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  • 1
    Electronic Resource
    Electronic Resource
    Effects of six-carbon aldehydes and alcohols on bacterial proliferation (1993)
    s.l. : American Chemical Society
    Journal of agricultural and food chemistry 41 (1993), S. 506-510 
    Details
    ISSN: 1520-5118
    Source: ACS Legacy Archives
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition , Process Engineering, Biotechnology, Nutrition Technology
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1021/jf00027a030
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  • 2
    Electronic Resource
    Electronic Resource
    Alteration of the synthesis of lipoxygenase in the early stages of soybean cotyledon culture (1988)
    Oxford, UK : Blackwell Publishing Ltd
    Physiologia plantarum 72 (1988), S. 0 
    Details
    ISSN: 1399-3054
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Notes: A detailed study of lipoxygenase (EC 1.13.11.12) synthesis in cotyledons of soybean [Glycine max (L.) Merr. cv. Century] cultured in vitro for up to 40 h showed that synthesis of this protein, measured by in vivo [35S]-methionine labelling in connection with immunological methods and cell-free translation of mRNA, underwent a large transient reduction in the first 4 h of culturing and gradually increased in the following 36 h. Northern blot hybridizations with lipoxygenase cDNA clones showed that the decrease in translational activity was the consequence of a considerable reduction in lipoxygenase mRNA in the cotyledons. From these results we conclude that the transient decline in lipoxygenase synthesis in excised soybean cotyledons is regulated at the RNA level. Similarly judged from the analysis of patterns of uni-dimensional gel electrophoresis, the synthesis of a few other polypeptides decreased during the first 4 h of culture as well, while several others increased; in cotyledons cultured for 20 to 40 h the protein-synthesis pattern had returned to that in freshly excised cotyledons. An acclimation period of ca 1 day seems to be needed for isolated soybean cotyledons to stabilize and to resume regular RNA and protein synthesis.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1399-3054.1988.tb06633.x
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  • 3
    Electronic Resource
    Electronic Resource
    Preface (1996)
    Oxford, UK : Blackwell Publishing Ltd
    Annals of the New York Academy of Sciences 792 (1996), S. 0 
    Details
    ISSN: 1749-6632
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Natural Sciences in General
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1749-6632.1996.tb32483.x
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  • 4
    Electronic Resource
    Electronic Resource
    Plant regeneration of wildGlycine species from suspension culture-derived protoplasts (1989)
    Springer
    Plant cell reports 8 (1989), S. 112-115 
    Details
    ISSN: 1432-203X
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Protoplasts isolated from four-week old cell suspension cultures ofGlycine canescens F. J. Herm andG. clandestina Wendl. were cultured in 8P or modified 8P to a multicellular stage. Colonies of 0.5 to 1.0 mm diameter were transferred to solid media for callus growth and regeneration. Callus consisted of friable masses with compact green nodular areas. Organogenesis of both species occurred primarily from the green nodular areas. Shoot buds ofG. clandestina did not mature, but shoots ofG. canescens proliferated on MS medium, with B5 vitamins, 0.33 mgL−1 each BA, KN, ZN, and 0.15 mgL−1 NAA. Shoots failed to root after multiple subcultures on four different rooting media.In vitro grafting ofG. canescens scions ontoG. max root stocks allowed plants to be transferred to soil. An overall protoplast division efficiency of 48% was achieved with moderately efficient shoot regeneration inG. canescens. Division efficiencies forG. clandestina were lower (11%). Refinements of this protocol should result in high efficiencies of regeneration which would allowin vitro manipulations of these wild soybean relatives at the single cell level and would make the derivation of somatic hybrid plants possible within the genusGlycine.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00716852
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  • 5
    Electronic Resource
    Electronic Resource
    Production of transgenic soybean lines expressing the bean pod mottle virus coat protein precursor gene (1996)
    Springer
    Plant cell reports 15 (1996), S. 746-750 
    Details
    ISSN: 1432-203X
    Keywords: Abbreviations: BAP: 6-benzyladenine phosphate; BPMV: bean pod mottle virus; CP-P: coat protein-precursor; CTAB: hexadecyltrimethylammonium bromide; DAS-ELISA: double antibody sandwich-enzyme-linked immunosorbent assay; IBA: indole-butyric acid; kbp: kilobase pairs; MES: 2-(N-Morpholino)ethanesulfonic acid; NOS: nopaline synthase; NPTII: neomycin phosphotransferase II; NTP: nucleoside triphosphate; PBS: phosphate-buffered saline; PCR: polymerase chain reaction; PVP: polyvinyl pyrrolidone; VPg: viral genome-linked protein.
