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  • 1
    Electronic Resource
    Electronic Resource
    Functioning haemoglobin genes in non-nodulating plants (1988)
    [s.l.] : Nature Publishing Group
    Nature 331 (1988), S. 178-180 
    Details
    ISSN: 1476-4687
    Source: Nature Archives 1869 - 2009
    Topics: Biology , Chemistry and Pharmacology , Medicine , Natural Sciences in General , Physics
    Notes: [Auszug] We have searched for haemoglobin genes in a number of plant species. Using a soybean haemoglobin complementary DNA probe, we were unable to detect (because of sequence divergence) the haemoglobin gene sequence even in Parasponia, a nodulating species known to produce haemoglobin6. We were able to ...
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1038/331178a0
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  • 2
    Electronic Resource
    Electronic Resource
    Drought resistance in rice (1990)
    [s.l.] : Nature Publishing Group
    Nature 345 (1990), S. 26-27 
    Details
    ISSN: 1476-4687
    Source: Nature Archives 1869 - 2009
    Topics: Biology , Chemistry and Pharmacology , Medicine , Natural Sciences in General , Physics
    Notes: [Auszug] SIR-Drought is a major problem for much of the world's rice crop. Of the 146 million hectares of land on which rice is grown, only one half is irrigated. In the rest, which accounts for one-quarter of rice-grain production, yields are often depressed by drought12. Moreover, in many irrigated rice ...
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1038/345026b0
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  • 3
    Electronic Resource
    Electronic Resource
    Common evolutionary origin of legume and non-legume plant haemoglobins (1986)
    [s.l.] : Nature Publishing Group
    Nature 324 (1986), S. 166-168 
    Details
    ISSN: 1476-4687
    Source: Nature Archives 1869 - 2009
    Topics: Biology , Chemistry and Pharmacology , Medicine , Natural Sciences in General , Physics
    Notes: [Auszug] A number of plants outside the legume families are known to form nitrogen-fixing root nodules in symbiotic association with Rhizobium6 or actinomycetes7. Haemoglobin has been detected in nodules of several non-legume plants1'2 and characterized from Parasponia (Ulmaceae)8'9 and Casuarina ...
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1038/324166a0
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  • 4
    Electronic Resource
    Electronic Resource
    Expression of a bacterial gene in transgenic tobacco plants confers resistance to the herbicide 2,4-dichlorophenoxyacetic acid (1989)
    Springer
    Plant molecular biology 13 (1989), S. 533-540 
    Details
    ISSN: 1573-5028
    Keywords: 2,4-dichlorophenoxyacetic acid (2,4-D) ; detoxification ; herbicide resistance ; transgenic plants
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Plants resistant to the herbicide 2,4-dichlorophenoxyacetic acid (2,4-D) were produced through the genetic engineering of a novel detoxification pathway into the cells of a species normally sensitive to 2,4-D. We cloned the gene for 2,4-D monooxygenase, the first enzyme in the plasmid-encoded 2,4-D degradative pathway of the bacterium Alcaligenes eutrophus, into a cauliflower mosaic virus 35S promoter expression vector and introduced it into tobacco plants by Agrobacterium-mediated transformation. Transgenic tobacco plants expressing the highest levels of the monooxygenase enzyme exhibited increased tolerance to 2,4-D in leaf disc and seed germination assays, and young plants survived spraying with levels of herbicide up to eight times the usual field application rate. The introduction of the gene for 2,4-D monooxygenase into broad-leaved crop plants, such as cotton, should eventually allow 2,4-D to be used as an inexpensive post-emergence herbicide on economically important dicot crops.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00027313
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  • 5
    Electronic Resource
    Electronic Resource
    Functional properties of the anaerobic responsive element of the maize Adh1 gene (1990)
    Springer
    Plant molecular biology 15 (1990), S. 593-604 
    Details
    ISSN: 1573-5028
    Keywords: transcription ; promoter ; anaerobic stress ; anaerobic responsive element ; alcohol dehydrogenase
