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  • 1
    Online Resource
    Online Resource
    Singapore :Springer Singapore Pte. Limited,
    Keywords: Optical materials. ; Electronic books.
    Type of Medium: Online Resource
    Pages: 1 online resource (89 pages)
    Edition: 1st ed.
    ISBN: 9789811039478
    Series Statement: Springer Theses Series
    DDC: 621.3815
    Language: English
    Note: Intro -- Supervisor's Foreword -- Parts of this thesis have been published in the following journal articles: -- Acknowledgements -- Contents -- 1 Introduction -- 1.1 Background and Significance -- 1.2 Thin Films Composed of Assembled Ordered Nanowire Arrays -- 1.2.1 Interface-Induced Nanowire Assembly -- 1.2.1.1 Assembly of Nanowires by the Langmuir-Blodgett Technique -- 1.2.1.2 Evaporation-Induced Assembly of Nanowires -- 1.2.1.3 Other Nanowire Assemblies at Complex Interfaces -- 1.2.2 Nanowire Assemblies by Mechanical Force -- 1.2.3 Nanowire Assemblies Induced by External Nanostructures -- 1.2.4 Assembly of 1D Nanostructures by External Fields -- 1.2.4.1 Magnetic Field-Assisted Assembly of 1D Nanostructures -- 1.2.4.2 Electric Field-Assisted Assembly of 1D Nanostructures -- 1.2.5 Nanowire Assemblies by Microfluidic Flow -- 1.2.6 Nanowire Assemblies by Bubble Blowing Process -- 1.2.7 Nanowire Assemblies by Electrospinning -- References -- 2 Synthesis of One-Dimensional Te Nanostructures -- 2.1 Introduction -- 2.2 Microwave-Assisted Synthesis of Te Nanowires -- 2.3 Solution-Based Synthesis of Te Nanostructure -- References -- 3 Interface-Induced Macroscopic Nanowire Assemblies -- 3.1 Introduction -- 3.2 Interface-Induced Assembly of One-Dimensional Nanomaterials -- 3.2.1 Langmuir-Blodgett Technology for Assembly of Nanowires -- 3.2.1.1 LB Assembly of UltraFine and UltraLong Te Nanowires -- 3.2.1.2 Ordered Ultrathin Telluride Nanowires and Hetero-nanowire Film -- 3.2.1.3 Co-assembly of Ag and Te Nanowires -- 3.2.1.4 LB Assembly of Ultrathin W18O49 Nanowires -- 3.2.2 Assembly of Nanowires at Liquid-Liquid Interface -- 3.2.3 Shear Force-Induced Assembly of Silver and Tellurium Nanowires -- References -- 4 Electron-Beam-Induced Nanowire Assemblies -- 4.1 Introduction -- 4.2 Fabrication of Carbon Films by In Situ Electron-Beam Irradiation. , 4.3 Fabrication of Carbon-Based Nanocomposites by In Situ Electron-Beam Irradiation -- 4.4 Conclusions -- References -- 5 Applications of the Nanowire Assemblies -- 5.1 Introduction -- 5.2 Fabrication of Nanowire Stamper -- 5.3 Nanowire Selective Transmission -- 5.4 Nanowire Photoconductors -- 5.5 Nanowire Memory Device -- 5.6 Ordered Nanowire Films for Electrochromic Devices -- 5.7 Nanowire Films for Surface-Enhanced Raman Substrates -- 5.8 Flexible Transparent Electrodes Based on the Nanowire Assemblies -- References.
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  • 2
    Online Resource
    Online Resource
    San Diego :Elsevier,
    Keywords: Sulfide minerals. ; Electronic books.
    Type of Medium: Online Resource
    Pages: 1 online resource (248 pages)
    Edition: 1st ed.
