ISSN:
1573-6881
Keywords:
Heart mitochondria
;
creatine phosphokinase
;
adenylate kinase
;
privileged access
;
mitochondrial inner membranes
;
mitoplasts
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
,
Chemistry and Pharmacology
,
Physics
Notes:
Abstract The concept that creatine phosphokinase is bound to the outer surface of the heart mitochondrial inner membrane originated from observations that the enzyme is retained by water-swollen heart mitochondria and by digitonintreated heart mitochondria suspended in isotonic sucrose. The present study establishes that digitonin-treated mitochondria release creatine phosphokinase in isotonic KCl, and other investigators have reported an identical response for the water-swollen organelles. These observations suggest that mitochondrial creatine phosphokinase is not bound to the outer surface of the inner membrane at a site adjacent to the adenine nucleotide translocase under physiologic conditions.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF00751106
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