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  • 1
    Online Resource
    Online Resource
    Plant Cell, Tissue and Organ Culture : Fundamental Methods. (1995)
    Berlin, Heidelberg :Springer Berlin / Heidelberg,
    Details
    Keywords: Cells, Cultured. ; Tissue culture. ; Plants. ; Electronic books.
    Type of Medium: Online Resource
    Pages: 1 online resource (367 pages)
    Edition: 1st ed.
    ISBN: 9783642790485
    Series Statement: Springer Lab Manuals Series
    URL: https://ebookcentral.proquest.com/lib/geomar/detail.action?docID=6500395
    Language: English
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    GEOMAR EBL
  • 2
    Electronic Resource
    Electronic Resource
    Drugs in crops (continued) (2004)
    [s.l.] : Nature Publishing Group
    Nature biotechnology 22 (2004), S. 655-656 
    Details
    ISSN: 1546-1696
    Source: Nature Archives 1869 - 2009
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: [Auszug] To the editor: Your recent editorial concerning the production of pharmaceuticals in food crops (Nat. Biotechnol. 22, 133, 2004) raises some important issues that are being, and will continue to be, addressed by the molecular farming industry and government regulators. All parties involved agree ...
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1038/nbt0604-655
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    Paper (German National Licenses)
    Fulltext
  • 3
    Electronic Resource
    Electronic Resource
    De novo shoot organogenesis of Pinus eldarica Medw. in vitro (1987)
    Springer
    Plant cell reports 6 (1987), S. 167-171 
    Details
    ISSN: 1432-203X
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Semi-thin section microscopy was used to evaluate callus formation and subsequent shoot regeneration in the Afghan pine, Pinus eldarica, as correlated to macro-photography of the same processes. Evidence showed the development of unorganized callus required three to six months. Observations over the following two years of culture revealed that regeneration of buds involved induction of subsurface reorganization in the tissues. Buds emerged through the surface of the callus later during development. Evidence indicated regeneration was de novo in origin and proceeded by the mode of shoot organogenesis. A single shoot was adventitiously rooted with continuous vascular connection between shoot and root.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00268469
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    Paper (German National Licenses)
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  • 4
    Electronic Resource
    Electronic Resource
    De novo shoot organogenesis of Pinus eldarica Medw. in vitro (1987)
    Springer
    Plant cell reports 6 (1987), S. 163-166 
    Details
    ISSN: 1432-203X
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Seedling-derived explants of the Afghan pine, Pinus eldarica, were cultured in a triplicate experiment to produce callus that was serially subcultured for up to three years. Callus was removed at various times and induced to regenerate shoots by de novo organogenesis. The shoot regeneration process involved the identification of four discrete developmental steps, each requiring a separate cultural manipulation. In one case a regenerated shoot was induced to root following an auxin pulse treatment. Induction and limited development of buds in callus derived from mature-tree explants was also achieved. This is the first reproducible system for shoot regeneration from long-term callus cultures of a conifer.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00268468
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    Paper (German National Licenses)
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  • 5
    Electronic Resource
    Electronic Resource
    High efficiency Agrobacterium-mediated transformation of Lycopersicon based on conditions favorable for regeneration (1987)
    Springer
    Plant cell reports 6 (1987), S. 105-108 
    Details
    ISSN: 1432-203X
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract A successful Agrobacterium-mediated transformation system involving a disarmed Ti plasmid is composed of two stages: transformation of cells and recovery of transformed plants. A tissue transformation system with 34% efficiency was developed using stem segments of the interspecific tomato hybrid Lycopersicon esculentum × L. pennellii. This transformation system emphasizes three factors favoring the recovery of transformed plants: 1) promotion of cell division activity at the inoculation site with kinetin in the incubation medium, 2) promotion of adventitious bud initiation by using organized tissue explants in culture, and 3) application of selection at the shoot development stage of adventitious regeneration.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00276664
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    Paper (German National Licenses)
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  • 6
    Electronic Resource
    Electronic Resource
    Evidence for the occurrence of polyamine oxidase in the dicotyledonous plant Medicago sativa L. (alfalfa) (1991)
    Springer
    Plant cell reports 10 (1991), S. 550-554 
    Details
    ISSN: 1432-203X
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Polyamine oxidase (EC 1.5.3.3) activity has not been detected previously in cells of dicotyledonous plants, although it has been characterized extensively in monocotyledonous plants. Evidence is presented in this report for the occurrence of polyamine oxidase in dialyzed crude extracts of the dicotyledonous plant, Medicago sativa L. (alfalfa). Three enzyme assays were used to quantitate the formation of the three products of the reaction catalyzed by polyamine oxidase. 1-Pyrroline formation was measured colorimetrically as a yellow quinazolinium complex with o-aminobenzaldehyde. Hydrogen peroxide formation was measured spectrophotometrically with a coupled peroxidase assay system by peroxidative oxidation of guaiacol. [3H]1,3-Diaminopropane formation was measured by using [1,8-3H]spermidine as the substrate and separating the radiolabelled reaction product from the substrate by paper electrophoresis. This latter assay provided evidence that a polyamine oxidase of type [EC 1.5.3.3] catalyzed the cleavage reaction between a secondary nitrogen atom and an adjacent carbon of the butyl moiety of spermidine. Significant polyamine oxidase activity was detected in floral tissues, cortex tissues of the root, young leaves, and young germinated seedlings of alfalfa. The occurrence of polyamine oxidase in alfalfa accounts for the formation of the essential substrate, 1,3-diaminopropane, required for the biosynthesis of the uncommon polyamines, norspermidine and norspermine, which we have recently detected in alfalfa.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00232509