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract. Soybean (Glycine max. Merrill. cv. Fayette) cotyledonary nodes were transformed with bean pod mottle virus (BPMV) coat protein precursor (CP-P) gene via Agrobacterium-mediated transformation. The transformation rate was low, and only five primary transformants derived from five different cotyledons were obtained from 400 original cotyledons. Southern blot hybridization verified the integration of the BPMV CP-P gene. Inheritance and expression of this gene in R1 plants were also demonstrated. About 30% of R2 plants derived from one transgenic line showed complete resistance to BPMV infection, as assessed by symptomatology and ELISA, suggesting that homozygous, but not hemizygous, plants exhibit the resistant phenotype.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s002990050112
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  • 6
    Electronic Resource
    Electronic Resource
    Genotypic and developmental regulation of transient expression of a reporter gene in soybean zygotic cotyledons (1994)
    Springer
    Plant cell reports 13 (1994), S. 556-560 
    Details
    ISSN: 1432-203X
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Processes involved in transformation of regenerable soybean (Glycine max (L.) Merrill) immature zygotic cotyledons were studied by assaying the transient expression of the β-glucuronidase gene driven by the 35S promoter and terminated at the 3′ end by the soybean 7S storage protein gene. The plasmid containing the chimeric gene was delivered to the cotyledons via particle bombardment 900 PSI. Zygotic cotyledons from six soybean varieties were tested for transient expression of the β-glucuronidase gene. The level of reporter gene expression differed between genotypes. The genotypes could be classified as high, fair or poor expressors. Cotyledons from different genotypes were then bombarded at 650, 900 or 1100 PSI. GUS expression varied among genotypes independently of the pressure of bombardment. Finally, the ability of cotyledons to express the reporter gene depended on the developmental stage of the seed from which it was excised with the younger stage being the least responsive. However, genotypic specific expression remained after controlling for developmental stage of the cotyledons.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00234510
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  • 7
    Electronic Resource
    Electronic Resource
    Production of transgenic soybean lines expressing the bean pod mottle virus coat protein precursor gene (1996)
    Springer
    Plant cell reports 15 (1996), S. 746-750 
    Details
    ISSN: 1432-203X
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Soybean (Glycine max. Merrill. cv. Fayette) cotyledonary nodes were transformed with bean pod mottle virus (BPMV) coat protein precursor (CP-P) gene via Agrobacterium-mediated transformation. The transformation rate was low, and only five primary transformants derived from five different cotyledons were obtained from 400 original cotyledons. Southern blot hybridization verified the integration of the BPMV CP-P gene. Inheritance and expression of this gene in R1 plants were also demonstrated. About 30% of R2 plants derived from one transgenic line showed complete resistance to BPMV infection, as assessed by symptomatology and ELISA, suggesting that homozygous, but not hemizygous, plants exhibit the resistant phenotype.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00232220
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  • 8
    Electronic Resource
    Electronic Resource
    Somatic embryogenesis and plant regeneration from zygotic embryo explants in mexican weeping bamboo, Otatea acuminata aztecorum (1992)
    Springer
    Plant cell reports 11 (1992), S. 257-261 
    Details
    ISSN: 1432-203X
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract An efficient protocol has been developed for the in vitro propagation of Mexican Weeping Bamboo through somatic embryogenesis from zygotic embryo explants. Mature seeds and excised embryos were cultured in the light or in the dark on both Murashige and Skoog's and Gamborg's B5 basal media with various supplements. Optimal somatic embryogenesis and plant regeneration were obtained by culture in the dark on Murashige and Skoog's basal medium supplemented with 3 mg/1 2,4-dichlorophenoxyacetic acid, 0.5 mg/1 6-benzylaminopurine and 2.0% sucrose. More than 95% of the germinating somatic embryos developed shoots and roots, and were transferred to soil with 85% success.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00235077
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  • 9
    Electronic Resource
    Electronic Resource
    Plant regeneration via somatic embryogenesis in pea (Pisum sativum L.) (1987)
    Springer
    Plant cell reports 6 (1987), S. 305-308 
    Details
    ISSN: 1432-203X
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Whole plant regeneration via somatic embryogenesis was obtained in pea (Pisum sativum L.) using explants from immature embryos or shoot apex segments. The induction of somatic embryos required picloram or 2,4-D. Germination of fully-developed embryos was accomplished by subculture on medium with only cytokinin and then on medium supplemented with cytokinins in combination with a reduced auxin concentration. Plantlets obtained from both zygotic embryos and shoot apices were transferred to soil and were grown to maturity. Nine plants were examined cytologically, revealing three tetraploids (2n=4x=28) and six diploids (2n=2x=14).
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00272005
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  • 10
    Electronic Resource
    Electronic Resource
    Expression of soybean-embryo lipoxygenase 2 in transgenic tobacco tissue (1992)
    Springer
    Planta 187 (1992), S. 203-208 
    Details
    ISSN: 1432-2048
    Keywords: Gene expression (lipoxygenase) ; Fatty acid peroxidation ; Lipoxygenase ; Lipid metabolism ; Nicotiana (transgenic)
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract To assess the role of lipoxygenase (LOX; EC 1.13.11.12) in plants, we increased the expression of LOX in the tissues of Nicotiana tabacum L. cv. ‘KY 14’ by over-expression of the LOX2 gene from the soybean (Glycine max (L.) Merrill) embryo. The LOX2 cDNA was manipulated by replacing its 5′-untranslated sequence with the translational enhancer of the alfalfa mosaic virus (AMV), and subcloned into a plant expression vector, 3′ to a duplicated cauliflower mosaic virus 35S promoter. The AMV-LOX2 construct was transferred into tobacco using Agrobacterium tumefaciens strain A281. The LOX2 was expressed in transgenic tobacco calli, leaves of transgenic plants, and their seed progeny at levels up to 0.1–0.2% of the total extracted protein. The introduced LOX2 affected fatty-acid oxidative metabolism as evidenced by a 50–529% increase in C6-aldehyde production. The impact on C6-aldehyde formation was greater than the effect on production of fatty-acid hydroperoxides. This is consistent with other studies indicating the greater propensity of soybean embryo LOX2 in generating C6-aldehydes than that of other well-characterized LOX isozymes.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00201939
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