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The functional properties of the anaerobic responsive element (ARE) of the maize Adh1 gene have been analysed using a transient expression assay in electroporated maize protoplasts. The ARE functions in both orientations although inversion of the ARE sequence relative to the TATA box element produces slightly weaker promoter activity under anaerobic conditions and elevated expression under aerobic conditions. Promoter activity under anaerobic conditions is proportional to the number of complete ARE sequences in the Adh1 promotor. The ARE contains two sub-regions and dimers of sub-region II are as efficient as the wild-type sequence in activating gene expression under anaerobic conditions. However, sub-region I dimers do not appear capable of inducing gene expression in response to anaerobic stress. We conclude that sub-region II is essential for anaerobic induction of gene expression. Reporter gene expression remains constant when the spacing between sub-regions of the ARE is increased up to at least 64 bp, but increased spacing of 136 bp or greater abolishes expression in both aerobic and anaerobic conditions, indicating that a close association of the two sub-regions is required both for anaerobic responsiveness and for maximal levels of aerobic gene expression. When the ARE is placed upstream of position −90 of the CaMV 35S promoter, the ARE produces a high level of expression in both aerobic and anaerobic conditions. The general enhancement of gene expression driven by the hybrid ARE/35S promoter in aerobic conditions requires an intact sub-region II motif since mutation or deletion of sub-region II from the hybrid promoter reduces the level of expression to that observed for the truncated 35S promoter alone. In addition, mutation of the sub-region I sequences in the ARE/35S hybrid promoter does not significantly reduce expression in aerobic conditions, relative to pARE/Δ35S(-90), suggesting that sub-region I does not contribute to this general enhancer function.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00017834
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  • 6
    Electronic Resource
    Electronic Resource
    Characterization of pyruvate decarboxylase genes from rice (1996)
    Springer
    Plant molecular biology 31 (1996), S. 761-770 
    Details
    ISSN: 1573-5028
    Keywords: anoxia ; pyruvate decarboxylase ; rice ; submergence tolerance
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The pdc1 gene encoding pyruvate decarboxylase has been isolated and sequenced from an IR54 rice genomic library. In contrast to a previously isolated intron-less rice genomic pdc, pRgpdc3, this gene contains five intervening introns in the coding region and corresponds to a cDNA clone, pRcpdc1, isolated from an IR54-cDNA library constructed from anaerobically-induced mRNAs. Comparison of the deduced amino acid sequence of this gene with that of the rice pdc2 and pdc3 showed 88% and 89% similarity, and 78% and 79% identity, respectively. Southern blots indicated that more than three genes constitute the pdc gene family in rice. pdc1 is highly inducible under anaerobic conditions. Rice pdc2 is also inducible by anoxia but to a much lesser extent than pdc1.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00019464
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  • 7
    Electronic Resource
    Electronic Resource
    The maize transposable element Ac excises in progeny of transformed tobacco (1989)
    Springer
    Plant molecular biology 13 (1989), S. 109-118 
    Details
    ISSN: 1573-5028
    Keywords: Ac ; transposition ; progeny
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract To assess the potential of the maize transposable element Ac for gene tagging in heterologous plant species we monitored transcription, excision and transposition of the element in transgenic tobacco plants and their selfed progeny. Ac excised in the majority of primary regenerants and continued to excise in the first-generation progeny plants. In one primary regenerant Ac was transcribed but did not excise. Fourteen of eighteen kanamycin-resistant progeny from this plant showed Ac excision, suggesting that excision of Ac may have been activated during meiosis or in embryo development. This finding, together with the more general observation of continued Ac mobility in the progeny of transformed plants in which Ac had excised, suggests that Ac will be useful for gene tagging.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00027339
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  • 8
    Electronic Resource
    Electronic Resource
    Organ regulated expression of the Parasponia andersonii haemoglobin gene in transgenic tobacco plants (1988)
    Springer
    Molecular genetics and genomics 214 (1988), S. 68-73 
    Details
    ISSN: 1617-4623
