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  • 14C-leucine incorporation; Bacterial abundance of HDNA-Bacteria; Bacterial abundance of LDNA-Bacteria; bacterial production; BIBS; Bridging in Biodiversity Science; chlorophyll; Climate change; Climate driven Changes in Biodiversity of Microbiota; cyanobacteria; DATE/TIME; Day of experiment; DCM; DEPTH, water; derived; Description; disturbance; enclosure experiment; Enclosure experiment; Epifluorescence microscopy after DAPI staining; Flow cytometry; Free-living bacterial abundance; Germany; lake; Lake_Stechlin; mesocosm experiment; Mesocosm label; NITROLIMIT; Nostocales biovolume; Nostocales cell abundance; Oxygen/Nitrogen ratio; Particle-associated bacterial abundance; Picocyanobacteria abundance; Protein production, free-living bacteria; Protein production, particle associated bacteria; Stickstofflimitation in Binnengewässern; summer storm; TemBi; Treatment  (1)
  • BIBS; Bridging in Biodiversity Science; Calcium carbonate, particulate; Carbon, inorganic, particulate; Carbon, organic, particulate; Carbon, total, particulate; Climate change; Climate changes; Climate driven Changes in Biodiversity of Microbiota; cyanobacteria; DATE/TIME; Day of experiment; deep chlorophyll maximum (DCM); Derived from PIC by molar conversion; Description; Difference derived from TPC and PIC; disturbance; ELTRA-800 (0.3 µm GF-75 filters (Sterlitech)); enclosure experiment; Enclosure experiment; Germany; Infralyt 50 (SAXON Junkalor GmbH), (0.3 µm GF-75 filters (Sterlitech)); Lake_Stechlin; Mesocosm label; mesocosm study; Nitrogen, total, particulate; NITROLIMIT; Oxygen/Nitrogen ratio; Peroxodisulfate oxidation method; Flow-injection analyzer; 0.3 µm GF-75 filters (Sterlitech); Phosphorus, total, particulate; sedimentation; sedimentation rates; Stickstofflimitation in Binnengewässern; TemBi; Treatment  (1)
  • PANGAEA  (2)
Document type
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  • PANGAEA  (2)
Years
  • 1
    Publication Date: 2024-06-12
    Description: We simulated an experimental summer storm in large-volume (~1200 m3, ~16m depth) enclosures in Lake Stechlin (https://www.lake-lab.de) by mixing deeper water masses from the meta- and hypolimnion into the mixed layer (epilimnion). The mixing included the disturbance of a deep chlorophyll maximum (DCM) which was present at the same time of the experiment in Lake Stechlin and situated in the metalimnion of each enclosure during filling. Size-fractionated Bacterial Protein Production (BPP) of particle associated (PA, 〉3.0 µm) and free-living bacteria (FL, 0.2-3.0 µm) (14C-Leu incorporation) as well as abundances of PA (microscopy of DAPI stained cells on 3.0 µm polycarbonate filters) and FL heterotrophic prokaryotes and picocyanobacteria (flow cytometry of SYBR green I stained cells) were monitored for 42 days after the experimental disturbance event. Mixing increased bacterial abundance and production about 3 weeks after mixing, which was associated to a mixing-induced stimulation of phytoplankton growth in the mixed enclosures compared to the controls. Simultaneously, decreased abundances of picocyanobacteria could be observed in mixed enclosures.
    Keywords: 14C-leucine incorporation; Bacterial abundance of HDNA-Bacteria; Bacterial abundance of LDNA-Bacteria; bacterial production; BIBS; Bridging in Biodiversity Science; chlorophyll; Climate change; Climate driven Changes in Biodiversity of Microbiota; cyanobacteria; DATE/TIME; Day of experiment; DCM; DEPTH, water; derived; Description; disturbance; enclosure experiment; Enclosure experiment; Epifluorescence microscopy after DAPI staining; Flow cytometry; Free-living bacterial abundance; Germany; lake; Lake_Stechlin; mesocosm experiment; Mesocosm label; NITROLIMIT; Nostocales biovolume; Nostocales cell abundance; Oxygen/Nitrogen ratio; Particle-associated bacterial abundance; Picocyanobacteria abundance; Protein production, free-living bacteria; Protein production, particle associated bacteria; Stickstofflimitation in Binnengewässern; summer storm; TemBi; Treatment
    Type: Dataset
    Format: text/tab-separated-values, 2003 data points
    Location Call Number Limitation Availability
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  • 2
    Publication Date: 2024-06-12
    Description: We simulated an experimental summer storm in large-volume (~1200 m3, ~16m depth) enclosures in Lake Stechlin (https://www.lake-lab.de) by mixing deeper water masses from the meta- and hypolimnion into the mixed layer (epilimnion). The mixing included the disturbance of a deep chlorophyll maximum (DCM) which was present at the same time of the experiment in Lake Stechlin and situated in the metalimnion of each enclosure during filling. Water physical variables and water chemistry was monitored for 42 days after the experimental disturbance event. Mixing disrupted the thermal stratification, increased concentrations of dissolved nutrients and CO2 and changed light conditions in the epilimnion. Mixing stimulated phytoplankton growth, thus, resulting in a bloom of Dolichospermum sp. and thereafter increased biomass of Bacillariophyceae. Subsequent, break down of both phytoplankton groups resulted in higher particulate matter sinking fluxes of particulate organic carbon (POC), total particulate nitrogen (TPN) and total particulate phosphorous (TPP) 4-5 weeks after the disturbance event. Mixing resulted in average increases in elemental downward fluxes of 9% POC, 14% total particulate Nitrogen (TPN) and 19% TPP by the end of the experiment (42 days) (n.control=4, n.mixed=4).
    Keywords: BIBS; Bridging in Biodiversity Science; Calcium carbonate, particulate; Carbon, inorganic, particulate; Carbon, organic, particulate; Carbon, total, particulate; Climate change; Climate changes; Climate driven Changes in Biodiversity of Microbiota; cyanobacteria; DATE/TIME; Day of experiment; deep chlorophyll maximum (DCM); Derived from PIC by molar conversion; Description; Difference derived from TPC and PIC; disturbance; ELTRA-800 (0.3 µm GF-75 filters (Sterlitech)); enclosure experiment; Enclosure experiment; Germany; Infralyt 50 (SAXON Junkalor GmbH), (0.3 µm GF-75 filters (Sterlitech)); Lake_Stechlin; Mesocosm label; mesocosm study; Nitrogen, total, particulate; NITROLIMIT; Oxygen/Nitrogen ratio; Peroxodisulfate oxidation method; Flow-injection analyzer; 0.3 µm GF-75 filters (Sterlitech); Phosphorus, total, particulate; sedimentation; sedimentation rates; Stickstofflimitation in Binnengewässern; TemBi; Treatment
    Type: Dataset
    Format: text/tab-separated-values, 527 data points
    Location Call Number Limitation Availability
    BibTip Others were also interested in ...
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