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    Paper (German National Licenses)
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  • 7
    Electronic Resource
    Electronic Resource
    Somatic embryogenesis and plant regeneration from zygotic embryo explants in mexican weeping bamboo, Otatea acuminata aztecorum (1992)
    Springer
    Plant cell reports 11 (1992), S. 257-261 
    Details
    ISSN: 1432-203X
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract An efficient protocol has been developed for the in vitro propagation of Mexican Weeping Bamboo through somatic embryogenesis from zygotic embryo explants. Mature seeds and excised embryos were cultured in the light or in the dark on both Murashige and Skoog's and Gamborg's B5 basal media with various supplements. Optimal somatic embryogenesis and plant regeneration were obtained by culture in the dark on Murashige and Skoog's basal medium supplemented with 3 mg/1 2,4-dichlorophenoxyacetic acid, 0.5 mg/1 6-benzylaminopurine and 2.0% sucrose. More than 95% of the germinating somatic embryos developed shoots and roots, and were transferred to soil with 85% success.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00235077
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    Paper (German National Licenses)
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  • 8
    Electronic Resource
    Electronic Resource
    Regeneration of shoots from cell suspension-derived protoplasts ofAllium cepa (1995)
    Springer
    Plant cell reports 15 (1995), S. 8-11 
    Details
    ISSN: 1432-203X
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Callus from pre-selected regenerating lines ofAllium cepa andA. fistulosum were used to initiate cell suspensions. Small clusters of callus selected for greater friability were placed into BDS liquid medium containing 2,4-dichlorophenoxyacetic acid (2,4-D) and 6-benzylaminopurine (BA), and were subcultured biweekly. Rapidly growing, finely dispersed lines were used for protoplast isolation. The highest yields came from 3–4 month old cell suspension lines. Protoplasts were cultured in modified K8P liquid medium. Microcalli recovery depended on the number of weeks the cell suspension had been in culture with highest recovery from 4–5 month old cell suspensions. Microcalli were moved to semisolid media when they were approximately 2 mm in diameter. After 4–6 weeks, embryogenic calli thus recovered were moved to variations of standard onion regeneration media containing picloram and BA. Elongating shoots were obtained from up to 88 % of the microcalli of one line, and 40–50 % of the shoots were further multiplied in culture.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01690243
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    Paper (German National Licenses)
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  • 9
    Electronic Resource
    Electronic Resource
    Organogenesis in pepper tissue cultures (1985)
    Springer
    Plant cell, tissue and organ culture 4 (1985), S. 261-269 
    Details
    ISSN: 1573-5044
    Keywords: pepper ; Capsicum annuum L. ; shoot organogenesis ; root organogenesis ; regeneration
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Knowledge concerning in vitro growth and developmental responses of bell and chile peppers (Capsicum annuum L.) has been limited. Shoot and root organogenesis in cultures of seedling explants was restricted to primary cultures or those less than three months old under 12-and 16-h photoperiod at 25°C. Shoot organogenesis was extended to 5 months under continuous light at 25°C, and to 8 months under continuous light at 28.5°C. Murashige and Skoog basal media containing 0.05mg/l each of IAA and BA promoted shoot elongation and rooting of some explant sources, while 0.05-4 mg/l IAA with 10–50 mg/l BA promoted adventitious shoot bud formation. Glucose was superior to sucrose as the carbon source. Leaf discs collected from greenhouse-grown plants regenerated shoots for at least 2 months. Incubation environment, carbon source, explant source, growth regulator treatment and passage number were not independent variables as demonstrated by statistical analysis. The plant regeneration techniques described here have useful but limited applications, not extending to unorganized callus or cell suspension cultures.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00040200
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    Paper (German National Licenses)
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  • 10
    Electronic Resource
    Electronic Resource
    Induction and development of somatic embryos from cell suspension cultures of soybean (1981)
    Springer
    Plant cell, tissue and organ culture 1 (1981), S. 123-129 
    Details
    ISSN: 1573-5044
    Keywords: Glycine max (L.) Merr. ; in vitro ; plant regeneration ; growth regulators
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Glycine max (L.) Merr. (soybean) andGlycine soja Sieb. and Zucc. cell suspension cultures were grown and used as inoculum sources for growing callus on agar-solidified nutrient media. Concentrations and chemical forms of the growth regulators in liquid and solidified media were altered in an attempt to achieve in vitro plant regeneration. Numerous embryoids, particularly ofG. soja, were produced on basal nutrient media supplemented with 100 ppm casein hydrolysate, 0.1 μM abscisic acid, 2.25 μM 2,4-dichlorophenoxyacetic acid, and 15 μM adenine or 0.46 μM kinetin. Often the roots of the embryoids elongated. This was enhanced in the presence of an inhibitor of gibberellin synthesis (1 to 20 μM Amo 1618). Callus recovered from aG. soja suspension culture produced one shoot structure when grown on a solid medium containing 0.2 μM Amo 1618 and 80 μM glutathione. The shoot structure consisted of two distinct buds, one producing two leaves. The shoot did not develop into a plant. Although regeneration of soybean plants was not achieved, these observations suggest that it may be achievable.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF02318911
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