    Keywords: Haemoglobin ; Nitrogen fixation ; Gene expression ; Plant transformation
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Plant haemoglobin genes are known to occur in legume and non-legume families and in both nodulating (e.g. Parasponia andersonii) and non-nodulating species (e.g. Trema tomentosa). Their presence in non-nodulating plants raises the possibility that haemoglobins might serve a function in non-symbiotic tissues distinct from their role in the nitrogen-fixing root nodules induced by micro-organisms. We report here that a P. andersonii haemoglobin promoter can regulate expression of either the P. andersonii haemoglobin gene, or a hybrid construct with the bacterial chloramphenicol acetyltransferase gene (cat), in the nonsymbiotic plant, Nicotiana tabacum. Expression is predominantly in the roots, implying that haemoglobins might have a function in roots of non-nodulated plants. We have also observed a low level of haemoglobin protein in non-nodulated P. andersonii roots, but not leaves, supporting this assertion. The expression in transgenic plants will allow further characterization of the promoter sequences essential for the organ-specific expression of haemoglobins in nonsymbiotic tissues.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00340181
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  • 9
    Electronic Resource
    Electronic Resource
    Molecular analysis of a somaclonal mutant of maize alcohol dehydrogenase (1986)
    Springer
    Molecular genetics and genomics 202 (1986), S. 235-239 
    Details
    ISSN: 1617-4623
    Keywords: Maize ; Alcohol dehydrogenase ; Somaclonal variation ; Mutation ; Gene sequence
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Plants regenerated from tissue cultures of maize were screened for variants of ADH1 and ADH2. Root extracts of 645 primary regenerant plants were tested, and one stable mutant of Adh1 was detected. The mutant gene (Adh1-Usv) produces a functional enzyme with a slower electrophoretic mobility than that of the progenitor Adh1-S allele, and is stably transmitted to progeny. The mutant was not present among four other plants derived from the same immature embryo, and therefore arose as a consequence of the culture procedure. The gene of Adh1-Usv was cloned and sequenced. A single base change in exon 6 was the only alteration found in the gene sequence. This would translate in the polypeptide sequence to a valine residue substituting for a glutamic acid residue, resulting in the loss of a negative charge and the production of a protein with slower electrophoretic mobility.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00331642
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  • 10
    Electronic Resource
    Electronic Resource
    Expression of the Talaromyces flavus glucose oxidase gene in cotton and tobacco reduces fungal infection, but is also phytotoxic (1999)
    Springer
    Molecular breeding 5 (1999), S. 219-232 
    Details
    ISSN: 1572-9788
    Keywords: disease resistance ; glucose oxidase ; Gossypium hirsutum ; Nicotiana tabacum ; Talaromyces flavus ; transgenic plant
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract Glucose oxidase secreted by the fungus Talaromyces flavus generates, in the presence of glucose, hydrogen peroxide that is toxic to phytopathogenic fungi responsible for economically important diseases in many crops. A glucose oxidase gene from T. flavus, was modified with a carrot extensin signal peptide and fused to either a constitutive or root-specific plant promoter. T1 tobacco plants expressing the enzyme constitutively were protected against infection by the seedling pathogen Rhizoctonia solani. Constitutive expression in tobacco was associated with reduced root growth, and slow germination on culture medium, and with reduced seed set in glasshouse conditions. Several independent transformed cotton plants with a root-specific construct expressed high glucose oxidase activity in the roots, excluding the root tip. Selected T3 homozygous lines showed some protection against the root pathogen, Verticillium dahliae, but not against Fusarium oxysporum. High levels of glucose oxidase expression in cotton roots were associated with reduced height, seed set and seedling germination and reduced lateral root formation. If this gene is to be of value for crop protection against pathogens it will require precise control of its expression to remove the deleterious phenotypes.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1023/A:1009625801909
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