors onto the hydrogen isotopic signature of aquatic plants; Kleiner Müggelsee, Germany; Koelpinsee; Kölpinsee , Germany; Krewitzsee; Krewitzsee, Germany; Labussee; Labussee, Germany; Latitude of event; Longitude of event; Mellensee, Funkenhagen, Germany; Mellensee-Funkenhagen; Mueggelsee_a; Mueggelsee_b; Mueggelsee_inflow; Mueggelsee_outflow; Mueritz; Müggelsee, Germany; Müritz; Müritz, Germany; Oberrucker_See_Gr._Lanke; Oberrucker See (Gr. Lanke), Germany; Peetschsee; Peetschsee, Germany; Pipette; Raetzsee; Rätzsee, Germany; Schmaler_Luzin_a; Schmaler_Luzin_b; Schmaler Luzin, Germany; Schwarzer_See-Mirow; Schwarzer See, Mirow, Germany; Spree; Spree_a; Spree_b; Spree, Germany; Suckower_Haussee; Suckower Haussee, Germany; Ucker; Vilzsee; Vilzsee, Germany; Wrechener_See; Wrechener See, Germany; Zethnersee; Zethnersee, Germany; Zotzensee_a; Zotzensee_b; Zotzensee, Germany; δ18O; δ18O, water; δ18O, water, standard deviation; δ2H; δ Deuterium, water; δ Deuterium, water, standard deviation
    Type: Dataset
    Format: text/tab-separated-values, 204 data points
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    DATA PANGAEA
  • 5
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    Water isotope values in time series samples (March - October 2020) collected at buoys deployed in northeastern German lake systems (2021)
    PANGAEA
    Details
    Publication Date: 2024-03-18
    Description: Water isotopes (δ2H and δ18O) were analyzed in samples collected in lakes associated to major riverine systems in northeastern Germany throughout 2020. This sub-dataset is derived from water samples taken at buoys temporarily installed in deep parts of the lake. Samples were taken monthly to bimonthly from March to October 2020. A Limnos water sampler was used to obtain samples from 1 m below water surface. Isotope analysis was conducted at IGB Berlin, using a Picarro L2130-i cavity ring-down spectrometer. Water temperatures were measured in similar depths with an Aqua TROLL 600 multiparameter sonde (In-Situ, Fort Collins, CO, USA). The data give information about the seasonal isotope amplitude in the sampled lakes and about spatial isotope variability in different branches of the associated riverine systems.
    Keywords: Breiter_Luzin_Buoy; Breiter Luzin, Germany; Cavity ring-down spectroscopy (CRDS), L2130-i, Picarro Inc.; Comment; CONNECT; Connectivity and synchronization of lake ecosystems in space and time; CT-probe Aqua TROLL 100; Dahme; DATE/TIME; Depth, bottom/max; DEPTH, water; Ellbogensee_Buoy; Ellbogensee, Germany; Event label; Feldberger_Haussee_Buoy; Feldberger Haussee, Germany; GEPRIS_418096356; Gr_Paelitzsee_north_Buoy; Großer_Labussee_Buoy; Großer_Lychensee_Buoy; Großer_Priepertsee_Buoy; Großer Labussee, Germany; Großer Lychensee, Germany; Großer Priepertsee, Germany; Groß Pälitzsee (north), Germany; Havel; Influence of environmental factors onto the hydrogen isotopic signature of aquatic plants; Kl_Paelitzsee_east_Buoy; Klein Pälitzsee (east), Germany; Labussee_Buoy; Labussee, Germany; LIMNOS water sampler; LIMNOSWS; Müritz; Röblinsee, Germany; Roeblinsee_Buoy; Schmaler_Luzin_Buoy; Schmaler Luzin, Germany; Spree; Stechlinsee_Buoy; Stechlinsee, Germany; Stolpsee_Buoy; Stolpsee, Germany; Temperature, water; Ucker; Useriner_See_Buoy; Useriner See, Germany; Vilzsee_Buoy; Vilzsee, Germany; Woblitzsee_Buoy; Woblitzsee, Germany; Zierker_See_Buoy; Zierker See, Germany; Ziernsee_Buoy; Ziernsee, Germany; Zotzensee_Buoy; Zotzensee, Germany; δ18O; δ18O, water; δ18O, water, standard deviation; δ2H; δ Deuterium, water; δ Deuterium, water, standard deviation
    Type: Dataset
    Format: text/tab-separated-values, 781 data points
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    DATA PANGAEA
  • 6
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    Water isotope values from a spatial campaign conducted in four northeastern German lakes in September 2020 (2021)
    PANGAEA
    Details
    Publication Date: 2024-03-18
    Description: Water isotopes (δ2H and δ18O) were analyzed in samples collected in lakes associated to major riverine systems in northeastern Germany throughout 2020. This sub-dataset is derived from water samples taken at multiple spatially distributed spots in four selected lakes. A Limnos water sampler was used to obtain samples from 1 m below water surface on 29th and 30th September 2020. Isotope analysis was conducted at IGB Berlin, using a Picarro L2130-i cavity ring-down spectrometer.