    ISBN: 9780128198469
    DDC: 549/.32
    Language: English
    Note: Front Cover -- Fluid Inclusion Effect in Flotation of Sulfide Minerals -- Fluid Inclusion Effect in Flotation of Sulfide Minerals -- Copyright -- Contents -- 1 - Mineral fluid inclusions -- 1.1 Definition of mineral fluid inclusions -- 1.2 Formation and mechanism of inclusions -- 1.2.1 Growth and defects of mineral crystals -- 1.2.2 Fluid capture by fluid inclusion -- 1.2.2.1 Irregular crystal growth -- 1.2.2.2 Unbalanced growth of various parts inside the crystal -- 1.2.2.3 Inclusion formation through differences in the medium concentration -- 1.2.2.4 Discontinuity of crystal growth -- 1.2.2.5 Effects of solid-phase substances and impurities -- 1.2.2.6 Influence of temperature and pressure -- 1.3 Changes in fluid inclusion after fluid capture -- 1.3.1 Phase changes -- 1.3.1.1 Crystallization on the body wall -- 1.3.1.2 Daughter minerals -- 1.3.1.3 Shrinkage and immiscibility -- 1.3.1.4 Metastability -- 1.3.2 Physical changes -- 1.3.2.1 Volume and shape changes -- 1.3.2.2 Fluid infiltration and loss -- 1.4 Inevitability and universality of fluid inclusions -- Further reading -- 2 - Classification of fluid inclusions -- 2.1 Genetic classification of inclusions -- 2.1.1 Primary inclusions -- 2.1.2 Pseudo-secondary inclusions -- 2.1.3 Secondary inclusions -- 2.1.4 Metamorphosed inclusions -- 2.2 Classification of physical phase states of inclusions -- 2.2.1 Fluid inclusions -- 2.2.1.1 Pure liquid inclusions -- 2.2.1.2 Pure gas inclusions -- 2.2.1.3 Liquid inclusions -- 2.2.1.4 Gas inclusions -- 2.2.1.5 Daughter mineral inclusions -- 2.2.1.6 Carbon dioxide inclusions including liquid -- 2.2.1.7 Oil-gas inclusions -- 2.2.2 Magmatic inclusions -- 2.2.2.1 Crystalline melt inclusions -- 2.2.2.2 Vitreous melt inclusions -- 2.2.2.3 Fluid melt inclusions -- References -- 3 - Methods for the detection and composition study of fluid inclusions. , 3.1 General optical microscopy research on fluid inclusions -- 3.1.1 Preparation of inclusion sheets -- 3.1.1.1 General requirements for the production of double-sided polished sheets -- 3.1.1.2 Grinding process of double-sided polished sheets -- 3.1.2 Identification of fluid inclusions -- 3.1.3 Morphology of fluid inclusions under the microscope -- 3.1.3.1 Shape -- 3.1.3.2 Size -- 3.1.3.3 Distribution -- 3.1.3.4 Abundance -- 3.1.3.5 Color -- 3.1.4 Phase state of matter in fluid inclusions -- 3.1.4.1 Gaseous phase -- 3.1.4.2 Liquid phase -- 3.1.4.3 Solid phase -- 3.1.4.4 Hydrocarbon -- 3.2 Modern research techniques for identifying fluid inclusions -- 3.2.1 Ultraviolet light microscopy -- 3.2.2 Infrared microscopy -- 3.2.3 Scanning electron microscopy -- 3.2.4 High-resolution X-ray computed tomography -- 3.3 Determination of inclusions' salinity -- 3.3.1 Basic principle of determining salinity of inclusions by the freezing method -- 3.3.2 Notes on the determination of salinity in inclusions by the freezing method -- 3.3.3 Instruments for measuring the freezing point -- 3.4 Extraction and analysis of fluid inclusion components -- 3.4.1 Issues to be aware of in composition analysis of inclusions -- 3.4.2 Analysis of chemical composition of group inclusions -- 3.4.2.1 Extraction of inclusion component fluids -- 3.4.2.1.1 Individual mineral selection -- 3.4.2.1.2 Cleaning -- 3.4.2.1.3 Opening of inclusions -- 3.4.2.1.3.1 Mechanical crushing method -- 3.4.2.1.3.2 Grinding method (for mineral processing) -- 3.4.2.1.3.3 Thermal explosion method -- 3.4.2.1.4 Treatment before determination of liquid-phase component of inclusions -- 3.4.2.2 Component analysis methods for liquid and gas in inclusions -- 3.4.2.2.1 Inductively coupled plasma mass spectrometry -- 3.4.2.2.2 Ion chromatography method -- 3.4.2.2.3 Gas chromatography. , 3.4.2.2.4 Quadrupole mass spectrometry -- 3.4.3 Analysis of the chemical composition of individual inclusions -- 3.4.3.1 Destructive analysis of individual fluid inclusions -- 3.4.3.1.1 Laser ablation inductively coupled plasma mass spectrometry -- 3.4.3.1.2 Scanning electron microscopy/energy-dispersive X-ray spectroscopy analysis -- 3.4.3.1.3 Secondary ion mass spectrometry analysis -- 3.4.3.2 Nondestructive analysis of individual fluid inclusions -- 3.4.3.2.1 Laser Raman spectroscopy -- 3.4.3.2.2 Fourier transform Infrared -- 3.4.3.2.3 Synchrotron radiation X-ray fluorescence -- 3.4.3.2.4 Microbeam proton-induced X/γ-ray analysis -- References -- Further reading -- 4 - Internal composition of mineral fluid inclusions -- 4.1 Gaseous-phase composition of inclusions -- 4.2 Liquid-phase components of inclusions -- 4.3 Solid-phase composition of inclusions -- 4.4 Metal