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  • 1
    Electronic Resource
    Electronic Resource
    Cloning vectors for Streptococcus thermophilus derived from a native plasmid (2002)
    Oxford, UK : Blackwell Publishing Ltd
    FEMS microbiology letters 216 (2002), S. 0 
    Details
    ISSN: 1574-6968
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Notes: A 3.5-kb native plasmid (pND103) was identified in Streptococcus thermophilus ST2-1. Preliminary sequence analysis indicated that pND103 belongs to group I S. thermophilus plasmids. A region of approximately 2 kb appears to contain three components: a plus origin of replication (ori) typical of plasmids that replicate via rolling circle replication; a gene encoding a replication protein (rep); and a gene encoding a small heat shock protein (hsp). pND103 was then used to construct S. thermophilus/Escherichia coli hybrid cloning vectors by ligating different portions of pND103 to an origin-probe vector (pND330) composed of pUC19 and a Gram-positive erythromycin resistance gene. The shuttle vectors (pND913, pND914 and pND915) were successfully introduced back into plasmid-free S. thermophilus ST3–1 as well as to Lactococcus lactis LM0230 and E. coli JM109. Segregational and structural stability study indicated that these vectors can be maintained in these hosts. The results indicated that pND913, pND914 and pND915 are potential shuttle cloning vectors for S. thermophilus.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1574-6968.2002.tb11412.x
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  • 2
    Electronic Resource
    Electronic Resource
    The Lactococcus lactis sex-factor aggregation gene cluA (1994)
    Oxford, UK : Blackwell Publishing Ltd
    Molecular microbiology 12 (1994), S. 0 
    Details
    ISSN: 1365-2958
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology , Medicine
    Notes: A gene, cluA, was cloned from the chromosomally located sex factor of Lactococcus lactis MG1363. Sequence analysis revealed significant homology with previously described aggregation proteins in Enterococcus and Streptococcus species. The possibility that cluA was an equivalent protein involved in cell aggregation between donor and recipient bacteria during lactococcal conjugation was confirmed by its expression under the control of a heterologous promoter in L. lactis. Analysis of the homology between the CluA protein and the related proteins of Enterococcus and Streptococcus allowed a common structure for these proteins to be postulated. This consisted of five domains. Functionally conserved domains I and V act respectively as a secretary leader and C-terminal membrane anchor. Domains II and IV are conserved at the amino acid level and probably have common structural roles whereas domain III is variable and may control binding specificity.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1365-2958.1994.tb01053.x
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  • 3
    Electronic Resource
    Electronic Resource
    Autolytic Lactococcus Lactis Expressing a Lactococcal Bacteriophage Lysin Gene (1992)
    [s.l.] : Nature Publishing Company
    Nature biotechnology 10 (1992), S. 196-199 
    Details
    ISSN: 1546-1696
    Source: Nature Archives 1869 - 2009
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: [Auszug] We report the expression of a cloned lysin gene of bacteriophase ØvML3 in dairy starter strains of the species Lactococcus lactis subsp. lactis and L. lactis subsp. cremoris. Lactococcal strains, which are sensitive to lysin, were unaffected by its expression during exponential growth but ...
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1038/nbt0292-196
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  • 4
    Electronic Resource
    Electronic Resource
    Cloning and DNA sequence analysis of a Lactococcus bacteriophage lysin gene (1989)
    Springer
    Molecular genetics and genomics 218 (1989), S. 214-221 
    Details
    ISSN: 1617-4623
    Keywords: Bacteriophage ; Lysin ; Lactococcus ; Lactic streptococci ; Dairy starter cultures
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary A gene for the lysin of Lactococcus lactis bacteriphage ΦvML3 was cloned using an Escherichia coli/bacteriophage lambda host-vector system. The gene was detected by its expression of antimicrobial activity against L. lactis cells in a bioassay. The cloned fragment was analysed by sub-cloning on to E. coli plasmid vectors and by restriction endonuclease and deletion mapping. Its entire DNA sequence was determined and an open reading frame for the lysin structural gene was identified. The sequenced lysin gene would express a protein of 187 amino acids with a molecular weight of 21090, which is in good agreement with that of a protein detected after in vitro transcription and translation of DNA encoding the gene. Expression of the lysin gene in E. coli and B. subtilis from an adjacent bacteriophage promoter was readily detected but in L. lactis expression of lysin was found to be lethal. The bacteriophage ΦvML3 lysin had sequence homology with protein 15 of B. subtilis bacteriophage PZA. This protein is involved in DNA packaging during bacteriophage muturation rather than in host cell lysin. The cloning and analysis of the ΦvML3 lysin gene is of importance in further understanding lactic streptococcal bacteriophages, for the development of positive selection vectors and for biotechnological applications of relevance to the dairy industry.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00331271
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  • 5
    Electronic Resource
    Electronic Resource
    Improvement of plasmid encoded lactococcal bacteriophage resistance by mutator strain Epicurian coli XL1-Red (2000)
    Springer
    Biotechnology letters 22 (2000), S. 721-725 
    Details
    ISSN: 1573-6776
    Keywords: abortive infection ; bacteriophage resistance ; mutator strain ; random mutagenesis ; XL1-Red
    Source: Springer Online Journal Archives 1860-2000
    Topics: Process Engineering, Biotechnology, Nutrition Technology
    Notes: Abstract A random mutation strategy using mutator strain, Epicurian coli XL1-Red, was applied to a plasmid, pND018, constructed by inserting a Lactococcus lacis bacteriophage resistance gene (abiI) into a L. lactis/E. coli shuttle vector (pDL278), to introduce random mutations throughout the plasmid. Following transformation of the mutated plasmid library to a plasmid free and phage sensitive strain of L. lactis (LM0230), mutated plasmids were screened by cross-streaking and efficiency of plaquing (EOP) assays. Two strains with enhanced resistance were obtained, as well as several phage sensitive strains. Repeated transformation of the mutated plasmids to LM0230 confirmed that the observed phenotypes were caused by mutations located on the plasmids. The EOP values and plaque morphology of two enhanced phage resistance mutants were characterized at 30 °C and 37 °C. These results indicate that this simple procedure can be applied to generate modified plasmids with improved phage resistance, which may be of commercial value.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1023/A:1005672109400
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