    Keywords: Cavity ring-down spectroscopy (CRDS), L2130-i, Picarro Inc.; CONNECT; Connectivity and synchronization of lake ecosystems in space and time; Dahme; Depth, bottom/max; DEPTH, water; Depth of Secchi Disk; Ellbogensee_1; Ellbogensee_2; Ellbogensee_3; Ellbogensee_4; Ellbogensee_5; Ellbogensee_6; Ellbogensee, Germany; Event label; GEPRIS_418096356; Großer_Priepertsee_1; Großer_Priepertsee_2; Großer_Priepertsee_3; Großer_Priepertsee_4; Großer_Priepertsee_5; Großer_Priepertsee_6; Großer_Priepertsee_7; Großer Priepertsee, Germany; Havel; Influence of environmental factors onto the hydrogen isotopic signature of aquatic plants; Location; Müritz; Röblinsee, Germany; Roeblinsee_1; Roeblinsee_2; Roeblinsee_3; Roeblinsee_4; Roeblinsee_5; Roeblinsee_6; Spree; Ucker; Zierker_See_1; Zierker_See_2; Zierker_See_3; Zierker_See_4; Zierker_See_5; Zierker_See_6; Zierker See, Germany; δ18O; δ18O, water; δ18O, water, standard deviation; δ2H; δ Deuterium, water; δ Deuterium, water, standard deviation
    Type: Dataset
    Format: text/tab-separated-values, 171 data points
    Location Call Number Limitation Availability
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    DATA PANGAEA
  • 7
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    Water isotope values from northeastern German lake systems 2020 et seq (2021)
    PANGAEA
    Details
    Publication Date: 2024-04-23
    Description: Water isotopes (δ2H and δ18O) were analyzed in samples collected in lakes associated to major riverine systems in northeastern Germany throughout 2020. The dataset is derived from water samples taken at a) lake shores (sampled in March and July 2020); b) buoys temporarily installed in deep parts of the lake (sampled monthly from March to October 2020); c) multiple spatially distributed spots in four selected lakes (in September 2020); d) the outflow of Müggelsee (sampled biweekly from March 2020 to January 2021). At shores, water was sampled with a pipette from 40-60 cm below water surface and directly transferred into a measurement vial, while at buoys a Limnos water sampler was used to obtain samples from 1 m below surface. Isotope analysis was conducted at IGB Berlin, using a Picarro L2130-i cavity ring-down spectrometer. The data give information about the seasonal isotope amplitude in the sampled lakes and about spatial isotope variability in different branches of the associated riverine systems.
    Keywords: CONNECT; Connectivity and synchronization of lake ecosystems in space and time; Dahme; GEPRIS_418096356; Havel; Influence of environmental factors onto the hydrogen isotopic signature of aquatic plants; Müritz; Spree; Ucker; δ18O; δ2H
    Type: Dataset
    Format: application/zip, 6 datasets
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    DATA PANGAEA
  • 8
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    Dissolved organic matter composition from a large scale enclosure experiment (2020)
    PANGAEA
    Details
    Publication Date: 2024-04-20
    Description: During the summer of 2015, we reproduced three levels of browning and seven levels of nutrients using water from lake Stechlin (North-East Germany). We applied ultra-high-resolution mass-spectrometry and dissolved organic matter optical properties to retrieve the composition of the DOM at different levels of resolutions. Using a network analysis approach, we found that molecular formulas clustering together share a common origin.
