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  • 1
    Digitale Medien
    Digitale Medien
    D-cycloserine biases enrichment for auxotrophic mutants of Zymomonas mobilis (1996)
    Oxford, UK : Blackwell Publishing Ltd
    FEMS microbiology letters 142 (1996), S. 0 
    Details
    ISSN: 1574-6968
    Quelle: Blackwell Publishing Journal Backfiles 1879-2005
    Thema: Biologie
    Notizen: Abstract Contrary to its effect on rich medium, d-cycloserine showed no bactericidal effect on Zymomonas mobilis cells cultured on mineral medium. Addition of a mixture of glycine and glutamic acid to the mineral medium restored its bactericidal action. However, mutant enrichments run in these conditions were biased, with mostly methionine mutants isolated. A decrease of the d-cycloserine concentration only reduced the bias.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1111/j.1574-6968.1996.tb08425.x
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  • 2
    Digitale Medien
    Digitale Medien
    Salt-induced photosystem I cyclic electron transfer restores growth on low inorganic carbon in a type 1 NAD(P)H dehydrogenase deficient mutant of Synechocystis PCC6803 (1998)
    Oxford, UK : Blackwell Publishing Ltd
    FEMS microbiology letters 167 (1998), S. 0 
    Details
    ISSN: 1574-6968
    Quelle: Blackwell Publishing Journal Backfiles 1879-2005
    Thema: Biologie
    Notizen: The cyanobacterium Synechocystis PCC6803 induces a photosystem I cyclic electron transfer route independent of type 1 NAD(P)H dehydrogenase. The capacity to tolerate raised salinity conditions was shown to operate in a mutant lacking functional type 1 NAD(P)H dehydrogenase. The mutant showed salt-induced enhancement of photosystem I cyclic electron transfer and respiratory capacities. Moreover, this salt-adapted energetic state also restored the capacity of the mutant to grow under inorganic carbon limitation. Uptake of the latter in these conditions became almost as efficient as in the wild-type. The acquired energetic capacities, in contrast, did not allow restoration of photoheterotrophic growth in the type 1 NAD(P)H dehydrogenase mutant.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1111/j.1574-6968.1998.tb13218.x
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  • 3
    Digitale Medien
    Digitale Medien
    Obligate phototrophy in cyanobacteria: more than a lack of sugar transport (1998)
    Oxford, UK : Blackwell Publishing Ltd
    FEMS microbiology letters 161 (1998), S. 0 
    Details
    ISSN: 1574-6968
    Quelle: Blackwell Publishing Journal Backfiles 1879-2005
    Thema: Biologie
    Notizen: DNA hybridization using the Synechocystis PCC6803 glucose transporter gene, glcP, revealed a single homologous region in two facultative photoautotrophic strains out of three tested, and none in three obligate autotrophs. In one of the latter, Synechococcus PCC7942, integration of glcP into the chromosome resulted in glucose sensitivity. A subclone isolated as glucose-tolerant had lost glcP. Integration in a replicative vector allowed glucose transport and photoheterotrophic growth, but could not be maintained. Thus lack of sugar transport could explain cyanobacterial obligate autotrophy. However, at least in Synechococcus PCC7942, acquisition of such a transport capacity created a metabolic disequilibrium barely compatible with survival.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1111/j.1574-6968.1998.tb12959.x
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  • 4
    Digitale Medien
    Digitale Medien
    Variations of protein profiles upon shifts in inorganic carbon regime in the cyanobacterium Synechocystis PCC6803 (1998)
    Oxford, UK : Blackwell Publishing Ltd
    FEMS microbiology letters 164 (1998), S. 0 
    Details
    ISSN: 1574-6968
    Quelle: Blackwell Publishing Journal Backfiles 1879-2005
    Thema: Biologie
    Notizen: Reversible changes between limiting and high inorganic carbon regimes in Synechocystis PCC6803 maintained overall protein synthesis at a high level. The protein patterns of cells during or after adaptation to these regimes were detected by pulse labelling and identification after one-dimensional and two-dimensional electrophoreses. Several particular patterns were observed. Proteins specific for either the high or the limiting carbon conditions could be identified. The protein profiles of cells having reached the stationary phase of growth clearly differed from those of their exponentially growing counterparts. Accumulation of Rubisco, the key enzyme of the Calvin cycle, was not modified in the conditions tested.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1111/j.1574-6968.1998.tb13083.x
