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  • 1
    Digitale Medien
    Digitale Medien
    Measurements of the Growth and Distribution of Mammalian Cells in a Hollow–Fiber Bioreactor Using Nuclear Magnetic Resonance Imaging (1994)
    [s.l.] : Nature Publishing Company
    Nature biotechnology 12 (1994), S. 75-78 
    Details
    ISSN: 1546-1696
    Quelle: Nature Archives 1869 - 2009
    Thema: Biologie , Werkstoffwissenschaften, Fertigungsverfahren, Fertigung
    Notizen: [Auszug] We have used diffusion–weighted 1H NMR micro–imaging and localized spectroscopy techniques to monitor the growth and distribution of mammalian cells in a hollow–fiber bioreactor. Non–invasive NMR measurements of this type should also allow investigation of metabolic ...
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1038/nbt0194-75
    Standort Signatur Einschränkungen Verfügbarkeit
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    Artikel (Nationallizenzen)
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  • 2
    Digitale Medien
    Digitale Medien
    Ammonia rhythm in Microcystis firma studied by in vivo 15N and 31P NMR spectroscopy (1991)
    Springer
    Archives of microbiology 156 (1991), S. 471-476 
    Details
    ISSN: 1432-072X
    Schlagwort(e): Ammonia assimilation ; In vivo 15N and 31P NMR spectroscopy ; Amino acid metabolism ; Carbon limitation ; Cyanobacterium ; Microcystis firma
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie
    Notizen: Abstract Cultures of the cyanobacterium Microcystis firma show rhythmic uptake and release of ammonia under conditions of carbon limitation. The massive removal of ammonia from the medium during the first light phase has little impact on the intracellular pH: a pH shift of less than 0.2 U towards the alkaline can be measured by in vivo 31P NMR. Furthermore, the energy status of the cells remains regulated. In vivo 15N NMR of M. firma, cultivated either with labelled nitrate or ammonia as the sole nitrogen source, reveals only gradual differences in the pool of free amino acids. Additionally both cultivation types show γ-aminobutyric acid, acid amides and yet unassigned secondary metabolites as nitrogen storing compounds. Investigating the incorporation of nitrogen under carbon limitation, however, only the amide nitrogen of glutamine is found permanently labelled in situ. While transamination reactions are blocked, nitrate reduction to ammonia can still proceed. Cation exchange processes in the cell wall are considered regarding the ammonia disappearance in the first phase, and the control of ammonia uptake is discussed with respect to the avoidance of intracellular toxification.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF00245394
    Standort Signatur Einschränkungen Verfügbarkeit
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    Artikel (Nationallizenzen)
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  • 3
    Digitale Medien
    Digitale Medien
    Mapping of oxygen tension and cell distribution in a hollow-fiber bioreactor using magnetic resonance imaging (1997)
    New York, NY [u.a.] : Wiley-Blackwell
    Biotechnology and Bioengineering 56 (1997), S. 56-61 
    Details
    ISSN: 0006-3592
    Schlagwort(e): oxygen tension ; cell distribution ; hollow-fiber bioreactor ; magnetic resonance imaging ; Chemistry ; Biochemistry and Biotechnology
    Quelle: Wiley InterScience Backfile Collection 1832-2000
    Thema: Biologie , Werkstoffwissenschaften, Fertigungsverfahren, Fertigung
    Notizen: We mapped the distribution of dissolved oxygen and mammalian cells in a hollow-fiber bioreactor (HFBR) using 19F NMR T1 relaxation time imaging measurements on an infused perfluorocarbon probe molecule and diffusion-weighted 1H NMR imaging of water. This study shows how cell density influences dissolved oxygen concentration in the reactor and demonstrates that NMR can play an important role in defining the biochemical engineering parameters required for optimization of HFBR design and operation. © 1997 John Wiley & Sons, Inc. Biotechnol Bioeng 56: 56-61, 1997.
    Zusätzliches Material: 3 Ill.
    Materialart: Digitale Medien
    Standort Signatur Einschränkungen Verfügbarkeit
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    Artikel (Nationallizenzen)
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