components in fluid inclusions -- 4.4.1 Analysis and calculation of heavy metal elements in ore-forming fluids -- 4.4.1.1 Sample selection -- 4.4.1.2 Sample preparation -- 4.4.1.3 Sample test -- 4.4.1.4 Dilution multiples and concentration conversion -- 4.4.2 Metal components in fluid inclusions in Cu-Au mineral -- 4.4.3 Metal components in fluid inclusions of Pb-Zn mineral -- 4.4.4 Metal components of group inclusions in quartz and pyrite -- References -- Further reading -- 5 - Component release of fluid inclusions in sulfide mineral -- 5.1 Analysis of mineral raw materials -- 5.1.1 Chalcopyrite and associated minerals -- 5.1.2 Sphalerite and quartz -- 5.1.3 Galena -- 5.1.4 Pyrite -- 5.1.4.1 Pyrite and quartz vein samples from the pyrite deposits in Weixin, Yunnan -- 5.1.4.2 Pyrite and quartz vein samples of polymetallic sulfide deposits from the Dapingzhang area of Yunnan -- 5.2 Research methods for fluid inclusions in sulfide mineral. , 5.3 Morphology and component release of fluid inclusions in chalcopyrite -- 5.3.1 Infrared optical microscopic analysis of the fluid inclusions in chalcopyrite -- 5.3.2 SEM/EDS of the position of fluid inclusions in chalcopyrite -- 5.3.3 HRXMT analysis -- 5.3.4 Component release of the fluid inclusions in chalcopyrite -- 5.4 Component release of the fluid inclusions in associated minerals of chalcopyrite -- 5.4.1 Component release of the fluid inclusions in the associated quartz and calcite of chalcopyrite -- 5.4.2 Component release of fluid inclusions in chalcopyrite, sphalerite, and associated minerals -- 5.5 Morphology and component release of fluid inclusions in sphalerite and associated quartz -- 5.5.1 Morphology and types of fluid inclusions in sphalerite and associated quartz -- 5.5.2 Freezing point and salinity value of the fluid inclusions in sphalerite -- 5.5.3 SEM/EDS analysis of the fluid inclusions on sphalerite surface -- 5.5.4 Component release of fluid inclusions in sphalerite and quartz -- 5.6 Morphology and component release of fluid inclusions in galena -- 5.6.1 Infrared optics and SEM/EDS of fluid inclusions in galena -- 5.6.2 Component release of inclusions in galena -- 5.7 Morphology and component release of fluid inclusions in pyrite -- 5.7.1 Infrared optical microscopic analysis of fluid inclusions of pyrite in Weixin -- 5.7.2 SEM/EDS detection of the positions of inclusions in pyrite from Weixin -- 5.7.3 High-resolution X-ray topography of microfault pyrite from Weixin -- 5.7.4 Component release of the fluid inclusions of pyrite from Weixin -- 5.7.5 Component release of quartz fluid inclusion in the pyrite deposits of Weixin -- 5.7.6 Component release of fluid inclusions of pyrite from the polymetallic sulfide deposits of Dapingzhang. , 5.7.7 Component release of the fluid inclusions of quartz from polymetallic sulfide deposits of Dapingzhang -- Further reading -- 6 - Solubility of sulfide mineral and chemical behaviors of solution after release of inclusion components -- 6.1 Solubility of sulfide mineral -- 6.1.1 Research methods -- 6.1.2 Solubility of the chalcopyrite surface -- 6.1.3 Solubility of the sphalerite surface -- 6.1.4 Solubility of the pyrite surface -- 6.2 Equilibrium theory calculation of solubility of sulfide minerals -- 6.2.1 Solubility of sulfide mineral in pure water -- 6.2.2 Solubility of sulfide mineral at different pH -- 6.3 Chemical equilibrium calculation of metal ions in slurry solution -- 6.3.1 Cu2+ taring and component distribution -- 6.3.2 Zn2+ taring and component distribution -- 6.3.3 Pb2+ taring and component distribution -- References -- 7 - Interactions among components of fluid inclusions in sulfide mineral, mineral surfaces, and collectors -- 7.1 ζ potential measurement of the adsorption of released components of inclusions on mineral surfaces -- 7.1.1 Test method for ζ potential -- 7.1.2 Surface adsorption of inclusion components in chalcopyrite -- 7.1.3 Surface adsorption of inclusion components in sphalerite -- 7.1.4 Surface adsorption of inclusion components in pyrite and galena -- 7.2 Density functional theory of the interactions between the components of fluid inclusion colonies and mineral surfaces -- 7.2.1 Crystal model and calculation method -- 7.2.1.1 Chalcopyrite crystal model and calculation method -- 7.2.1.2 Sphalerite crystal model and calculation method -- 7.2.1.3 Pyrite crystal model and calculation method -- 7.2.2 Surface relaxation and reconstitution of sulfide mineral -- 7.2.2.1 Surface structure of chalcopyrite -- 7.2.2.2 Surface structure of sphalerite -- 7.2.2.3 Surface structure of pyrite. , 7.2.3 Interaction of chalcopyrite surface and the copper in inclusions.