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  • 5
    Digitale Medien
    Digitale Medien
    Flavodoxin accumulation contributes to enhanced cyclic electron flow around photosystem I in salt-stressed cells of Synechocystis sp. strain PCC 6803 (1999)
    Copenhagen : Munksgaard International Publishers
    Physiologia plantarum 105 (1999), S. 0 
    Details
    ISSN: 1399-3054
    Quelle: Blackwell Publishing Journal Backfiles 1879-2005
    Thema: Biologie
    Notizen: The salt-regulated accumulation of flavodoxin encoded by the isiB gene and its possible function were investigated in the cyanobacterium Synechocystis sp. strain PCC 6803. In Northern blot experiments, a slight increase of the isiB-specific mRNA was observed in salt-shocked and salt-acclimated cells. High levels of flavodoxin protein were detected in cells acclimated to 342 mM NaCl. In order to analyze the function of flavodoxin in cyanobacterial salt acclimation, an insertion null mutant of isiB was constructed. It was possible to adapt this mutant to raised salt concentrations and, as expected, to low iron contents. Salt-acclimated cells of wild type (WT) Synechocystis display increased activity of photosystem I (PSI), primarily used for increased cyclic electron transport capacity (Jeanjean et al. 1993, Plant Cell Physiol 34: 1073–1079). In salt-acclimated cells of the flavodoxin null mutant, the level of cyclic electron flow was lower than in wild type cells. It was concluded that flavodoxin plays a role as an alternative electron carrier, used for cyclic electron flow in salt-treated Synechocystis cells.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1034/j.1399-3054.1999.105411.x
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  • 6
    Digitale Medien
    Digitale Medien
    Dynamics of the response of cyanobacteria to salt stress: Deciphering the molecular events (1996)
    Oxford, UK : Blackwell Publishing Ltd
    Physiologia plantarum 96 (1996), S. 0 
    Details
    ISSN: 1399-3054
    Quelle: Blackwell Publishing Journal Backfiles 1879-2005
    Thema: Biologie
    Notizen: Cyanobacteria, the only prokaryotes performing oxygemc photosynthesis and probable ancestors of chloroplasts, constitute valuable models for the study of the molecular mechanisms involved in tolerance to high salinity, or to its corollary, drought, a major agricultural problem. The critical demands of cyanobacteria exposed to high salinity, i.e., accumulation of osmoprotectors and extrusion of sodium ions, are met through immediate activation and/or long term (protein synthesis-dependent) adaptation of various processes: (1) uptake and endogenous biosynthesis of osmotica, the nature and amount of which are strain- and salt concentration-dependent; (2) enhancement of P-ATPase activity and active extrusion of sodium ions; (3) probable modifications of membrane lipid composition: and (4) increased energetic capacity, at the level of cyclic electron flow around photosystem I (through routes induced under these conditions) and cytochrome c oxidase. The processes involved highlight similarities with general stress responses and with salt stress responses in plants. Deciphering the molecular and genetic events regulating these coordinated responses is presently starting in cyanobacteria.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1111/j.1399-3054.1996.tb00251.x
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  • 7
    Digitale Medien
    Digitale Medien
    Sulphated exopolysaccharides produced by two unicellular strains of cyanobacteria, Synechocystis PCC 6803 and 6714 (1988)
    Springer
    Archives of microbiology 150 (1988), S. 558-563 
    Details
    ISSN: 1432-072X
    Schlagwort(e): Cyanobacteria ; Synechocystis sp. ; Carbohydrates ; Exopolysaccharides ; Sulphated sugars
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie
    Notizen: Abstract The exopolysaccharides (EPS) of two unicellular strains of cyanobacteria Synechocystis PCC 6803 and 6714, formed labile, radial structures, uniformly distributed on the cell surface, and stainable by specific dyes for acidic polysaccharides. The two strains produced EPS at similar rates, which depended, along with the duration of the producing phase, on the incubation conditions. The exopolysaccharides from both strains were constituted of at least 11–12 mono-oses, probably forming several types of polymers. They contained about 15–20% (w/w) uronic derivatives and 10–15% (w/w) osamines. Proteins represented 20–40% of total weight. A most interesting feature was the presence of 7–8% (molar ratio) sulphate residues, a characteristic that is otherwise limited to exopolysaccharides produced by eukaryotic algae.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF00408249
