ISSN:
1435-702X
Source:
Springer Online Journal Archives 1860-2000
Topics:
Medicine
Notes:
Abstract • Background: The present study was untertaken in an attempt to broaden the spectrum of known neuronal markers and neuropeptides in the main lacrimal gland of the human by light-microscopic immunohistochemistry. • Methods: Using antisera against the neuronal markers protein gene product (PGP) and S-100 protein (S-100), the distribution of nerve fibers in the human main lacrimal gland was studied. Vasoactive intestinal polypeptide (VIP), calcitonin gene-related peptide (CGRP), neuropeptide Y (NPY) and tyrosine hydroxylase (TH) were identified by their specific antisera. • Results: The nerve fibers are distributed throughout the interstice between the glandular tubules. Associations were also found between nerve fibers and both the interlobular ductal system and blood vessels (mainly arterioles). Within the glandular lobules isolated groups of secretory cells stained positive for S-100 protein. Nerve fibers situated in the glandular interstice between the tubules showed predominantly positive immunoreactions for the neuropeptide VIP, while only very few fibers stained positive for CGRP, NPY and the catecholamine marker TH. Nerve fibers associated with interlobular blood vessels were mainly CGRP and NPY positive and stained only very rarely for VIP. The epithelia of interlobular ducts and excretory ducts were associated with CGRP-immunoreactive nerve fibers. • Conclusion: The neuropeptides identified in the lacrimal gland indicate the complexity with which a variety of biologic signals regulate and modulate the lacrimal gland.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF00430415
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