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  • 3
    Online Resource
    Online Resource
    Bristol :Institute of Physics Publishing,
    Keywords: Electronic books.
    Description / Table of Contents: Elliptical Mirrors: Applications in microscopy discusses the importance of the elliptical mirror; the third solution after parabolic reflectors and lenses for which apodization factors were established in 1921 and 1959 respectively. This detailed and highly insightful book will be an important reference in a growing subject area that will benefit PhD students, optical physicists, metrologists and researchers.
    Type of Medium: Online Resource
    Pages: 1 online resource (182 pages)
    Edition: 1st ed.
    ISBN: 9780750346603
    Series Statement: IOP Series in Advances in Optics, Photonics and Optoelectronics Series
    Language: English
    Note: Intro -- Series preface -- Preface -- Acknowledgement -- Editor biography -- Jian Liu -- List of contributors -- Chapter 1 Research and application of reflective microscopy -- 1.1 Introduction -- 1.2 Current situation of research on reflective microscopy -- 1.3 The current situation of application of reflective microscopy -- 1.4 Summary -- References -- Chapter 2 Apodization factor and linearly polarized light focusing properties of elliptical mirror -- 2.1 Introduction -- 2.2 Elliptical mirror model -- 2.3 Apodization factor -- 2.4 Apodization factor under different parametric variables -- 2.4.1 Apodization factor in terms of z -- 2.4.2 Apodization factor in terms of θ -- 2.5 Focusing properties based on the vector theory -- 2.5.1 Vector theories -- 2.5.2 Three-dimensional expression of the focused electric field -- 2.5.3 Numerical simulation of the focusing field -- 2.6 Comparison of focusing properties among elliptical mirror, parabolic mirror and lens -- 2.7 Summary -- References -- Chapter 3 Focusing characteristic of polarized light -- 3.1 Basic model of an elliptical mirror -- 3.2 Vector focus model of elliptical mirror with extra high aperture angle -- 3.2.1 Analysis of focusing characteristic of elliptical mirror under circularly polarized illumination -- 3.2.2 Analysis of focusing characteristics of the elliptical mirror under radially polarized illumination -- 3.3 Conclusion -- References -- Chapter 4 Imaging analysis of dipole vector in an elliptical mirror -- 4.1 Introduction -- 4.2 Imaging model of dipole vector in elliptical mirror -- 4.3 Imaging characteristics of the electric dipole in the elliptical mirror -- 4.4 Imaging characteristics of the electric dipole in a dual-lens system -- 4.5 Comparison on imaging characteristics of dipole in elliptical mirror, parabolic mirror and lens -- 4.6 Summary -- References. , Chapter 5 Scalar approximation for the focusing property of an elliptical mirror -- 5.1 Introduction -- 5.2 Influence factors of focusing property -- 5.2.1 Apodization factor -- 5.2.2 Polarization state -- 5.2.3 Wave aberration -- 5.3 Apodization factor of elliptical mirror -- 5.3.1 Apodization factor of thin lens -- 5.3.2 Apodization factor of the elliptical mirror with rotational symmetry -- 5.4 Analysis on focusing property of elliptical mirror -- 5.4.1 Focusing property of elliptical mirror with circular aperture -- 5.4.2 Focusing property of elliptical mirror with ring-shaped aperture -- 5.5 Comparative analysis on vector diffraction model -- 5.6 Summary -- References -- Chapter 6 Aberration analysis of an elliptical mirror with a