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  • 8
    Digitale Medien
    Digitale Medien
    A protein involved in co-ordinated regulation of inorganic carbon and glucose metabolism in the facultative photoautotrophic cyanobacteriumSynechocystis PCC6803 (1994)
    Springer
    Plant molecular biology 25 (1994), S. 855-864 
    Details
    ISSN: 1573-5028
    Schlagwort(e): cyanobacteria ; intermediary metabolism ; Calvin cycle ; trophic adaptation
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie
    Notizen: Abstract The involvement of a gene ofSynechocystis PCC6803,icfG, in the co-ordinated regulation of inorganic carbon and glucose metabolism, was established. TheicfG gene codes for a 72 kDa protein, which shows no homology with those registered in data libraries. Expression oficfG required glucose, the actual inducer probably being glucose-6-phosphate, and was independent of light and of the external inorganic carbon concentration. Mutants carrying an inactivated copy oficfG were constructed. Their growth characteristics were identical to those of the wild type under all regimes except in limiting inorganic carbon with glucose being present either before or after the transfer to the limiting conditions. These conditions completely prevented growth, both in the light and in the dark. The inhibition could be relieved by several intermediates of the tricarboxylic acid cycle. Assays of various enzymic activities related to inorganic carbon uptake and to its assimilationvia either the Calvin cycle or phosphoenolpyruvate carboxylase did not reveal the level of action of IcfG. Possible models include a blockage of the assimilation of both carbon sources in the absence of IcfG, or the inhibition of Ci incorporation route(s) essential under limiting inorganic carbon conditions, even when glucose is present, and even in the dark.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF00028880
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  • 9
    Digitale Medien
    Digitale Medien
    A protein is involved in accessibility of the inhibitor acetazolamide to the carbonic anhydrase(s) in the cyanobacterium Synechocystis PCC 6803 (1995)
    Springer
    Plant molecular biology 27 (1995), S. 779-788 
    Details
    ISSN: 1573-5028
    Schlagwort(e): cyanobacteria ; inorganic carbon ; carbonic anhydrase ; acetazolamide ; resistant mutant ; nucleotide sequence
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie
    Notizen: Abstract A gene, zam (for resistance to acetazolamide), controlling resistance to the carbonic anhydrase inhibitor acetazolamide, is described. It has been cloned from a spontaneous mutant, AZAr-5b, isolated from the cyanobacterium Synechocystis PCC 6803, for its resistance to this drug (Bédu et al., Plant Physiol 93: 1312–1315, 1990). This mutant, besides its resistance to acetazolamide, displayed an absence of catalysed oxygen exchange activity on whole cells, suggestive of a deficiency in carbonic anhydrase activity. The gene was isolated by screening a genomic library of AZAr-5b, and selecting for the capacity to transfer the AZAr phenotype to wild-type cells. A system leading to forced homologous recombination in the host chromosome, using a platform vector, was devised in order to bypass direct selection difficulties. The putative encoded protein, 782 amino acids long, showed some homology with four eukaryotic and prokaryotic proteins involved in different cellular processes, one of them suppressing a phosphatase deficiency. The mutated allele of AZAr-5b showed an in-frame 12 nucleotide duplication, which should not interfere with translation, and might result from transposition of a mobile element. Integration into a wild-type genome of either the spontaneous mutated allele or one inactivated by insertional mutagenesis conferred the character of resistance, but not the deficiency in oxygen exchange, indicating that the two phenotypic aspects of AZAr-5b corresponded to two independent mutations. A working hypothesis explaining the phenotypes of the mutants is that the presence of the Zam protein would be necessary for the inhibitor to reach (one of) the two carbonic anhydrases present in this strain. This, however, would be a secondary action, the physiological role of the protein still being cryptic.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF00020230
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