    Keywords: Brownification; DOM; Enclosure experiment; FT-ICR-MS; Germany; Lake_Stechlin; nutrients
    Type: Dataset
    Format: text/plain, 3.6 MBytes
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    DATA PANGAEA
  • 9
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    Improved Mass Accuracy and Isotope Confirmation through Alignment of Ultrahigh-Resolution Mass Spectra of Complex Natural Mixtures (2019)
    AMER CHEMICAL SOC
    In:  EPIC3Analytical Chemistry, AMER CHEMICAL SOC, 92(2), pp. 2558-2565, ISSN: 0003-2700
    Details
    Publication Date: 2020-05-27
    Description: Fourier-transform ion cyclotron resonance mass spectrometry (FT-ICR-MS) is one of the state-of-the-art methods to analyze complex natural organic mixtures. The precision of detected masses is crucial for molecular formula attribution. Random errors can be reduced by averaging multiple measurements of the same mass, but because of limited availability of ultrahigh-resolution mass spectrometers, most studies cannot afford analyzing each sample multiple times. Here we show that random errors can be eliminated also by averaging mass spectral data from independent environmental samples. By averaging the spectra of 30 samples analyzed on our 15 T instrument we reach a mass precision comparable to a single spectrum of a 21 T instrument. We also show that it is possible to accurately and reproducibly determine isotope ratios with FT-ICR-MS. Intensity ratios of isotopologues were improved to a degree that measured deviations were within the range of natural isotope fractionation effects. In analogy to δ13C in environmental studies, we propose Δ13C as an analytical measure for isotope ratio deviances instead of widely employed C deviances. In conclusion, here we present a simple tool, extensible to Orbitrap-based mass spectrometers, for postdetection data processing that significantly improves mass accuracy and the precision of intensity ratios of isotopologues at no extra cost.
    Repository Name: EPIC Alfred Wegener Institut
    Type: Article , peerRev
    Format: application/pdf
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    PAPER (OA)
  • 10
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    Planktotrons: A novel indoor mesocosm facility for aquatic biodiversity and food web research (2017)
    AMER SOC LIMNOLOGY OCEANOGRAPHY
    In:  EPIC3Limnology and Oceanography-Methods, AMER SOC LIMNOLOGY OCEANOGRAPHY, 15(7), pp. 663-677, ISSN: 1541-5856
    Details
    Publication Date: 2017-08-29
    Description: We established a new indoor mesocosm facility, 12 fully controlled “Planktotrons”, designed to conduct marine and freshwater experiments for biodiversity and food web approaches using natural or artificial, benthic or planktonic communities. The Planktotrons are a unique and custom-tailored facility allowing long-term experiments. Wall growth can be inhibited by a rotating gate paddle with silicone lips. Additionally, temperature and light intensity are individually controllable for each Planktotron and the large volume (600 L) enables high-frequency or volume-intense measurements. In a pilot freshwater experiment various trophic levels of a pelagic food web were maintained for up to 90 d. First, an artificially assembled phytoplankton community of 11 species was inoculated in all Planktotrons. After 22 d, two ciliates were added to all, and three Daphnia species were added to six Planktotrons. After 72 d, dissolved organic matter (DOM, an alkaline soil extract) was added as an external disturbance to six of the 12 Planktotrons, involving three Planktotrons stocked with Daphnia and three without, respectively. We demonstrate the suitability of the Planktotrons for food web and biodiversity research. Variation among replicated Planktotrons (n = 3 minimum) did not differ from other laboratory systems and field experiments. We investigated population dynamics and interactions among the different trophic levels, and found them affected by the sequence of ciliate and Daphnia addition and the disturbance caused by addition of DOM.
    Repository Name: EPIC Alfred Wegener Institut
    Type: Article , isiRev
    Format: application/pdf
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