high numerical aperture -- 6.1 Introduction -- 6.2 Analysis of geometrical aberration of elliptical mirror -- 6.2.1 Reflected ray formula of elliptical mirror -- 6.2.2 Analysis of the aberration coefficient of a single rotating elliptical mirror -- 6.3 Diffraction integral in the presence of aberration -- 6.3.1 Debye diffraction integral in the presence of aberration -- 6.3.2 Strehl intensity -- 6.4 Zernike circle polynomial expansion of aberration function -- 6.4.1 Transference theorem -- 6.4.2 Zernike circle polynomial -- 6.5 Primary aberration and its influence on the focusing characteristic of the elliptical mirror -- 6.5.1 Primary spherical aberration -- 6.5.2 Primary coma -- 6.5.3 Primary astigmatism -- 6.5.4 Field curvature and distortion -- 6.5.5 Aberration tolerance of elliptical mirror -- 6.6 Conclusion -- References -- Chapter 7 Three-dimensional transfer function -- 7.1 Introduction -- 7.2 Point spread function -- 7.2.1 Coherent transfer function -- 7.2.2 Optical transfer function of elliptical mirror -- 7.3 Three-dimensional transfer function of an elliptical reflective confocal microscopic system. , 7.3.1 Coherent transfer function of elliptical reflective confocal microscopic system -- 7.3.2 Two-dimensional transfer function of the elliptical reflective confocal microscopic imaging system -- 7.4 Summary -- References -- Chapter 8 Design and application of an aspherical mirror -- 8.1 Introduction -- 8.2 Basic knowledge -- 8.2.1 Mathematical representation of aspherical surface -- 8.2.2 Taylor series -- 8.3 Design of reflective objective -- 8.3.1 Head design model -- 8.3.2 Aperture diaphragm and field diaphragm -- 8.4 Decoupled model based on the Taylor series expansion -- 8.4.1 Taylor series expansion of the Head polar coordinate model -- 8.4.2 Taylor series expansion of a quadric surface -- 8.4.3 Determination of reflective objective parameters -- 8.4.4 Derivation and truncation error of a high-order aspherical surface parameter -- 8.4.5 Effect of numerical aperture on a decoupled model -- 8.5 Design method based on an obscuration constraint -- 8.5.1 Analysis of the obscuration effect on a reflective objective -- 8.5.2 Obscuration constraint model -- 8.5.3 Design method based on obscuration constraint -- 8.6 Industrial application -- 8.6.1 Structural parameter calculation -- 8.6.2 Obscuration verification -- 8.6.3 Analysis of initial configuration imaging characteristics -- 8.6.4 Whole equipment and specific application -- 8.7 Summary -- References -- Chapter 9 Elliptical mirror applied in TIRF microscopy -- 9.1 Introduction -- 9.2 Background -- 9.3 Basic theory -- 9.4 Experiments -- 9.5 Summary -- References.
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  • 4
    Keywords: Forschungsbericht
    Type of Medium: Online Resource
    Pages: Online-Ressource (527 S., 22,3 MB) , Ill., graph. Darst., Kt.
    Language: English
    Note: Förderkennzeichen BMBF 0330799A. - Verbund-Nr. 01055052. - Literaturangaben , Unterschiede zwischen dem gedruckten Dokument und der elektronischen Ressource können nicht ausgeschlossen werden , Auch als gedr. Ausg. vorhanden , Systemvoraussetzungen: Acrobat reader. , Text in engl.
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  • 5
    Online Resource
    Online Resource
    San Rafael :Morgan & Claypool Publishers,
    Keywords: Confocal microscopy. ; Electronic books.
    Type of Medium: Online Resource
    Pages: 1 online resource (90 pages)
    Edition: 1st ed.
