Description: Author Posting. © The Oceanography Society, 2014. This article is posted here by permission of The Oceanography Society for personal use, not for redistribution. The definitive version was published in Oceanography 27, no. 2 (2014): 18-23, doi:10.5670/oceanog.2014.56.

Description: Continental shelves and the waters overlying them support numerous industries as diverse as tourism and recreation, energy extraction, fisheries, transportation, and applications of marine bio-molecules (e.g., agribusiness, food processing, pharmaceuticals). Although these shelf ecosystems exhibit impacts of climate change and increased human use of resources (Halpern et al., 2012; IPCC, 2013, 2014; Melillo et al., 2014), there are currently no standardized metrics for assessing changes in ecological function in the coastal ocean. Here, we argue that it is possible to monitor vital signs of ecosystem function by focusing on the lowest levels of the ocean food web. Establishment of biodiversity, biomass, and primary productivity baselines and continuous evaluation of changes in biological resources in these economically and ecologically valuable regions requires an internationally coordinated monitoring effort that fully integrates natural, social, and economic sciences to jointly identify problems and design solutions. Such an ocean observing network is needed to protect the livelihoods of coastal communities in the context of the goals of the Future Earth program (Mooney et al., 2013) and of the Intergovernmental Platform on Biodiversity and Ecosystem Services (http://www.ipbes.net). The tools needed to initiate these assessments are available today.

Description: AEW and RML have been supported by C-MORE (NSF) and the Gordon and Betty Moore and Alfred P. Sloan Foundations. FMK and EM have been supported by NASA, NOAA, NSF, and EPA. FPC was supported by the David and Lucile Packard Foundation and NASA. HMS was supported by NASA and the Gordon and Betty Moore Foundation. EMJ received support from NOAA. MB received support from the NSF. MTK and SCD acknowledge support from C-MORE (NSF). MWL was supported by NSF and NASA. WMB was supported by NASA and NSF.

Description: Eastern boundary upwelling systems (EBUS) contribute a disproportionate fraction of the global fish catch relative to their size and are especially susceptible to global environmental change. Here we present the evolution of communities over 50 days in an in situ mesocosm 6 km offshore of Callao, Peru and in the nearby unenclosed coastal Pacific Ocean. The communities were monitored using multi-marker environmental DNA (eDNA) metabarcoding and flow cytometry. DNA extracted from weekly water samples were subjected to amplicon sequencing for four genetic loci: 1) the V1-V2 region of the 16S rRNA gene, for photosynthetic eukaryotes (via their chloroplasts) and bacteria; 2) the V9 region of the 18S rRNA gene for exploration of eukaryotes but targeting phytoplankton; 3) cytochrome oxidase I (COI), for exploration of eukaryotic taxa but targeting invertebrates, and 4) the 12S rRNA gene, targeting vertebrates. The multi-marker approach showed a divergence of communities (from microbes to fish) between the mesocosm and the unenclosed ocean. Together with the environmental information, the genetic data furthered our mechanistic understanding of the processes that are shaping EBUS communities in a changing ocean. The unenclosed ocean experienced significant variability over the course of the 50-day experiment with temporal shifts in community composition but remained dominated by organisms that are characteristic of high nutrient, upwelling conditions (e.g. diatoms, copepods, anchovies). A large directional change was found in the mesocosm community. The mesocosm community that developed was characteristic of upwelling regions when upwelling relaxes and waters stratify (e.g. dinoflagellates, nanoflagellates). The selection of dinoflagellates under the warm (coastal El Niño) and stratified conditions in the mesocosm may be an indication of how EBUS will respond under the global environmental changes (i.e. continued global warming) forecast by the IPCC.