Description: The Southern Ocean is an important biogeochemical region on a global scale, in which mineralising phytoplankton play a role in cycling energy, carbon and nutrients. Mineralising phytoplankton with cells 2-20. μm in diameter (nannoplankton) are poorly enumerated by traditional preservation and microscopy techniques, yet may fulfil an important role in the Southern Ocean. Here we define the spatial and temporal biogeography for these mineralising nannoplankton assessed by scanning electron microscopy in conjunction with an array of biological, physical, and chemical variables during two cruises to the Scotia Sea region of the Southern Ocean. The cruises encompassed two seasons, austral summer (January-February 2008) and austral autumn (March-April 2009).The biogeography of the three most numerous mineralising nannoplankton groups, the coccolithophore Emiliania huxleyi, the smaller (〈10μm) species of the diatom genus Fragilariopsis, and chrysophytes of the genus Tetraparma (mostly Tetraparma pelagica) were found to be related to the boundaries of the major circumpolar fronts. E. huxleyi abundances were relatively high in the northern water masses (maximum of 650cellsml -1), while T. pelagica abundances were high in the southern water masses (maximum of 1910cellsml -1). Small Fragilariopsis spp. abundances were also highest in the southern water masses (maximum of 1820cellsml -1), but this group was present throughout the Scotia Sea.Multivariate statistical analysis found that the most influential environmental variables controlling mineralising nannoplankton biogeography were sea surface temperature and silicate concentration. Estimates of biomass indicated that the Scotia Sea mineralising nannoplankton community formed a substantial part of the total phytoplankton community, particularly south of the Southern Antarctic Circumpolar Current Front (SACCF) during the austral autumn, where mineralising nannoplankton biomass reached 36 of the total phytoplankton biomass. The results that are obtained suggest that traditional microscopic surveys of large Southern Ocean phytoplankton may underestimate total biomass by excluding key mineralising nannoplankton groups. Greater appreciation of the ecological significance of mineralising nannoplankton in the Southern Ocean will improve our understanding of the relationships between environmental parameters, primary production, and the biological carbon pump in this ecosystem. © 2011 Elsevier Ltd.

Description: The Southern Ocean is largely a High Nutrient Low Chlorophyll (HNLC) region where macronutrient concentrations are high and phytoplankton productivity is low. However, there are productive 'hot spots' that sustain large phytoplankton blooms. These areas, maintained by natural iron (Fe) fertilization, are important for the Southern Ocean ecosystem and for driving carbon export. Fe addition on-deck bioassay experiments were conducted on two cruises to the Scotia Sea region of the Southern Ocean (austral spring 2006 and summer 2008) to better understand how Fe controls the microplankton (20-200μm) community structure on a seasonal basis. Light microscopy and fast-repetition rate fluorometry were used to examine changes in the species composition and physiological status of the microplankton community. Bioassays were carried out in three contrasting regions of the Scotia Sea: (1) a naturally Fe-fertilized, high chlorophyll area downstream (north and northwest) of the Islands of South Georgia (DSG); (2) a low Fe, low chlorophyll area upstream (south) of the Islands of South Georgia (USG); and (3) a naturally Fe-fertilized area north of the South Orkney Islands (SOI). Multivariate statistics were applied to the light microscopy results, showing significant differences between the initial microplankton communities for each of the bioassays. These differences were primarily spatial (between regions) and secondarily temporal (between seasons). Significant microplankton community shifts occurred in three of five bioassays, those in spring and summer USG and in summer DSG only. In summer, USG community responses increased significantly in medium (100-1000pgCcell -1) and large (>1000pgCcell -1) diatom species in response to Fe addition. Such a response was consistent with relief from in situ Fe limitation, which favours larger microplankton species with higher Fe requirements and subject to lower grazing pressures. The largest biomass increase in Fe-treated bioassay bottles was in Pseudonitzschia spp., which suggests that this genus may be a particularly important member of the microplankton community in the Scotia Sea. © 2011 Elsevier Ltd.