    ISBN: 9781681743370
    Series Statement: IOP Concise Physics Series
    DDC: 681.413
    Language: English
    Note: Intro -- Preface -- Acknowledgments -- Author biography -- Jian Liu -- Chapter 1 Confocal microscopy and its application in China -- 1.1 A brief review of confocal microscopy -- 1.2 Resolution -- 1.3 Standardization in China -- References -- Chapter 2 Point spread function model -- 2.1 Lens imaging -- 2.2 PSF in confocal imaging -- References -- Chapter 3 Incoherent three-dimensional optical transfer function -- 3.1 Development of 3D optical transfer function -- 3.2 3D imaging models of CM -- 3.3 3D-OTF of CM -- 3.4 3D-OTF of differential CM -- References -- Chapter 4 Decoupling criteria for three-dimensional optical microscopic measurement -- 4.1 Introduction -- 4.2 Decoupling model for the measurement of thin samples -- 4.3 Decoupling model for the measurement of a deep groove sample -- 4.4 Experiments -- References -- Chapter 5 Pupil filter design -- 5.1 Phase rotation transformation -- 5.2 The design method of filters with global minimizing side lobes -- References -- Chapter 6 Confocal axial peak extraction algorithm -- 6.1 Introduction -- 6.2 Centroid method for localization of confocal peak -- 6.3 Nonlinear fitting method for peak localization -- 6.4 Deviation analysis for localization of confocal axial peak -- References -- Chapter 7 Differential confocal microscopy -- 7.1 Introduction -- 7.2 Application of DCM in China -- 7.3 The Basic principle of DDCM -- References -- Chapter 8 Medium aided scattering measurement -- 8.1 Introduction -- 8.2 The principle of medium aided scattering confocal microscopy -- 8.3 Analysis of deposition uniformity of a fluorescent medium layer -- 8.4 Error analysis and height correction of the medium layer -- 8.5 Application of medium aided scattering confocal microscopy -- References -- Chapter 9 Scanning technology -- 9.1 Introduction -- 9.2 Scanners -- 9.3 Raster scanning -- 9.4 α-β circular scanning. , References -- Chapter 10 Confocal profilometer -- 10.1 Introduction -- 10.2 Basic principle -- 10.3 The extraction method of discrete surface -- 10.3.1 Method of planar substrate conversion -- 10.3.2 Method of polynomial regression -- 10.4 Application of confocal profilometer -- References.
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  • 6
    ISSN: 1520-4995
    Source: ACS Legacy Archives
    Topics: Biology , Chemistry and Pharmacology
    Type of Medium: Electronic Resource
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  • 7
    Electronic Resource
    Electronic Resource
    s.l. : American Chemical Society
    Macromolecules 25 (1992), S. 7365-7372 
    ISSN: 1520-5835
    Source: ACS Legacy Archives
    Topics: Chemistry and Pharmacology , Physics
    Type of Medium: Electronic Resource
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  • 8
    ISSN: 1520-5126
    Source: ACS Legacy Archives
    Topics: Chemistry and Pharmacology
    Type of Medium: Electronic Resource
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  • 9
    ISSN: 1520-5126
    Source: ACS Legacy Archives
    Topics: Chemistry and Pharmacology
    Type of Medium: Electronic Resource
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  • 10
    ISSN: 1432-0533
    Keywords: Key words Advanced glycation endproducts ; Familial amyotrophic lateral sclerosis ; G85R transgenic mice ; Granule-coated fibrils ; Superoxide dismutase-1
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract To clarify the biological significance of the neuronal Lewy body-like hyaline inclusions and astrocytic hyaline inclusions characteristically found in patients with familial amyotrophic lateral sclerosis with superoxide dismutase-1 (SOD1) gene mutations and in transgenic mice expressing human SOD1 with G85R mutation, the detailed protein composition in both types of inclusions was immunohistochemically analyzed using 45 different antibodies. Both types of inclusions had very strong immunoreactivity for SOD1. The SOD1-positive inclusions in both cell types were also immunoreactive for the insoluble advanced glycation endproducts (AGEs) such as N ɛ-(carboxymethyl)lysine (CML), pyrraline and pentosidine: both inclusions in both conditions were ultrastructurally composed of the granule-coated fibrils that had immunoreactivities to CML and pyrraline. Both types of inclusions were negative for stress-response proteins (SRPs), 4-hydroxy-2-nonenal (HNE), acrolein, nitric oxide synthases (NOSs) and nitrotyrosine as representative markers of oxidative stress. The neurons and astrocytes of the normal individuals and non-transgenic mice showed no significant immunoreactivity for SOD1, AGEs, SRPs, HNE, acrolein, NOSs or nitrotyrosine. Our results suggest that a portion of the SOD1 composing both type of inclusions, probably toxic mutant SOD1, is modified by the AGEs, and that the formation of the AGE-modified SOD1 is one of the mechanisms responsible for the aggregation involving no significant oxidative mechanisms.
    Type of Medium: Electronic Resource
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